{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:33:02Z","timestamp":1792643582560,"version":"4.5.0"},"reference-count":77,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T00:00:00Z","timestamp":1638230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["P1-0201"],"award-info":[{"award-number":["P1-0201"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["P3-067"],"award-info":[{"award-number":["P3-067"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In parallel to medical treatment of ovarian cancer, methods for the early detection of cancer tumors are being sought. In this contribution, the use of non-invasive static (SLS) and dynamic light scattering (DLS) for the characterization of extracellular nanoparticles (ENPs) in body fluids of advanced serous ovarian cancer (OC) and benign gynecological pathology (BP) patients is demonstrated and critically evaluated. Samples of plasma and ascites (OC patients) or plasma, peritoneal fluid, and peritoneal washing (BP patients) were analyzed. The hydrodynamic radius (Rh) and the radius of gyration (Rg) of ENPs were calculated from the angular dependency of LS intensity for two ENP subpopulations. Rh and Rg of the predominant ENP population of OC patients were in the range 20\u201330 nm (diameter 40\u201360 nm). In thawed samples, larger particles (Rh mostly above 100 nm) were detected as well. The shape parameter \u03c1 of both particle populations was around 1, which is typical for spherical particles with mass concentrated on the rim, as in vesicles. The Rh and Rg of ENPs in BP patients were larger than in OC patients, with \u03c1 \u2248 1.1\u20132, implying a more elongated\/distorted shape. These results show that SLS and DLS are promising methods for the analysis of morphological features of ENPs and have the potential to discriminate between OC and BP patients. However, further development of the methodology is required.<\/jats:p>","DOI":"10.3390\/ijms222312946","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T23:22:28Z","timestamp":1638314548000},"page":"12946","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Application of Dynamic and Static Light Scattering for Size and Shape Characterization of Small Extracellular Nanoparticles in Plasma and Ascites of Ovarian Cancer Patients"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6291-1032","authenticated-orcid":false,"given":"Ksenija","family":"Kogej","sequence":"first","affiliation":[{"name":"Faculty of Chemistry and Chemical Technology, Department of Chemistry and Biochemistry, University of Ljubljana, SI-1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8449-310X","authenticated-orcid":false,"given":"Darja","family":"Bo\u017ei\u010d","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry and Chemical Technology, Department of Chemistry and Biochemistry, University of Ljubljana, SI-1000 Ljubljana, Slovenia"},{"name":"Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, SI-1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7913-7699","authenticated-orcid":false,"given":"Borut","family":"Kobal","sequence":"additional","affiliation":[{"name":"Division of Gynecology and Obstetrics, Department of Gynecology, University Medical Centre Ljubljana, SI-1000 Ljubljana, Slovenia"},{"name":"Faculty of Medicine, Department of Gynecology and Obstetrics, University of Ljubljana, SI-1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maru\u0161a","family":"Herzog","sequence":"additional","affiliation":[{"name":"Division of Gynecology and Obstetrics, Department of Gynecology, University Medical Centre Ljubljana, SI-1000 Ljubljana, Slovenia"},{"name":"Faculty of Medicine, Department of Gynecology and Obstetrics, University of Ljubljana, SI-1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3843-8716","authenticated-orcid":false,"given":"Katarina","family":"\u010cerne","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Pharmacology and Experimental Toxicology, University of Ljubljana, SI-1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3322\/caac.21166","article-title":"Cancer statistics, 2013","volume":"63","author":"Siegel","year":"2013","journal-title":"CA Cancer J. 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