{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T02:13:48Z","timestamp":1772244828787,"version":"3.50.1"},"reference-count":57,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2019YFD1000103"],"award-info":[{"award-number":["2019YFD1000103"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31901993, 31970312, 31970200"],"award-info":[{"award-number":["31901993, 31970312, 31970200"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"he Natural Science Foundations of Anhui Province","award":["1908085MC72"],"award-info":[{"award-number":["1908085MC72"]}]},{"name":"the Key Research and Development Program of Anhui Province","award":["202003a06020011"],"award-info":[{"award-number":["202003a06020011"]}]},{"name":"the Fundamental Research Funds for the Central Universities","award":["JZ2021HGPA0063"],"award-info":[{"award-number":["JZ2021HGPA0063"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Calcium deficiency usually causes accelerated quality deterioration in postharvest fruit, whereas the underlining mechanism is still unclear. Here, we report that calcium deficiency induced the development of bitter pit on the surface of apple peels compared with the healthy appearance in control apples during postharvest storage. Physiological analysis indicates that calcium-deficient peels contained higher levels of superoxide anion (O2\u2022\u2212), malondialdehyde (MDA), total phenol, flavonoid contents and polyphenol oxidase (PPO) activity, and reduced calcium, H2S production, anthocyanin, soluble protein content, and peroxidase (POD) activity compared with those in calcium-sufficient peels. The principal component analysis (PCA) results show that calcium content, ROS, and H2S production were the main factors between calcium-deficient and calcium-sufficient apple peels. Transcriptome data indicated that four calmodulin-like proteins (CMLs), seven AP2\/ERFs, and three bHLHs transcripts were significantly differentially expressed in calcium-deficient apple peels. RT-qPCR and correlation analyses further revealed that CML5 expression was significantly positively correlated with the expression of ERF2\/17, bHLH2, and H2S production related genes. In addition, transcriptional co-activation of CML5 by ERF2 and bHLH2 was demonstrated by apple transient expression assays and dual-luciferase reporter system experiments. Therefore, these findings provide a basis for studying the molecular mechanism of postharvest quality decline in calcium-deficient apples and the potential interaction between Ca2+ and endogenous H2S.<\/jats:p>","DOI":"10.3390\/ijms222313013","type":"journal-article","created":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T02:40:02Z","timestamp":1638412802000},"page":"13013","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Transcriptomics Reveals the ERF2-bHLH2-CML5 Module Responses to H2S and ROS in Postharvest Calcium Deficiency Apples"],"prefix":"10.3390","volume":"22","author":[{"given":"Hong-Ye","family":"Sun","sequence":"first","affiliation":[{"name":"School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai\u2019an 271001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Yong","family":"Qu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sha-Sha","family":"Gou","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Xia","family":"Li","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-Hui","family":"Song","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Qiang","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai\u2019an 271001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6439-7535","authenticated-orcid":false,"given":"Wan-Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Cell Proliferation and Regulation Biology, College of Life Science, Beijing Normal University, Ministry of Education, Beijing 100875, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3980-1262","authenticated-orcid":false,"given":"Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kang-Di","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gai-Fang","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,1]]},"reference":[{"key":"ref_1","first-page":"19","article-title":"Calcium alleviates stress in plants: Insight into regulatory mechanisms","volume":"3","author":"Delian","year":"2014","journal-title":"AgroLife Sci. 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