{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:07:19Z","timestamp":1789664839420,"version":"4.0.1"},"reference-count":66,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T00:00:00Z","timestamp":1637798400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Inflammatory bowel diseases (IBD) are chronic, immune-mediated disorders, which affect the gastrointestinal tract with intermittent ulceration. It is increasingly clear that neutrophil extracellular traps (NETs) seem to have a role in IBD; however, the associated pathogenesis is still not known. Furthermore, several conventional therapies are available against IBD, although these might have side effects. Our current study aimed to investigate the effects of hydrogen sulfide (H2S) treatment on NETs formation and on the expression of inflammatory mediators in experimental rat colitis. To model IBD, 2,4,6-trinitrobenzenesulfonic acid (TNBS) was administered intracolonically (i.c.) to Wistar\u2013Harlan male rats. Animals were treated (2 times\/day) with H2S donor Lawesson\u2019s reagent per os. Our results showed that H2S treatment significantly decreased the extent of colonic lesions. Furthermore, the expression of members of NETs formation: peptidyl arginine deiminase 4 (PAD4), citrullinated histone H3 (citH3), myeloperoxidase (MPO) and inflammatory regulators, such as nuclear transcription factor-kappa B (NF-\u03baB) and high-mobility group box 1 (HMGB1) were reduced in H2S treated group compared to TNBS. Additionally, H2S donor administration elevated the expression of ubiquitin C-terminal hydroxylase L1 (UCHL-1), a potential anti-inflammatory mediator. Taken together, our results showed that H2S may exert anti-inflammatory effect through the inhibition of NETs formation, which suggests a new therapeutic approach against IBD.<\/jats:p>","DOI":"10.3390\/ijms222312729","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T04:48:37Z","timestamp":1638247717000},"page":"12729","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis"],"prefix":"10.3390","volume":"22","author":[{"given":"Szilvia","family":"T\u00f6r\u00f6k","sequence":"first","affiliation":[{"name":"Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikoletta","family":"Alm\u00e1si","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsuzsanna","family":"Valkusz","sequence":"additional","affiliation":[{"name":"Albert Szent-Gy\u00f6rgyi Clinical Center, Department of Medicine, Medical Faculty, University of Szeged, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anik\u00f3","family":"P\u00f3sa","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary"},{"name":"Interdisciplinary Excellence Center, Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Csaba","family":"Varga","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krisztina","family":"Kupai","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary"},{"name":"Albert Szent-Gy\u00f6rgyi Clinical Center, Department of Medicine, Medical Faculty, University of Szeged, H-6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1016\/S0140-6736(17)32448-0","article-title":"Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: A systematic review of population-based studies","volume":"390","author":"Ng","year":"2017","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1002\/ibd.21048","article-title":"World Gastroenterology Organization Practice Guidelines for the diagnosis and management of IBD in 2010","volume":"16","author":"Bernstein","year":"2010","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1056\/NEJMra020831","article-title":"Inflammatory bowel disease","volume":"347","author":"Podolsky","year":"2002","journal-title":"N. Engl. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.pop.2011.06.001","article-title":"Inflammatory bowel disease","volume":"38","author":"Walsh","year":"2011","journal-title":"Prim. Care"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S1734-1140(11)70575-8","article-title":"Treatment of inflammatory bowel disease (IBD)","volume":"63","author":"Pithadia","year":"2011","journal-title":"Pharmacol. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1128\/CMR.15.1.79-94.2002","article-title":"Clinical aspects and pathophysiology of inflammatory bowel disease","volume":"15","author":"Hendrickson","year":"2002","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"279","DOI":"10.4196\/kjpp.2014.18.4.279","article-title":"A review on chemical-induced inflammatory bowel disease models in rodents","volume":"18","author":"Randhawa","year":"2014","journal-title":"Korean J. Physiol. Pharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/S0016-5085(89)80079-4","article-title":"Hapten-induced model of chronic inflammation and ulceration in the rat colon","volume":"96","author":"Morris","year":"1989","journal-title":"Gastroenterology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.amsu.2016.07.019","article-title":"The TNBS-induced colitis animal model: An overview","volume":"11","author":"Antoniou","year":"2016","journal-title":"Ann. Med. Surg. (Lond.)"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1523\/JNEUROSCI.16-03-01066.1996","article-title":"The possible role of hydrogen sulfide as an endogenous neuromodulator","volume":"16","author":"Abe","year":"1996","journal-title":"J. Neurosci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1042\/bj2060267","article-title":"Characterization of the enzymic capacity for cysteine desulphhydration in liver and kidney of the rat","volume":"206","author":"Stipanuk","year":"1982","journal-title":"Biochem. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1096\/fj.02-0211hyp","article-title":"Two\u2019s company, three\u2019s a crowd: Can H2S be the third endogenous gaseous transmitter?","volume":"16","author":"Wang","year":"2002","journal-title":"FASEB J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1089\/ars.2013.5309","article-title":"Production and physiological effects of hydrogen sulfide","volume":"20","author":"Kimura","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1038\/nrd2425","article-title":"Hydrogen sulphide and its therapeutic potential","volume":"6","author":"Szabo","year":"2007","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_15","first-page":"208","article-title":"Potential role of hydrogen sulfide in the pathogenesis of vascular dysfunction in septic shock","volume":"11","author":"Coletta","year":"2013","journal-title":"Curr. Vasc. Pharmacol."},{"key":"ref_16","first-page":"4","article-title":"Hydrogen sulfide (H2S)\u2014the third gas of interest for pharmacologists","volume":"59","author":"Lowicka","year":"2007","journal-title":"Pharmacol. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1038\/sj.bjp.0704858","article-title":"The smooth muscle relaxant effect of hydrogen sulphide in vitro: Evidence for a physiological role to control intestinal contractility","volume":"137","author":"Teague","year":"2002","journal-title":"Br. J. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1089\/ars.2009.2900","article-title":"Physiological and pathophysiological roles of hydrogen sulfide in the gastrointestinal tract","volume":"12","author":"Wallace","year":"2010","journal-title":"Antioxid. Redox Signal."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1111\/j.1748-1716.2010.02152.x","article-title":"Stimulation of duodenal HCO3\u2212 secretion by hydrogen sulphide in rats: Relation to prostaglandins, nitric oxide and sensory neurones","volume":"201","author":"Ise","year":"2011","journal-title":"Acta Physiol. (Oxf.)"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4070","DOI":"10.1096\/fj.07-8669com","article-title":"Hydrogen sulfide enhances ulcer healing in rats","volume":"21","author":"Wallace","year":"2007","journal-title":"FASEB J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/sj.bjp.0707193","article-title":"Enhanced activity of a hydrogen sulphide-releasing derivative of mesalamine (ATB-429) in a mouse model of colitis","volume":"150","author":"Fiorucci","year":"2007","journal-title":"Br. J. Pharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3748\/wjg.v20.i1.6","article-title":"Immunopathology of inflammatory bowel disease","volume":"20","author":"Wallace","year":"2014","journal-title":"World J. Gastroenterol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/nri3399","article-title":"Neutrophil recruitment and function in health and inflammation","volume":"13","author":"Kolaczkowska","year":"2013","journal-title":"Nat. Rev. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1126\/science.1092385","article-title":"Neutrophil extracellular traps kill bacteria","volume":"303","author":"Brinkmann","year":"2004","journal-title":"Science"},{"key":"ref_25","first-page":"651497","article-title":"NETosis","volume":"2013","author":"Mesa","year":"2013","journal-title":"Autoimmune Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"360","DOI":"10.3389\/fimmu.2012.00360","article-title":"Activation of PAD4 in NET formation","volume":"3","author":"Rohrbach","year":"2012","journal-title":"Front. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"302","DOI":"10.3389\/fimmu.2016.00302","article-title":"New Insights into Neutrophil Extracellular Traps: Mechanisms of Formation and Role in Inflammation","volume":"7","author":"Yang","year":"2016","journal-title":"Front. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nm.4294","article-title":"An emerging role for neutrophil extracellular traps in noninfectious disease","volume":"23","author":"Jorch","year":"2017","journal-title":"Nat. Med."},{"key":"ref_29","first-page":"81","article-title":"Neutrophil Extracellular Traps and Its Implications in Inflammation: An Overview","volume":"8","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"8972","DOI":"10.1038\/s41598-017-09337-4","article-title":"CitH3: A reliable blood biomarker for diagnosis and treatment of endotoxic shock","volume":"7","author":"Pan","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1007\/s12016-020-08804-7","article-title":"A Review of Neutrophil Extracellular Traps (NETs) in Disease: Potential Anti-NETs Therapeutics","volume":"61","author":"Mutua","year":"2021","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1136\/annrheumdis-2014-205365","article-title":"Peptidylarginine deiminase inhibition disrupts NET formation and protects against kidney, skin and vascular disease in lupus-prone MRL\/lpr mice","volume":"74","author":"Knight","year":"2015","journal-title":"Ann. Rheum. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.3389\/fimmu.2019.02957","article-title":"Citrullinated Histone H3 as a Therapeutic Target for Endotoxic Shock in Mice","volume":"10","author":"Deng","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.mce.2018.10.013","article-title":"Hydrogen sulfide primes diabetic wound to close through inhibition of NETosis","volume":"480","author":"Yang","year":"2019","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.bbrc.2020.12.059","article-title":"Exogenous hydrogen sulfide inhibits neutrophils extracellular traps formation via the HMGB1\/TLR4\/p-38 MAPK\/ROS axis in hyperhomocysteinemia rats","volume":"537","author":"Zhao","year":"2021","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"L342","DOI":"10.1152\/ajplung.00151.2012","article-title":"HMGB1 promotes neutrophil extracellular trap formation through interactions with Toll-like receptor 4","volume":"304","author":"Tadie","year":"2013","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1097\/MIB.0000000000000113","article-title":"Role of HMGB1 as a suitable biomarker of subclinical intestinal inflammation and mucosal healing in patients with inflammatory bowel disease","volume":"20","author":"Palone","year":"2014","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1172\/JCI11830","article-title":"NF-kappaB: A key role in inflammatory diseases","volume":"107","author":"Tak","year":"2001","journal-title":"J. Clin. Investig."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nri910","article-title":"NF-kappaB regulation in the immune system","volume":"2","author":"Li","year":"2002","journal-title":"Nat. Rev. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1111\/j.1365-2796.2008.01953.x","article-title":"NF-kappaB in inflammatory bowel disease","volume":"263","author":"Atreya","year":"2008","journal-title":"J. Intern. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1038\/s41419-018-0532-y","article-title":"The deubiquitinating enzyme UCHL1 negatively regulates the immunosuppressive capacity and survival of multipotent mesenchymal stromal cells","volume":"9","author":"Gu","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Almasi, N., Torok, S., Dvoracsko, S., Tomboly, C., Csonka, A., Barath, Z., Murlasits, Z., Valkusz, Z., Posa, A., and Varga, C. (2020). Lessons on the Sigma-1 Receptor in TNBS-Induced Rat Colitis: Modulation of the UCHL-1, IL-6 Pathway. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21114046"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1161\/ATVBAHA.107.142505","article-title":"Ubiquitin carboxyl-terminal hydrolase L1, a novel deubiquitinating enzyme in the vasculature, attenuates NF-kappaB activation","volume":"27","author":"Takami","year":"2007","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1007\/s10787-017-0382-8","article-title":"H2S confers colonoprotection against TNBS-induced colitis by HO-1 upregulation in rats","volume":"26","author":"Kupai","year":"2018","journal-title":"Inflammopharmacology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/1523-1747.ep12506462","article-title":"Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker","volume":"78","author":"Bradley","year":"1982","journal-title":"J. Investig. Dermatol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6008","DOI":"10.1093\/emboj\/20.21.6008","article-title":"The vasorelaxant effect of H2S as a novel endogenous gaseous K(ATP) channel opener","volume":"20","author":"Zhao","year":"2001","journal-title":"EMBO J."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2118","DOI":"10.1096\/fj.06-6270fje","article-title":"Hydrogen sulfide is an endogenous modulator of leukocyte-mediated inflammation","volume":"20","author":"Zanardo","year":"2006","journal-title":"FASEB J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1586\/ecp.10.122","article-title":"Therapeutic potential of new hydrogen sulfide-releasing hybrids","volume":"4","author":"Sparatore","year":"2011","journal-title":"Expert Rev. Clin. Pharmacol."},{"key":"ref_49","first-page":"749","article-title":"The effect of hydrogen sulfide-releasing naproxen (ATB-346) versus naproxen on formation of stress-induced gastric lesions, the regulation of systemic inflammation, hypoxia and alterations in gastric microcirculation","volume":"68","author":"Magierowski","year":"2017","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/s40635-020-0296-4","article-title":"Emerging pharmacological tools to control hydrogen sulfide signaling in critical illness","volume":"8","author":"Marutani","year":"2020","journal-title":"Intensive Care Med. Exp."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1111\/j.1476-5381.2009.00611.x","article-title":"Markedly reduced toxicity of a hydrogen sulphide-releasing derivative of naproxen (ATB-346)","volume":"159","author":"Wallace","year":"2010","journal-title":"Br. J. Pharmacol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1111\/bph.14641","article-title":"A proof-of-concept, Phase 2 clinical trial of the gastrointestinal safety of a hydrogen sulfide-releasing anti-inflammatory drug","volume":"177","author":"Wallace","year":"2020","journal-title":"Br. J. Pharmacol."},{"key":"ref_53","first-page":"483","article-title":"Current medical therapy of inflammatory bowel disease","volume":"6","author":"Das","year":"2000","journal-title":"World J. Gastroenterol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1631\/jzus.B1500248","article-title":"Hydrogen sulfide from a NaHS source attenuates dextran sulfate sodium (DSS)-induced inflammation via inhibiting nuclear factor-kappaB","volume":"17","author":"Chen","year":"2016","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1097\/MIB.0000000000000460","article-title":"Neutrophil Extracellular Traps in Ulcerative Colitis: A Proteome Analysis of Intestinal Biopsies","volume":"21","author":"Bennike","year":"2015","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1093\/ecco-jcc\/jjz132","article-title":"Neutrophil Extracellular Traps Induce Intestinal Damage and Thrombotic Tendency in Inflammatory Bowel Disease","volume":"14","author":"Li","year":"2020","journal-title":"J. Crohns Colitis"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1111\/cei.13226","article-title":"Recruitment of activated neutrophils correlates with disease severity in adult Crohn\u2019s disease","volume":"195","author":"Therrien","year":"2019","journal-title":"Clin. Exp. Immunol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/ecco-jcc\/jjy215","article-title":"Neutrophil Extracellular Traps Sustain Inflammatory Signals in Ulcerative Colitis","volume":"13","author":"Dinallo","year":"2019","journal-title":"J. Crohns Colitis"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"106583","DOI":"10.1016\/j.intimp.2020.106583","article-title":"Evaluation of protein arginine deiminase-4 inhibitor in TNBS- induced colitis in mice","volume":"84","author":"Zhang","year":"2020","journal-title":"Int. Immunopharmacol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"G929","DOI":"10.1152\/ajpgi.00435.2010","article-title":"Suppression of colitis in mice by Cl-amidine: A novel peptidylarginine deiminase inhibitor","volume":"300","author":"Chumanevich","year":"2011","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4396","DOI":"10.4049\/jimmunol.1001620","article-title":"N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide, a protein arginine deiminase inhibitor, reduces the severity of murine collagen-induced arthritis","volume":"186","author":"Willis","year":"2011","journal-title":"J. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"25941","DOI":"10.1074\/jbc.M112.375725","article-title":"Anticancer peptidylarginine deiminase (PAD) inhibitors regulate the autophagy flux and the mammalian target of rapamycin complex 1 activity","volume":"287","author":"Wang","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.bbrc.2007.06.065","article-title":"Essential roles of high-mobility group box 1 in the development of murine colitis and colitis-associated cancer","volume":"360","author":"Maeda","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.freeradbiomed.2006.03.021","article-title":"Hydrogen sulfide inhibits nitric oxide production and nuclear factor-kappaB via heme oxygenase-1 expression in RAW264.7 macrophages stimulated with lipopolysaccharide","volume":"41","author":"Oh","year":"2006","journal-title":"Free Radic. Biol. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1146\/annurev-pharmtox-010510-100505","article-title":"Hydrogen sulfide and cell signaling","volume":"51","author":"Li","year":"2011","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"eaal3062","DOI":"10.1126\/sciimmunol.aal3062","article-title":"Citrullination of NF-kappaB p65 promotes its nuclear localization and TLR-induced expression of IL-1beta and TNFalpha","volume":"2","author":"Sun","year":"2017","journal-title":"Sci. Immunol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/23\/12729\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:35:34Z","timestamp":1760168134000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/23\/12729"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,25]]},"references-count":66,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["ijms222312729"],"URL":"https:\/\/doi.org\/10.3390\/ijms222312729","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,25]]}}}