{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T07:15:55Z","timestamp":1782371755710,"version":"3.54.5"},"reference-count":36,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T00:00:00Z","timestamp":1643241600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The ability of Pluronic F127 (PF127) conjugated with tetrapeptide Gly-Arg-Gly-Asp (GRGD) as a sequence of Arg-Gly-Asp (RGD) peptide to form the investigated potential hydrogel (hereafter referred to as 3DG bioformer (3BE)) to produce spheroid, biocompatibility, and cell invasion ability, was assessed in this study. The fibroblast cell line (NIH 3T3), osteoblast cell line (MG-63), and human breast cancer cell line (MCF-7) were cultured in the 3BE hydrogel and commercial product (Matrigel) for comparison. The morphology of spheroid formation was evaluated via optical microscopy. The cell viability was observed through cell counting Kit-8 assay, and cell invasion was investigated via Boyden chamber assay. Analytical results indicated that 3BE exhibited lower spheroid formation than Matrigel. However, the 3BE appeared biocompatible to NIH 3T3, MG-63, and MCF-7 cells. Moreover, cell invasion ability and cell survival rate after invasion through the 3BE was displayed to be comparable to Matrigel. Thus, these findings demonstrate that the 3BE hydrogel has a great potential as an alternative to a three-dimensional cell culture for drug screening applications.<\/jats:p>","DOI":"10.3390\/ijms23031488","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T22:00:56Z","timestamp":1643320856000},"page":"1488","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["An Innovative Customized Biomimetic Hydrogel for Drug Screening Application Potential: Biocompatibility and Cell Invasion Ability"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1786-6305","authenticated-orcid":false,"given":"Keng-Liang","family":"Ou","sequence":"first","affiliation":[{"name":"Department of Dentistry, Taipei Medical University-Shuang Ho Hospital, New Taipei City 235, Taiwan"},{"name":"Taiwan Society of Blood Biomaterials, New Taipei City 221, Taiwan"},{"name":"Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Osaka Medical College, Osaka 569-8686, Japan"},{"name":"Department of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung 203, Taiwan"},{"name":"Biomedical Technology R&D Center, China Medical University, Taichung 404, Taiwan"},{"name":"3D Global Biotech Inc. (Spin-Off Company from Taipei Medical University), New Taipei City 221, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiung-Fang","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan"},{"name":"Department of Dentistry, Taipei Medical University Hospital, Taipei 110, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Chien","family":"Lan","sequence":"additional","affiliation":[{"name":"Department of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung 203, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai-Hung","family":"Huang","sequence":"additional","affiliation":[{"name":"Biomedical Technology R&D Center, China Medical University, Taichung 404, Taiwan"},{"name":"Graduate Institute of Dental Science, College of Dentistry, China Medical University, Taichung 404, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsu-An","family":"Pan","sequence":"additional","affiliation":[{"name":"Biomedical Technology R&D Center, China Medical University, Taichung 404, Taiwan"},{"name":"3D Global Biotech Inc. (Spin-Off Company from Taipei Medical University), New Taipei City 221, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4209-3551","authenticated-orcid":false,"given":"Yung-Kang","family":"Shen","sequence":"additional","affiliation":[{"name":"School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8816-7187","authenticated-orcid":false,"given":"Takashi","family":"Saito","sequence":"additional","affiliation":[{"name":"Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Osaka Medical College, Osaka 569-8686, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Yu","family":"Tsai","sequence":"additional","affiliation":[{"name":"Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Osaka Medical College, Osaka 569-8686, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung-Chieh","family":"Cho","sequence":"additional","affiliation":[{"name":"Biomedical Technology R&D Center, China Medical University, Taichung 404, Taiwan"},{"name":"School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo-Sheng","family":"Hung","sequence":"additional","affiliation":[{"name":"Graduate Institute of Injury Prevention and Control, College of Public Health, Taipei Medical University, Taipei 110, Taiwan"},{"name":"Department of Neurosurgery, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Hua","family":"Chou","sequence":"additional","affiliation":[{"name":"School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan"},{"name":"Department of Dentistry, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"13405","DOI":"10.1038\/s41598-019-49775-w","article-title":"Clinical implications of drug-screening assay for recurrent metastatic hormone receptor-positive, human epidermal receptor 2-negative breast cancer using conditionally reprogrammed cells","volume":"9","author":"Mimoto","year":"2019","journal-title":"Sci. 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