{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T16:53:46Z","timestamp":1778950426449,"version":"3.51.4"},"reference-count":40,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T00:00:00Z","timestamp":1639699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NANOMED EMJMD","award":["574439-EPP-1-FR-EPPKA1-JMD-MOB"],"award-info":[{"award-number":["574439-EPP-1-FR-EPPKA1-JMD-MOB"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Peripheral artery occlusive disease is an emerging cardiovascular disease characterized by the blockage of blood vessels in the limbs and is associated with dysfunction, gangrene, amputation, and a high mortality risk. Possible treatments involve by-pass surgery using autologous vessel grafts, because of the lack of suitable synthetic small-diameter vascular prosthesis. One to five percent of patients experience vascular graft infection, with a high risk of haemorrhage, spreading of the infection, amputation and even death. In this work, an infection-proof vascular graft prototype was designed and manufactured by electrospinning 12.5% w\/v poly-L-lactic-co-glycolic acid solution in 75% v\/v dichloromethane, 23.8% v\/v dimethylformamide and 1.2% v\/v water, loaded with 0.2% w\/wPLGA. Polymer and tobramycin concentrations were selected after viscosity and surface tension and after HPLC-UV encapsulation efficiency (EE%) evaluation, respectively. The final drug-loaded prototype had an EE% of 95.58% \u00b1 3.14%, with smooth fibres in the nanometer range and good porosity; graft wall thickness was 291 \u00b1 20.82 \u03bcm and its internal diameter was 2.61 \u00b1 0.05 mm. The graft\u2019s antimicrobic activity evaluation through time-kill assays demonstrated a significant and strong antibacterial activity over 5 days against Staphylococcus aureus and Escherichia coli. An indirect cell viability assay on Normal Human Dermal Fibroblasts (NHDF) confirmed the cytocompatibility of the grafts.<\/jats:p>","DOI":"10.3390\/ijms222413557","type":"journal-article","created":{"date-parts":[[2021,12,19]],"date-time":"2021-12-19T20:37:27Z","timestamp":1639946247000},"page":"13557","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Tobramycin Supplemented Small-Diameter Vascular Grafts for Local Antibiotic Delivery: A Preliminary Formulation Study"],"prefix":"10.3390","volume":"22","author":[{"given":"Mariella","family":"Rosalia","sequence":"first","affiliation":[{"name":"Department of Drug Sciences, Pharmaceutical Section, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priusha","family":"Ravipati","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, Pharmaceutical Section, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2016-0135","authenticated-orcid":false,"given":"Pietro","family":"Grisoli","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, Pharmacological Section, University of Pavia, Via Taramelli 16, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5774-9547","authenticated-orcid":false,"given":"Rossella","family":"Dorati","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, Pharmaceutical Section, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5710-0588","authenticated-orcid":false,"given":"Ida","family":"Genta","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, Pharmaceutical Section, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrica","family":"Chiesa","sequence":"additional","affiliation":[{"name":"Department of Surgery, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1958-2998","authenticated-orcid":false,"given":"Giovanna","family":"Bruni","sequence":"additional","affiliation":[{"name":"Consorzio per lo Sviluppo dei Sistemi a Grande Interfase (C.S.G.I.) & Department of Chemistry, Physical Chemistry Section, University of Pavia, Via Taramelli 10, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0034-2815","authenticated-orcid":false,"given":"Bice","family":"Conti","sequence":"additional","affiliation":[{"name":"Department of Drug Sciences, Pharmaceutical Section, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1016\/S0140-6736(13)61249-0","article-title":"Comparison of global estimates of prevalence and risk factors for peripheral artery disease in 2000 and 2010: A systematic review and analysis","volume":"382","author":"Fowkes","year":"2013","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1161\/CIRCRESAHA.116.303849","article-title":"Epidemiology of Peripheral Artery Disease","volume":"116","author":"Criqui","year":"2015","journal-title":"Circ. 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