{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T20:39:24Z","timestamp":1770064764298,"version":"3.49.0"},"reference-count":31,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Baden-W\u00fcrttemberg Stiftung (Michael Platten)","award":["BWST_ISF2018-046"],"award-info":[{"award-number":["BWST_ISF2018-046"]}]},{"name":"Else Kr\u00f6ner-Fresenius Foundation (EKMS) and Deutsche Forschungsgemeinschaft, (Lukas Bunse)","award":["SFB 1389\u2013B03"],"award-info":[{"award-number":["SFB 1389\u2013B03"]}]},{"name":"The CMVPepVac Study Scientific Committee designed the study, reviewed the study data and statistical analysis, and wrote the report. All authors had full access to the study data, decided to submit the report for publication, assume responsibility for the","award":["SFB 1389\u2013B03"],"award-info":[{"award-number":["SFB 1389\u2013B03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>After solid-organ transplantation, reactivation of the cytomegalovirus (CMV) is often observed in seronegative patients and associated with a high risk of disease and mortality. CMV-specific T cells can prevent CMV reactivation. In a phase 1 trial, CMV-seronegative patients with end-stage renal disease listed for kidney transplantation were subjected to CMV phosphoprotein 65 (CMVpp65) peptide vaccination and further investigated for T-cell responses. To this end, CMV-specific CD8+ T cells were characterized by bulk T-cell-receptor (TCR) repertoire sequencing and combined single-cell RNA and TCR sequencing. In patients mounting an immune response to the vaccine, a common SYE(N)E TCR motif known to bind CMVpp65 was detected. CMV-peptide-vaccination-responder patients had TCR features distinct from those of non-responders. In a non-responder patient, a monoclonal inflammatory T-cell response was detected upon CMV reactivation. The identification of vaccine-induced CMV-reactive TCRs motifs might facilitate the development of cellular therapies for patients wait-listed for kidney transplantation.<\/jats:p>","DOI":"10.3390\/ijms23031029","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T08:09:20Z","timestamp":1642493360000},"page":"1029","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Common T-Cell-Receptor Motifs and Features in Patients with Cytomegalovirus (CMV)-Seronegative End-Stage Renal Disease Receiving a Peptide Vaccination against CMV"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4490-7574","authenticated-orcid":false,"given":"Lukas","family":"Bunse","sequence":"first","affiliation":[{"name":"DKTK CCU Neuroimmunology and Brain Tumor Immunology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany"},{"name":"Department of Neurology, MCTN, Medical Faculty Mannheim, Heidelberg University, 69117 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Sommerer","sequence":"additional","affiliation":[{"name":"Division of Nephrology, University Hospital Heidelberg, Renal Clinic Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chin Leng","family":"Tan","sequence":"additional","affiliation":[{"name":"DKTK CCU Neuroimmunology and Brain Tumor Immunology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany"},{"name":"Faculty of Biosciences, Heidelberg University, 69117 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7699-7212","authenticated-orcid":false,"given":"Felix","family":"Korell","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine V, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine V, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"H\u00fcckelhoven-Krauss","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine V, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Neuber","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine V, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Mertens","sequence":"additional","affiliation":[{"name":"Institute of Virology, University of Ulm, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4746-887X","authenticated-orcid":false,"given":"Michael","family":"Platten","sequence":"additional","affiliation":[{"name":"DKTK CCU Neuroimmunology and Brain Tumor Immunology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany"},{"name":"Department of Neurology, MCTN, Medical Faculty Mannheim, Heidelberg University, 69117 Heidelberg, Germany"},{"name":"Immune Monitoring Unit, DKFZ and National Center for Tumor Diseases, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward W.","family":"Green","sequence":"additional","affiliation":[{"name":"DKTK CCU Neuroimmunology and Brain Tumor Immunology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Zeier","sequence":"additional","affiliation":[{"name":"Division of Nephrology, University Hospital Heidelberg, Renal Clinic Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1579-1509","authenticated-orcid":false,"given":"Michael","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine V, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1053\/j.ajkd.2011.04.010","article-title":"Human Cytomegalovirus and Kidney Transplantation: A Clinician\u2019s Update","volume":"58","author":"Peeters","year":"2011","journal-title":"Am. 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