{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T20:48:14Z","timestamp":1776458894254,"version":"3.51.2"},"reference-count":33,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T00:00:00Z","timestamp":1642118400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1154805"],"award-info":[{"award-number":["APP1154805"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In a quest for new interventions against scabies\u2014a highly significant skin disease of mammals, caused by a parasitic mite Sarcoptes scabiei\u2014we are focusing on finding new intervention targets. RNA interference (RNAi) could be an efficient functional genomics approach to identify such targets. The RNAi pathway is present in S. scabiei and operational in the female adult mite, but other developmental stages have not been assessed. Identifying potential intervention targets in the egg stage is particularly important because current treatments do not kill this latter stage. Here, we established an RNAi tool to silence single-copy genes in S. scabiei eggs. Using sodium hypochlorite pre-treatment, we succeeded in rendering the eggshell permeable to dsRNA without affecting larval hatching. We optimised the treatment of eggs with gene-specific dsRNAs to three single-copy target genes (designated Ss-Cof, Ss-Ddp, and Ss-Nan) which significantly and repeatedly suppressed transcription by ~66.6%, 74.3%, and 84.1%, respectively. Although no phenotypic alterations were detected in dsRNA-treated eggs for Ss-Cof and Ss-Nan, the silencing of Ss-Ddp resulted in a 38% reduction of larval hatching. This RNAi method is expected to provide a useful tool for larger-scale functional genomic investigations for the identification of essential genes as potential drug targets.<\/jats:p>","DOI":"10.3390\/ijms23020873","type":"journal-article","created":{"date-parts":[[2022,1,14]],"date-time":"2022-01-14T03:14:03Z","timestamp":1642130043000},"page":"873","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["An RNA Interference Tool to Silence Genes in Sarcoptes scabiei Eggs"],"prefix":"10.3390","volume":"23","author":[{"given":"Deepani D.","family":"Fernando","sequence":"first","affiliation":[{"name":"Infectious Diseases Program, Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pasi K.","family":"Korhonen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, VIC 3052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4423-1690","authenticated-orcid":false,"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Parkville, VIC 3052, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katja","family":"Fischer","sequence":"additional","affiliation":[{"name":"Infectious Diseases Program, Cell and Molecular Biology Department, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1056\/NEJMcp052784","article-title":"Clinical practices","volume":"354","author":"Chosidow","year":"2006","journal-title":"Scabies. N. Engl. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Romani, L., Koroivueta, J., Steer, A.C., Kama, M., Kaldor, J.M., Wand, H., Hamid, M., and Whitfeld, M.J. (2015). Scabies and Impetigo Prevalence and Risk Factors in Fiji: A National Survey. PLoS Negl. Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003452"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1016\/S1473-3099(17)30483-8","article-title":"The global burden of scabies: A cross-sectional analysis from the Global Burden of Disease Study 2015","volume":"17","author":"Karimkhani","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0304-4017(95)00795-3","article-title":"Sarcoptic mange and production performance of swine: A review of the literature and studies of associations between mite infestation, growth rate and measures of mange severity in growing pigs","volume":"60","author":"Davies","year":"1995","journal-title":"Vet. Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.jaad.2019.11.069","article-title":"How to eliminate scabies parasites from fomites: A high-throughput Ex vivo experimental study","volume":"83","author":"Bernigaud","year":"2020","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1111\/bjd.18517","article-title":"In vitro ovicidal activity of current and under-development scabicides: Which treatments kill scabies eggs?","volume":"182","author":"Bernigaud","year":"2020","journal-title":"Br. J. Dermatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"57","DOI":"10.2217\/17460913.3.1.57","article-title":"Scabies: Molecular perspectives and therapeutic implications in the face of emerging drug resistance","volume":"3","author":"Holt","year":"2008","journal-title":"Futur. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kemphues, K. (2005). Essential genes. WormBook, WormBase.","DOI":"10.1895\/wormbook.1.57.1"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Korhonen, P.K., Gasser, R.B., Ma, G., Wang, T., Stroehlein, A.J., Young, N.D., Ang, C.-S., Fernando, D.D., Lu, H.C., and Taylor, S. (2020). High-quality nuclear genome for Sarcoptes scabiei\u2014A critical resource for a neglected parasite. PLoS Neglected Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008720"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1186\/s13071-017-2226-1","article-title":"Gene silencing by RNA interference in Sarcoptes scabiei: A molecular tool to identify novel therapeutic targets","volume":"10","author":"Fernando","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1186\/s13567-018-0608-9","article-title":"Gene silencing by RNA interference in the ectoparasitic mite, Psoroptes ovis","volume":"49","author":"Marr","year":"2018","journal-title":"Vet. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s00427-007-0132-9","article-title":"Gene silencing in the spider mite Tetranychus urticae: dsRNA and siRNA parental silencing of the Distal-less gene","volume":"217","author":"Khila","year":"2007","journal-title":"Dev. Genes Evol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Mounsey, K., Ho, M.-F., Kelly, A., Willis, C., Pasay, C., Kemp, D.J., McCarthy, J., and Fischer, K. (2010). A Tractable Experimental Model for Study of Human and Animal Scabies. PLoS Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000756"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1038\/35055120","article-title":"Cof\/ADF is required for cell motility during Drosophila ovary development and oogenesis","volume":"3","author":"Chen","year":"2001","journal-title":"Nat. Cell Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"107893","DOI":"10.1016\/j.celrep.2020.107893","article-title":"Cof Loss in Drosophila Muscles Contributes to Muscle Weakness through Defective Sarcomerogenesis during Muscle Growth","volume":"32","author":"BalakrishNan","year":"2020","journal-title":"Cell Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1101\/gad.6.11.2137","article-title":"Ddp, an essential pan-neural gene in Drosophila, encodes a helix-loop-helix protein similar to the hairy gene product","volume":"6","author":"Bier","year":"1992","journal-title":"Genes Dev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"R82","DOI":"10.1152\/ajpregu.00003.2014","article-title":"Altering the sex determination pathway in Drosophila fat body modifies sex-specific stress responses","volume":"307","author":"Argue","year":"2014","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1002\/aja.1001990204","article-title":"Genetics of Nan localization in Drosophila","volume":"199","author":"Wang","year":"1994","journal-title":"Dev. Dyn."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1242\/dev.125.4.679","article-title":"Nan and Pumilio have critical roles in the development and function of Drosophila germline stem cells","volume":"125","author":"Forbes","year":"1998","journal-title":"Development"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Ruiz, N., Abreu, L.A.D., Parizi, L.F., Kim, T.K., Mulenga, A., Braz, G.R.C., Vaz, I.D.S., and Logullo, C. (2015). Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0133949"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ali, M.W., Khan, M.M., Song, F., Wu, L., He, L., Wang, Z., Zhang, Z.-Y., Zhang, H., and Jiang, Y. (2020). RNA Interference-Based Silencing of the Chitin Synthase 1 Gene for Reproductive and Developmental Disruptions in Panonychus citri. Insects, 11.","DOI":"10.3390\/insects11110786"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1472-6750-10-1","article-title":"Functional genomics tool: Gene silencing in Ixodes scapularis eggs and nymphs by electroporated dsRNA","volume":"10","author":"Karim","year":"2010","journal-title":"BMC Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.mcp.2015.07.008","article-title":"Gene silencing by RNA interference in the house dust mite, Dermatophagoides pteronyssinus","volume":"29","author":"Marr","year":"2015","journal-title":"Mol. Cell. Probes"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Suzuki, T., Nunes, M.A., Espa\u00f1a, M.U., Namin, H.H., Jin, P., Bensoussan, N., Zhurov, V., Rahman, T., De Clercq, R., and Hilson, P. (2017). RNAi-based reverse genetics in the chelicerate model Tetranychus urticae: A comparative analysis of five methods for gene silencing. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0180654"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s10493-014-9772-4","article-title":"Oral delivery of double-stranded RNA induces prolonged and systemic gene knockdown in Metaseiulus occidentalis only after feeding on Tetranychus urticae","volume":"63","author":"Wu","year":"2014","journal-title":"Exp. Appl. Acarol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"107829","DOI":"10.1016\/j.exppara.2019.107829","article-title":"Molecular cloning, expression and impact of ribosomal protein S-27 silencing in Haemaphysalis longicornis (Acari: Ixodidae)","volume":"209","author":"Rahman","year":"2020","journal-title":"Exp. Parasitol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1051\/parasite\/2019038","article-title":"Molecular cloning and transcriptional and functional analysis of glycogen synthase kinase-3\u03b2 in Haemaphysalis longicornis (Acari, Ixodidae)","volume":"26","author":"Rahman","year":"2019","journal-title":"Parasite"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.pt.2007.07.002","article-title":"RNA interference for the study and genetic manipulation of ticks","volume":"23","author":"Kocan","year":"2007","journal-title":"Trends Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s13071-020-04562-9","article-title":"RNAi gene knockdown in the poultry red mite, Dermanyssus gallinae (De Geer 1778), a tool for functional genomics","volume":"14","author":"Chen","year":"2021","journal-title":"Parasites Vectors"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1146\/annurev.en.34.010189.001035","article-title":"Biology, Host Relations, and Epidemiology of Sarcoptes Scabiei","volume":"34","author":"Arlian","year":"1989","journal-title":"Annu. Rev. \u00c8ntomol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2178","DOI":"10.1101\/gr.1224503","article-title":"Identification of ortholog groups for eukaryotic genomes","volume":"13","author":"Li","year":"2003","journal-title":"Genome Res"},{"key":"ref_32","first-page":"365","article-title":"Primer3 on the WWW for general users and for biologist programmers","volume":"132","author":"Rozen","year":"2000","journal-title":"Methods Mol. Biol."},{"key":"ref_33","first-page":"1","article-title":"Quantitative PCR-based genome size estimation of the astigmatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus","volume":"5","author":"Willis","year":"2012","journal-title":"Parasites Vectors"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/2\/873\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:39:42Z","timestamp":1760362782000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/2\/873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,14]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,1]]}},"alternative-id":["ijms23020873"],"URL":"https:\/\/doi.org\/10.3390\/ijms23020873","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,14]]}}}