{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T16:44:27Z","timestamp":1783961067386,"version":"3.55.0"},"reference-count":49,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T00:00:00Z","timestamp":1640822400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007660","name":"Universiteit Antwerpen","doi-asserted-by":"publisher","award":["33931"],"award-info":[{"award-number":["33931"]}],"id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007660","name":"Universiteit Antwerpen","doi-asserted-by":"publisher","award":["40183"],"award-info":[{"award-number":["40183"]}],"id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Horizon 2020","award":["675527"],"award-info":[{"award-number":["675527"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Acetylsalicylic acid (ASA) is widely used in secondary prevention of cardiovascular (CV) disease, mainly because of its antithrombotic effects. Here, we investigated whether ASA can prevent the progression of vessel wall remodelling, atherosclerosis, and CV complications in apolipoprotein E deficient (ApoE\u2212\/\u2212) mice, a model of stable atherosclerosis, and in ApoE\u2212\/\u2212 mice with a mutation in the fibrillin-1 gene (Fbn1C1039G+\/\u2212), which is a model of elastic fibre fragmentation, accompanied by exacerbated unstable atherosclerosis. Female ApoE\u2212\/\u2212 and ApoE\u2212\/\u2212Fbn1C1039G+\/\u2212 mice were fed a Western diet (WD). At 10 weeks of WD, the mice were randomly divided into four groups, receiving either ASA 5 mg\/kg\/day in the drinking water (ApoE\u2212\/\u2212 (n = 14), ApoE\u2212\/\u2212Fbn1C1039G+\/\u2212 (n = 19)) or plain drinking water (ApoE\u2212\/\u2212 (n = 15), ApoE\u2212\/\u2212Fbn1C1039G+\/\u2212 (n = 21)) for 15 weeks. ApoE\u2212\/\u2212Fbn1C1039G+\/\u2212 mice showed an increased neutrophil\u2013lymphocyte ratio (NLR) compared to ApoE\u2212\/\u2212 mice, and this effect was normalised by ASA. In the proximal ascending aorta wall, ASA-treated ApoE\u2212\/\u2212Fbn1C1039G+\/\u2212 mice showed less p-SMAD2\/3 positive nuclei, a lower collagen percentage and an increased elastin\/collagen ratio, consistent with the values measured in ApoE\u2212\/\u2212 mice. ASA did not affect plaque progression, incidence of myocardial infarction and survival of ApoE\u2212\/\u2212Fbn1C1039G+\/\u2212 mice, but systolic blood pressure, cardiac fibrosis and hypertrophy were reduced. In conclusion, ASA normalises the NLR, passive wall stiffness and cardiac remodelling in ApoE\u2212\/\u2212Fbn1C1039G+\/\u2212 mice to levels observed in ApoE\u2212\/\u2212 mice, indicating additional therapeutic benefits of ASA beyond its classical use.<\/jats:p>","DOI":"10.3390\/ijms23010404","type":"journal-article","created":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T21:41:21Z","timestamp":1640900481000},"page":"404","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Acetylsalicylic Acid Reduces Passive Aortic Wall Stiffness and Cardiovascular Remodelling in a Mouse Model of Advanced Atherosclerosis"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1397-1810","authenticated-orcid":false,"given":"Lynn","family":"Roth","sequence":"first","affiliation":[{"name":"Laboratory of Physiopharmacology, University of Antwerp, 2610 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miche","family":"Rombouts","sequence":"additional","affiliation":[{"name":"Laboratory of Physiopharmacology, University of Antwerp, 2610 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorien M.","family":"Schrijvers","sequence":"additional","affiliation":[{"name":"Laboratory of Physiopharmacology, University of Antwerp, 2610 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Besa","family":"Emini Veseli","sequence":"additional","affiliation":[{"name":"Laboratory of Physiopharmacology, University of Antwerp, 2610 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1967-9343","authenticated-orcid":false,"given":"Wim","family":"Martinet","sequence":"additional","affiliation":[{"name":"Laboratory of Physiopharmacology, University of Antwerp, 2610 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3848-8702","authenticated-orcid":false,"given":"Guido R. Y.","family":"De Meyer","sequence":"additional","affiliation":[{"name":"Laboratory of Physiopharmacology, University of Antwerp, 2610 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vph.2015.11.083","article-title":"Arterial stiffness, atherosclerosis and cardiovascular risk: Pathophysiologic mechanisms and emerging clinical indications","volume":"77","author":"Palombo","year":"2016","journal-title":"Vascul. 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