{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:16:17Z","timestamp":1773281777701,"version":"3.50.1"},"reference-count":23,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,25]],"date-time":"2021-12-25T00:00:00Z","timestamp":1640390400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Federal Ministry of Education and Research","doi-asserted-by":"publisher","award":["BMBF 01 EO 0803"],"award-info":[{"award-number":["BMBF 01 EO 0803"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB1160 \u2013 IMPAT"],"award-info":[{"award-number":["SFB1160 \u2013 IMPAT"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"F\u00f6rdergesellschaft Forschung Tumorbiologie","award":["Grant \u201cLiquid-Biopsy-Initiative\u201d"],"award-info":[{"award-number":["Grant \u201cLiquid-Biopsy-Initiative\u201d"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent from the primary and secondary antibodies and retrieval. Histomorphologic details are preserved, and crossreactivity and loss of signal intensity are not detectable. Combined with a DAB-based hydrophobic masking of metal-labeled primary antibodies, FOLGAS allows the extended use of CyTOF imaging in FFPE sections.<\/jats:p>","DOI":"10.3390\/ijms23010223","type":"journal-article","created":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T01:00:54Z","timestamp":1640566854000},"page":"223","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Multiple Immunostainings with Different Epitope Retrievals\u2014The FOLGAS Protocol"],"prefix":"10.3390","volume":"23","author":[{"given":"Anna","family":"von Schoenfeld","sequence":"first","affiliation":[{"name":"Institute for Surgical Pathology, Medical Center\u2014University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"},{"name":"Clinic for Internal Medicine, Berlin Jewish Hospital, 13347 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8558-0347","authenticated-orcid":false,"given":"Peter","family":"Bronsert","sequence":"additional","affiliation":[{"name":"Institute for Surgical Pathology, Medical Center\u2014University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"},{"name":"Tumorbank Comprehensive Cancer Center Freiburg, University of Freiburg, 79106 Freiburg, Germany"},{"name":"Core Facility for Histopathology and Digital Pathology, Medical Center\u2014University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Poc","sequence":"additional","affiliation":[{"name":"Institute for Surgical Pathology, Medical Center\u2014University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"},{"name":"Klinik Gut St. Moritz, 7500 St. Moritz, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Fuller","sequence":"additional","affiliation":[{"name":"Flow Cytometry Core Facility, Innovation, Methodology and Application Research Theme, Leech Building, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Filby","sequence":"additional","affiliation":[{"name":"Flow Cytometry Core Facility, Innovation, Methodology and Application Research Theme, Leech Building, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Kraft","sequence":"additional","affiliation":[{"name":"Center for Dermatopathology, 79106 Freiburg, 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