{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:48:49Z","timestamp":1790185729508,"version":"4.1.0"},"reference-count":103,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T00:00:00Z","timestamp":1637625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/S03305X\/1"],"award-info":[{"award-number":["EP\/S03305X\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Current approaches for bladder reconstruction surgery are associated with many morbidities. Tissue engineering is considered an ideal approach to create constructs capable of restoring the function of the bladder wall. However, many constructs to date have failed to create a sufficient improvement in bladder capacity due to insufficient neobladder compliance. This review evaluates the biomechanical properties of the bladder wall and how the current reconstructive materials aim to meet this need. To date, limited data from mechanical testing and tissue anisotropy make it challenging to reach a consensus on the native properties of the bladder wall. Many of the materials whose mechanical properties have been quantified do not fall within the range of mechanical properties measured for native bladder wall tissue. Many promising new materials have yet to be mechanically quantified, which makes it difficult to ascertain their likely effectiveness. The impact of scaffold structures and the long-term effect of implanting these materials on their inherent mechanical properties are areas yet to be widely investigated that could provide important insight into the likely longevity of the neobladder construct. In conclusion, there are many opportunities for further investigation into novel materials for bladder reconstruction. Currently, the field would benefit from a consensus on the target values of key mechanical parameters for bladder wall scaffolds.<\/jats:p>","DOI":"10.3390\/ijms222312657","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T04:48:37Z","timestamp":1638247717000},"page":"12657","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["The Significance of Biomechanics and Scaffold Structure for Bladder Tissue Engineering"],"prefix":"10.3390","volume":"22","author":[{"given":"Marta","family":"Hanczar","sequence":"first","affiliation":[{"name":"Applied Biomedical Engineering Group, Centre for Precision Healthcare, UCL Division of Medicine, University College London, London WC1E 6JF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9951-2975","authenticated-orcid":false,"given":"Mehran","family":"Moazen","sequence":"additional","affiliation":[{"name":"UCL Department of Mechanical Engineering, University College London, London WC1E 7JE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3124-2294","authenticated-orcid":false,"given":"Richard","family":"Day","sequence":"additional","affiliation":[{"name":"Applied Biomedical Engineering Group, Centre for Precision Healthcare, UCL Division of Medicine, University College London, London WC1E 6JF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.1016\/j.biomaterials.2004.07.011","article-title":"Three-dimensional, nano-structured PLGA scaffolds for bladder tissue replacement applications","volume":"26","author":"Pattison","year":"2005","journal-title":"Biomaterials"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1111\/j.1464-410X.2011.10650.x","article-title":"The past, present and future of augmentation cystoplasty","volume":"109","author":"Biers","year":"2012","journal-title":"BJU Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.1002\/sctm.17-0101","article-title":"Concise review: Tissue engineering of urinary bladder; we still have a long way to go?","volume":"6","author":"Adamowicz","year":"2017","journal-title":"Stem Cells Transl. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.eururo.2013.09.042","article-title":"Analysis of intracorporeal compared with extracorporeal urinary diversion after robot-assisted radical cystectomy: Results from the International Robotic Cystectomy Consortium","volume":"65","author":"Ahmed","year":"2014","journal-title":"Eur. Urol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.suc.2016.02.011","article-title":"The use of bowel in urologic reconstructive surgery","volume":"96","author":"Hansen","year":"2016","journal-title":"Surg. Clin. N. Am."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1111\/bju.12239","article-title":"Risk of in-hospital complications after radical cystectomy for urinary bladder carcinoma: Population-based follow-up study of 7608 patients","volume":"112","author":"Thorstenson","year":"2013","journal-title":"BJU Int."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"316","DOI":"10.4111\/icu.2016.57.5.316","article-title":"Augmentation cystoplasty in neurogenic bladder","volume":"57","author":"Kocjancic","year":"2016","journal-title":"Investig. Clin. Urol."},{"key":"ref_8","first-page":"67","article-title":"Complications of radical cystectomy","volume":"59","author":"Buscarini","year":"2007","journal-title":"Minerva Urol. Nefrol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1002\/term.2812","article-title":"Reconstructive urology and tissue engineering: Converging developmental paths","volume":"13","author":"Adamowicz","year":"2019","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/S0022-5347(17)66624-2","article-title":"Experience with urinary bladder regeneration","volume":"77","author":"Bohne","year":"1957","journal-title":"J. Urol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1111\/j.1464-410X.1958.tb06231.x","article-title":"Vesical regeneration in the human after total cystectomy and implantation of a plastic mould","volume":"30","author":"Sanchez","year":"1958","journal-title":"Br. J. Urol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1111\/j.1464-410X.1964.tb09488.x","article-title":"Formation of a bladder by a plastic shell after total cystectomy","volume":"36","author":"Tsulukidze","year":"1964","journal-title":"Br. J. Urol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0022-5347(17)61680-X","article-title":"Enlargement of contracted bladder by use of gelatin sponge bladder","volume":"104","author":"Orikasa","year":"1970","journal-title":"J. Urol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0022-5347(17)62828-3","article-title":"Clinical experiences of bladder reconstruction using preserved bladder and gelatin sponge bladder in the case of bladder cancer","volume":"98","author":"Tsuji","year":"1967","journal-title":"J. Urol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/S0022-5347(17)58181-1","article-title":"Cystoplasty by regeneration of the bladder","volume":"118","author":"Taguchi","year":"1977","journal-title":"J. Urol."},{"key":"ref_16","first-page":"355","article-title":"The use of resin-sprayed thin paper for urinary bladder regeneration","volume":"15","author":"Fujita","year":"1978","journal-title":"Investig. Urol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1159\/000455617","article-title":"Duraplasty of the urinary bladder\u2014results after two to six years","volume":"1","year":"1975","journal-title":"Eur. Urol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/j.1442-2042.1995.tb00448.x","article-title":"Augmentation duracystoplasty in neurogenic bladder dysfunction","volume":"2","author":"Arikan","year":"1995","journal-title":"Int. J. Urol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"150","DOI":"10.4111\/kju.2011.52.2.150","article-title":"Bladder reconstruction using bovine pericardium in a case of enterovesical fistula","volume":"52","author":"Moon","year":"2011","journal-title":"Korean J. Urol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s00383-012-3063-0","article-title":"Bladder augmentation using acellular collagen biomatrix: A pilot experience in exstrophic patients","volume":"28","author":"Caione","year":"2012","journal-title":"Pediatr. Surg. Int."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/nrurol.2018.5","article-title":"Bladder biomechanics and the use of scaffolds for regenerative medicine in the urinary bladder","volume":"15","author":"Ajalloueian","year":"2018","journal-title":"Nat. Rev. Urol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.addr.2011.03.004","article-title":"Vascularization is the key challenge in tissue engineering","volume":"63","author":"Novosel","year":"2011","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1007\/s00345-019-02833-4","article-title":"Review of clinical experience on biomaterials and tissue engineering of urinary bladder","volume":"38","author":"Chua","year":"2020","journal-title":"World J. Urol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"111867","DOI":"10.1016\/j.msec.2021.111867","article-title":"The determinant role of fabrication technique in final characteristics of scaffolds for tissue engineering applications: A focus on silk fibroin-based scaffolds","volume":"122","author":"Khademolqorani","year":"2021","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"F1477","DOI":"10.1152\/ajprenal.00327.2009","article-title":"Cell biology and physiology of the uroepithelium","volume":"297","author":"Khandelwal","year":"2009","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1007.e1","DOI":"10.1016\/j.urology.2010.11.036","article-title":"Evaluation of viability and proliferative activity of human urothelial cells cultured onto xenogenic tissue-engineered extracellular matrices","volume":"77","author":"Davis","year":"2011","journal-title":"Urology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.eururo.2015.07.071","article-title":"Summary of European Association of Urology (EAU) Guidelines on Neuro-Urology","volume":"69","author":"Groen","year":"2016","journal-title":"Eur. Urol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zhang, Y., and Atala, A. (2019). Regenerative medicine of the bladder. Principles of Regenerative Medicine, Elsevier.","DOI":"10.1016\/B978-0-12-809880-6.00072-2"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s13293-018-0204-8","article-title":"Sex differences in lower urinary tract biology and physiology","volume":"9","author":"Abelson","year":"2018","journal-title":"Biol. Sex Differ."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s10237-017-0968-5","article-title":"Layer-dependent role of collagen recruitment during loading of the rat bladder wall","volume":"17","author":"Cheng","year":"2018","journal-title":"Biomech. Model. Mechanobiol."},{"key":"ref_31","unstructured":"Bolla, S.R., Odeluga, N., and Jetti, R. (2020). Histology, Bladder, StatPearls Publishing."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1016\/j.transproceed.2012.01.128","article-title":"Urine is a highly cytotoxic agent: Does it influence stem cell therapies in urology?","volume":"44","author":"Adamowicz","year":"2012","journal-title":"Transplant. Proc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1083\/jcb.151.5.961","article-title":"Ablation of uroplakin III gene results in small urothelial plaques, urothelial leakage, and vesicoureteral reflux","volume":"151","author":"Hu","year":"2000","journal-title":"J. Cell Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"F1200","DOI":"10.1152\/ajprenal.00043.2002","article-title":"Role of membrane proteins in permeability barrier function: Uroplakin ablation elevates urothelial permeability","volume":"283","author":"Hu","year":"2002","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"ref_35","unstructured":"Abrams, P. (2006). Urodynamics, Springer."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1002\/nau.22465","article-title":"Lamina propria: The functional center of the bladder?","volume":"33","author":"Andersson","year":"2014","journal-title":"Neurourol. Urodyn."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1002\/(SICI)1097-0185(19990301)254:3<375::AID-AR8>3.0.CO;2-R","article-title":"Microvascular architecture of the human urinary bladder wall: A corrosion casting study","volume":"254","author":"Litwin","year":"1999","journal-title":"Anat. Rec."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Boerckel, J.D., Gemmiti, C.V., Mason, D.E., Kolambkar, Y.M., Porter, B.D., and Guldberg, R.E. (2019). Physical stress as a factor in tissue growth and remodeling. Principles of Regenerative Medicine, Elsevier.","DOI":"10.1016\/B978-0-12-809880-6.00026-6"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1152\/ajplegacy.1971.220.5.1413","article-title":"Mechanical properties of urinary bladder","volume":"220","author":"Alexander","year":"1971","journal-title":"Am. J. Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1115\/1.3138285","article-title":"Biomechanics: Mechanical properties of living tissues","volume":"103","author":"Fung","year":"1981","journal-title":"J. Biomech. Eng."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1114\/B:ABME.0000042228.89106.48","article-title":"Changes in the biaxial viscoelastic response of the urinary bladder following spinal cord injury","volume":"32","author":"Nagatomi","year":"2004","journal-title":"Ann. Biomed. Eng."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s10237-007-0095-9","article-title":"Contribution of the extracellular matrix to the viscoelastic behavior of the urinary bladder wall","volume":"7","author":"Nagatomi","year":"2008","journal-title":"Biomech. Model Mechanobiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Hamlin, M. (2013). The physiology of sports injuries and repair processes. Current Issues in Sports and Exercise Medicine, BoD\u2014Books on Demand.","DOI":"10.5772\/56649"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"49","DOI":"10.3233\/BIR-2012-0604","article-title":"Bladder tissue passive response to monotonic and cyclic loading","volume":"49","author":"Zanetti","year":"2012","journal-title":"Biorheology"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Winkelstein, B. (2012). Orthopaedic Biomechanics, CRC Press.","DOI":"10.1201\/b13733"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1016\/S0142-9612(02)00536-7","article-title":"Comparison of the mechanical behaviors of biological tissues subjected to uniaxial tensile testing: Pig, calf and ostrich pericardium sutured with Gore-Tex","volume":"24","author":"Cordon","year":"2003","journal-title":"Biomaterials"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1046\/j.1464-410X.1998.00748.x","article-title":"Composition and biomechanical properties of the bladder acellular matrix graft: Comparative analysis in rat, pig and human","volume":"82","author":"Dahms","year":"1998","journal-title":"Br. J. Urol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.biomaterials.2008.09.034","article-title":"Regional biomechanical and histological characterisation of the passive porcine urinary bladder: Implications for augmentation and tissue engineering strategies","volume":"30","author":"Korossis","year":"2009","journal-title":"Biomaterials"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1007\/s00192-011-1409-0","article-title":"Uniaxial mechanical behavior of the human female bladder","volume":"22","author":"Martins","year":"2011","journal-title":"Int. Urogynecol. J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"8681","DOI":"10.1016\/j.biomaterials.2013.08.001","article-title":"Bladder tissue regeneration using acellular bi-layer silk scaffolds in a large animal model of augmentation cystoplasty","volume":"34","author":"Tu","year":"2013","journal-title":"Biomaterials"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jmbbm.2017.11.034","article-title":"Bladder wall biomechanics: A comprehensive study on fresh porcine urinary bladder","volume":"79","author":"Jokandan","year":"2018","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1046\/j.1464-4096.2001.001206","article-title":"Augmentation cystoplasty","volume":"88","author":"Greenwell","year":"2001","journal-title":"BJU Int."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Kristensen, J.H., and Karsdal, M.A. (2016). Elastin. Biochemistry of Collagens, Laminins and Elastin, Elsevier.","DOI":"10.1016\/B978-0-12-809847-9.00030-1"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2752","DOI":"10.1016\/j.jbiomech.2013.07.022","article-title":"Murine bladder wall biomechanics following partial bladder obstruction","volume":"46","author":"Chen","year":"2013","journal-title":"J. Biomech."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1016\/j.jbiomech.2010.02.034","article-title":"Ex vivo deformations of the urinary bladder wall during whole bladder filling: Contributions of extracellular matrix and smooth muscle","volume":"43","author":"Parekh","year":"2010","journal-title":"J. Biomech."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3088","DOI":"10.1016\/j.jbiomech.2015.07.021","article-title":"Bladder tissue biomechanical behavior: Experimental tests and constitutive formulation","volume":"48","author":"Natali","year":"2015","journal-title":"J. Biomech."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1002\/nau.20584","article-title":"The significance of natural bladder filling by the production of urine during cystometry","volume":"27","author":"Lee","year":"2008","journal-title":"Neurourol. Urodyn."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1016\/S0022-5347(05)65137-3","article-title":"Passive biaxial mechanical properties of the rat bladder wall after spinal cord injury","volume":"167","author":"Gloeckner","year":"2002","journal-title":"J. Urol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5518","DOI":"10.1016\/j.biomaterials.2005.01.070","article-title":"Analytically derived material properties of multilaminated extracellular matrix devices using the ball-burst test","volume":"26","author":"Freytes","year":"2005","journal-title":"Biomaterials"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1002\/jbm.b.30885","article-title":"Uniaxial and biaxial properties of terminally sterilized porcine urinary bladder matrix scaffolds","volume":"84","author":"Freytes","year":"2008","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2353","DOI":"10.1016\/j.biomaterials.2003.09.015","article-title":"Biaxial strength of multilaminated extracellular matrix scaffolds","volume":"25","author":"Freytes","year":"2004","journal-title":"Biomaterials"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1002\/(SICI)1097-4636(199823)43:3<277::AID-JBM8>3.0.CO;2-K","article-title":"Multilaminate resorbable biomedical device under biaxial loading","volume":"43","author":"Whitson","year":"1998","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Brookoff, D. (2009). Genitourinary pain syndromes: Interstitial cystitis, chronic prostatitis, pelvic floor dysfunction, and related disorders. Current Therapy in Pain, Elsevier.","DOI":"10.1016\/B978-1-4160-4836-7.00028-6"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Pfeiffer, R.F. (2007). Bladder and sexual function and dysfunction. Neurology and Clinical Neuroscience, Elsevier.","DOI":"10.1016\/B978-0-323-03354-1.50033-X"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/(SICI)1520-6777(1996)15:5<483::AID-NAU5>3.0.CO;2-B","article-title":"Bladder compliance in neurologically intact women","volume":"15","author":"Harris","year":"1996","journal-title":"Neurourol. Urodyn."},{"key":"ref_66","unstructured":"Boron, W.F., and Boulpaep, E.L. (2017). Medical Physiology, Elsevier."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Zhu, Y., and Wagner, W.R. (2019). Design principles in biomaterials and scaffolds. Principles of Regenerative Medicine, Elsevier.","DOI":"10.1016\/B978-0-12-809880-6.00030-8"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1016\/j.juro.2013.10.103","article-title":"Autologous cell seeded biodegradable scaffold for augmentation cystoplasty: Phase II study in children and adolescents with spina bifida","volume":"191","author":"Joseph","year":"2014","journal-title":"J. Urol."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Scarritt, M., Murdock, M., and Badylak, S.F. (2019). Biologic scaffolds composed of extracellular matrix for regenerative medicine. Principles of Regenerative Medicine, Elsevier.","DOI":"10.1016\/B978-0-12-809880-6.00035-7"},{"key":"ref_70","first-page":"4043","article-title":"Characterization of different biodegradable scaffolds in tissue engineering","volume":"19","author":"Qiu","year":"2019","journal-title":"Mol. Med. Rep."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.biomaterials.2010.09.006","article-title":"The effect of source animal age upon extracellular matrix scaffold properties","volume":"32","author":"Tottey","year":"2011","journal-title":"Biomaterials"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1163\/156856208784089599","article-title":"Hybrid nanofibrous scaffolds from electrospinning of a synthetic biodegradable elastomer and urinary bladder matrix","volume":"19","author":"Stankus","year":"2008","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1089\/ten.tec.2012.0633","article-title":"One-stage tissue engineering of bladder wall patches for an easy-to-use approach at the surgical table","volume":"19","author":"Ajalloueian","year":"2013","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"5741","DOI":"10.1016\/j.biomaterials.2014.04.002","article-title":"Constructs of electrospun PLGA, compressed collagen and minced urothelium for minimally manipulated autologous bladder tissue expansion","volume":"35","author":"Ajalloueian","year":"2014","journal-title":"Biomaterials"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.actbio.2015.05.032","article-title":"Time-dependent bladder tissue regeneration using bilayer bladder acellular matrix graft-silk fibroin scaffolds in a rat bladder augmentation model","volume":"23","author":"Zhao","year":"2015","journal-title":"Acta Biomater."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1080\/09205063.2014.989482","article-title":"Tetronic\u00ae-based composite hydrogel scaffolds seeded with rat bladder smooth muscle cells for urinary bladder tissue engineering applications","volume":"26","author":"Sivaraman","year":"2015","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_77","first-page":"3285","article-title":"Nanostructured polyurethane-poly-lactic-co-glycolic acid scaffolds increase bladder tissue regeneration: An in vivo study","volume":"8","author":"Yao","year":"2013","journal-title":"Int. J. Nanomed."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"045013","DOI":"10.1088\/1748-6041\/8\/4\/045013","article-title":"Evaluation of electrospun bioresorbable scaffolds for tissue-engineered urinary bladder augmentation","volume":"8","author":"Vianello","year":"2013","journal-title":"Biomed. Mater."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1007\/s10439-018-02182-0","article-title":"Evaluation of Poly (Carbonate-Urethane) Urea (PCUU) Scaffolds for Urinary Bladder Tissue Engineering","volume":"47","author":"Sivaraman","year":"2019","journal-title":"Ann. Biomed. Eng."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1016\/j.biomaterials.2012.10.075","article-title":"A bilayered hybrid microfibrous PLGA\u2014Acellular matrix scaffold for hollow organ tissue engineering","volume":"34","author":"Horst","year":"2013","journal-title":"Biomaterials"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.biomaterials.2004.05.006","article-title":"Biaxial mechanical properties of muscle-derived cell seeded small intestinal submucosa for bladder wall reconstitution","volume":"26","author":"Lu","year":"2005","journal-title":"Biomaterials"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/j.jpurol.2012.12.001","article-title":"Bladder augmentation with small intestinal submucosa leads to unsatisfactory long-term results","volume":"9","author":"Schaefer","year":"2013","journal-title":"J. Pediatr. Urol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"4758","DOI":"10.1016\/j.biomaterials.2013.03.038","article-title":"The performance of silk scaffolds in a rat model of augmentation cystoplasty","volume":"34","author":"Seth","year":"2013","journal-title":"Biomaterials"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"9311","DOI":"10.1016\/j.biomaterials.2014.07.057","article-title":"The promotion of functional urinary bladder regeneration using anti-inflammatory nanofibers","volume":"35","author":"Bury","year":"2014","journal-title":"Biomaterials"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Pokrywczynska, M., Jundzill, A., Adamowicz, J., Kowalczyk, T., Warda, K., Rasmus, M., Buchholz, L., Krzyzanowska, S., Nakielski, P., and Chmielewski, T. (2014). Is the poly (L-lactide-co-caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0105295"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/j.biomaterials.2010.09.051","article-title":"Evaluation of gel spun silk-based biomaterials in a murine model of bladder augmentation","volume":"32","author":"Mauney","year":"2011","journal-title":"Biomaterials"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1016\/S0022-5347(01)66117-2","article-title":"Regenerative urinary bladder augmentation using small intestinal submucosa: Urodynamic and histopathologic assessment in long-term canine bladder augmentations","volume":"155","author":"Kropp","year":"1996","journal-title":"J. Urol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1111\/j.1464-410X.2005.05741.x","article-title":"Growth of bone marrow stromal cells on small intestinal submucosa: An alternative cell source for tissue engineered bladder","volume":"96","author":"Zhang","year":"2005","journal-title":"BJU Int."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1111\/j.1464-410X.2006.06447.x","article-title":"Challenges in a larger bladder replacement with cell-seeded and unseeded small intestinal submucosa grafts in a subtotal cystectomy model","volume":"98","author":"Zhang","year":"2006","journal-title":"BJU Int."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1007\/s00383-006-1705-9","article-title":"In vivo bladder regeneration using small intestinal submucosa: Experimental study","volume":"22","author":"Caione","year":"2006","journal-title":"Pediatr. Surg. Int."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1111\/j.1464-410X.2009.08965.x","article-title":"Regional variations in small intestinal submucosa evoke differences in inflammation with subsequent impact on tissue regeneration in the rat bladder augmentation model","volume":"105","author":"Ashley","year":"2010","journal-title":"BJU Int."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1111\/j.1464-410X.2010.09757.x","article-title":"Bladder regeneration in a canine model using hyaluronic acid-poly(lactic-co-glycolic-acid) nanoparticle modified porcine small intestinal submucosa","volume":"108","author":"Roth","year":"2011","journal-title":"BJU Int."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1002\/stem.568","article-title":"A nonhuman primate model for urinary bladder regeneration using autologous sources of bone marrow-derived mesenchymal stem cells","volume":"29","author":"Sharma","year":"2011","journal-title":"Stem Cells"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1038\/sj.ijir.3901587","article-title":"Tunica repair with acellular bladder matrix maintains corporal tissue function","volume":"19","author":"Eberli","year":"2007","journal-title":"Int. J. Impot. Res."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1158\/1940-6207.CAPR-19-0093","article-title":"Scented Candles as an Unrecognized Factor that Increases the Risk of Bladder Cancer; Is There Enough Evidence to Raise a Red Flag?","volume":"12","author":"Adamowicz","year":"2019","journal-title":"Cancer Prev. Res."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0090-4295(97)00644-4","article-title":"Bladder augmentation using allogenic bladder submucosa seeded with cells","volume":"51","author":"Yoo","year":"1998","journal-title":"Urology"},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Coutu, D.L., Mahfouz, W., Loutochin, O., Galipeau, J., and Corcos, J. (2014). Tissue engineering of rat bladder using marrow-derived mesenchymal stem cells and bladder acellular matrix. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0111966"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"613439","DOI":"10.1155\/2015\/613439","article-title":"Application of bladder acellular matrix in urinary bladder regeneration: The state of the art and future directions","volume":"2015","author":"Pokrywczynska","year":"2015","journal-title":"BioMed Res. Int."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.juro.2014.03.116","article-title":"Tissue engineering of the bladder\u2014Reality or myth? A systematic review","volume":"192","author":"Sloff","year":"2014","journal-title":"J. Urol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"160ps23","DOI":"10.1126\/scitranslmed.3003688","article-title":"Building vascular networks","volume":"4","author":"Bae","year":"2012","journal-title":"Sci. Transl. Med."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"5824","DOI":"10.1038\/s41598-020-62197-3","article-title":"Development of a conductive biocomposite combining graphene and amniotic membrane for replacement of the neuronal network of tissue-engineered urinary bladder","volume":"10","author":"Adamowicz","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1080\/14712598.2018.1439013","article-title":"Bladder regeneration through stem cell therapy","volume":"18","author":"Smolar","year":"2018","journal-title":"Expert Opin. Biol. Ther."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"458","DOI":"10.2174\/1574888X13666180426113907","article-title":"The role of stem cells for reconstructing the lower urinary tracts","volume":"13","author":"Davis","year":"2018","journal-title":"Curr. Stem Cell Res. Ther."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/23\/12657\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:34:51Z","timestamp":1760168091000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/23\/12657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,23]]},"references-count":103,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["ijms222312657"],"URL":"https:\/\/doi.org\/10.3390\/ijms222312657","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,23]]}}}