{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:31:09Z","timestamp":1760236269874,"version":"build-2065373602"},"reference-count":26,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T00:00:00Z","timestamp":1636329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Interstitial cystitis (IC) is a chronic inflammatory disease characterized by bladder pain and increased urinary frequency. Although the C57BL\/6J (B6) and FVB\/NJ (FVB) mouse strains are commonly used as animal models for studies involving the urinary system, few reports have compared their lower urinary tract anatomy, despite the importance of such data. Our study aimed to characterize bladder function changes in FVB and B6 mouse strains with lipopolysaccharide (LPS)-induced IC, to understand mouse model-based bladder research. The bladder function parameters were measured by cystometrogram. Histological assay was examined by hematoxylin and eosin stain, Masson\u2019s trichrome stain, and immunofluorescence staining. Results indicated that the two strains in the control group exhibited different bladder structures and functions, with significant anatomical differences, including a larger bladder size in the FVB than in the B6 strain. Furthermore, cystometry tests revealed differences in bladder function pressure. LPS-treated B6 mice presented significant changes in peak pressure, with decreased intercontraction intervals; these results were similar to symptoms of IC in humans. Each strain displayed distinct characteristics, emphasizing the care required in choosing the appropriate strain for bladder-model studies. The results suggested that the B6 mouse strain is more suitable for IC models.<\/jats:p>","DOI":"10.3390\/ijms222112053","type":"journal-article","created":{"date-parts":[[2021,11,7]],"date-time":"2021-11-07T20:42:54Z","timestamp":1636317774000},"page":"12053","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model"],"prefix":"10.3390","volume":"22","author":[{"given":"Ching-Hao","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, Sheffield University, Sheffield S10 2TN, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Hou","family":"Liao","sequence":"additional","affiliation":[{"name":"Division of Urology, Department of Surgery, Cardinal Tien Hospital, New Taipei City 231, Taiwan"},{"name":"School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo-Chiang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan"},{"name":"Department of Urology, Cathay General Hospital, Taipei City 106, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuan-Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Wen","family":"Tseng","sequence":"additional","affiliation":[{"name":"Program in Pharmaceutical Biotechnology, College of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Kung","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Urology, Mackay Memorial Hospital, Taipei City 104, Taiwan"},{"name":"Ph.D. Program in Nutrition and Food Science, Graduate Institute of Biomedical and Pharmaceutical Science, Fu Jen Catholic University, New Taipei City 242, Taiwan"},{"name":"Department of Medicine, Mackay Medical College, New Taipei City 251, Taiwan"},{"name":"Mackay Junior College of Medicine, Nursing, and Management, Taipei City 104, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han-Sun","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Urology, Cathay General Hospital, Taipei City 106, Taiwan"},{"name":"Graduate Institute of Biomedical and Pharmaceutical Science, Fu Jen Catholic University, New Taipei City 242, Taiwan"},{"name":"Department of Urology, Fu Jen Catholic University Hospital, New Taipei City 242, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-No","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1016\/S0022-5347(17)35629-X","article-title":"A quantitatively controlled method to study prospectively interstitial cystitis and demonstrate the efficacy of pentosanpolysulfate","volume":"150","author":"Parsons","year":"1993","journal-title":"J. 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