{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:07:00Z","timestamp":1790190420114,"version":"4.1.0"},"reference-count":60,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T00:00:00Z","timestamp":1643760000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["14-35-00005"],"award-info":[{"award-number":["14-35-00005"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Type 1 diabetes (T1D) leads to ischemic heart disease and diabetic cardiomyopathy. We tested the hypothesis that T1D differently affects the contractile function of the left and right ventricular free walls (LV, RV) and the interventricular septum (IS) using a rat model of alloxan-induced T1D. Single-myocyte mechanics and cytosolic Ca2+ concentration transients were studied on cardiomyocytes (CM) from LV, RV, and IS in the absence and presence of mechanical load. In addition, we analyzed the phosphorylation level of sarcomeric proteins and the characteristics of the actin-myosin interaction. T1D similarly affected the characteristics of actin-myosin interaction in all studied regions, decreasing the sliding velocity of native thin filaments over myosin in an in vitro motility assay and its Ca2+ sensitivity. A decrease in the thin-filament velocity was associated with increased expression of \u03b2-myosin heavy-chain isoform. However, changes in the mechanical function of single ventricular CM induced by T1D were different. T1D depressed the contractility of CM from LV and RV; it decreased the auxotonic tension amplitude and the slope of the active tension\u2013length relationship. Nevertheless, the contractile function of CM from IS was principally preserved.<\/jats:p>","DOI":"10.3390\/ijms23031719","type":"journal-article","created":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T05:42:33Z","timestamp":1643866953000},"page":"1719","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2966-8437","authenticated-orcid":false,"given":"Anastasia","family":"Khokhlova","sequence":"first","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"},{"name":"Institute of Physics and Technology, Ural Federal University, Mira 19, 620002 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Myachina","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2785-2655","authenticated-orcid":false,"given":"Denis","family":"Volzhaninov","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xenia","family":"Butova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Kochurova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9554-5756","authenticated-orcid":false,"given":"Valentina","family":"Berg","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Gette","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1766-8608","authenticated-orcid":false,"given":"Gleb","family":"Moroz","sequence":"additional","affiliation":[{"name":"Institute of Natural Sciences and Mathematics, Ural Federal University, Mira 19, 620002 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0927-4062","authenticated-orcid":false,"given":"Svetlana","family":"Klinova","sequence":"additional","affiliation":[{"name":"Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, Popova 30, 620014 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilzira","family":"Minigalieva","sequence":"additional","affiliation":[{"name":"Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, Popova 30, 620014 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Solovyova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"},{"name":"Institute of Physics and Technology, Ural Federal University, Mira 19, 620002 Yekaterinburg, Russia"},{"name":"Institute of Natural Sciences and Mathematics, Ural Federal University, Mira 19, 620002 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Danilova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ksenia","family":"Sokolova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2976-2108","authenticated-orcid":false,"given":"Galina","family":"Kopylova","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6660-4673","authenticated-orcid":false,"given":"Daniil","family":"Shchepkin","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Physiology, Russian Academy of Sciences, Pervomajskaya 106, 620049 Yekaterinburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0140-6736(18)31506-X","article-title":"Excess mortality and cardiovascular disease in young adults with type 1 diabetes in relation to age at onset: A nationwide, register-based cohort study","volume":"392","author":"Rawshani","year":"2018","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1161\/CIRCULATIONAHA.118.038137","article-title":"Excess Cardiovascular Risk in Type 1 Diabetes Mellitus: Role for a Dysfunctional Immune Response?","volume":"139","author":"Petrie","year":"2019","journal-title":"Circulation"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S100","DOI":"10.1111\/j.1464-5491.1991.tb02167.x","article-title":"Risk factors of cardiovascular death in diabetic patients","volume":"8","author":"Janeczko","year":"1991","journal-title":"Diabet. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1111\/eci.12673","article-title":"Diabetic cardiomyopathy: A clinical entity or a cluster of molecular heart changes?","volume":"46","author":"Mizamtsidi","year":"2016","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1042\/CS20160491","article-title":"Are targeted therapies for diabetic cardiomyopathy on the horizon?","volume":"131","author":"Tate","year":"2017","journal-title":"Clin. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"124","DOI":"10.3389\/fphys.2020.00124","article-title":"Defining the progression of diabetic cardiomyopathy in a mouse model of type 1 diabetes","volume":"11","author":"Huynh","year":"2020","journal-title":"Front. Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"H2096","DOI":"10.1152\/ajpheart.00452.2009","article-title":"Type 1 diabetic cardiomyopathy in the Akita (Ins2 WT\/C96Y) mouse model is characterized by lipotoxicity and diastolic dysfunction with preserved systolic function","volume":"297","author":"Basu","year":"2009","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1161\/CIRCRESAHA.110.223545","article-title":"Oxidative stress and diabetic complications","volume":"107","author":"Giacco","year":"2010","journal-title":"Circ. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.jacc.2017.11.019","article-title":"Implications of underlying mechanisms for the recognition and management of diabetic cardiomyopathy","volume":"71","author":"Marwick","year":"2018","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"H1514","DOI":"10.1152\/ajpheart.1991.261.5.H1514","article-title":"Significant left ventricular contribution to right ventricular systolic function","volume":"261","author":"Damiano","year":"1991","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1113\/jphysiol.2005.101428","article-title":"Comparison of contraction and calcium handling between right and left ventricular myocytes from adult mouse heart: A role for repolarization waveform","volume":"571","author":"Kondo","year":"2006","journal-title":"J. Physiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"14","DOI":"10.15420\/aer.2016.8.2","article-title":"Differences in left versus right ventricular electrophysiological properties in cardiac dysfunction and arrhythmogenesis","volume":"5","author":"Molina","year":"2016","journal-title":"Arrhythmia Electrophysiol. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s00439-006-0193-2","article-title":"Differential expression of the cardiac ryanodine receptor in normal and arrhythmogenic right ventricular cardiomyopathy canine hearts","volume":"120","author":"Meurs","year":"2006","journal-title":"Hum. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1002\/pmic.201100604","article-title":"Proteomic analysis of right and left cardiac ventricles under aerobic conditions and after ischemia\/reperfusion","volume":"12","author":"Cadete","year":"2012","journal-title":"Proteomics"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1007\/s00424-011-1024-4","article-title":"Interventricular differences in myofilament function in experimental congestive heart failure","volume":"462","author":"Belin","year":"2011","journal-title":"Pfl\u00fcgers Arch. Eur. J. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"B\u00f3di, B., Kov\u00e1cs, \u00c1., Guly\u00e1s, H., M\u00e1rtha, L., T\u00f3th, A., M\u00e1ty\u00e1s, C., Barta, B.A., Ol\u00e1h, A., Merkely, B., and Radovits, T. (2021). Long-Term PDE-5A Inhibition Improves Myofilament Function in Left and Right Ventricular Cardiomyocytes through Partially Different Mechanisms in Diabetic Rat Hearts. Antioxidants, 10.","DOI":"10.3390\/antiox10111776"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1089\/ars.2014.6139","article-title":"Specific mechanisms underlying right heart failure: The missing upregulation of superoxide dismutase-2 and its decisive role in antioxidative defense","volume":"23","author":"Schreckenberg","year":"2015","journal-title":"Antioxid. Redox Signal."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e2108484118","DOI":"10.1073\/pnas.2108484118","article-title":"Mechanoelectric coupling and arrhythmogenesis in cardiomyocytes contracting under mechanical afterload in a 3D viscoelastic hydrogel","volume":"118","author":"Hegyi","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"292","DOI":"10.3389\/fphys.2012.00292","article-title":"Advanced glycation end product cross-link breaker attenuates diabetes-induced cardiac dysfunction by improving sarcoplasmic reticulum calcium handling","volume":"3","author":"Kranstuber","year":"2012","journal-title":"Front. Physiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"537","DOI":"10.33549\/physiolres.930111","article-title":"The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas","volume":"50","author":"Szkudelski","year":"2001","journal-title":"Physiol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1002\/ar.23052","article-title":"Morphological restructuring of myocardium during the early phase of experimental diabetes mellitus","volume":"298","author":"Danilova","year":"2015","journal-title":"Anat. Rec."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"239","DOI":"10.4239\/wjd.v9.i12.239","article-title":"Pathological changes in the cellular structures of retina and choroidea in the early stages of alloxan-induced diabetes","volume":"9","author":"Danilova","year":"2018","journal-title":"World J. Diabetes"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"717","DOI":"10.33549\/physiolres.933270","article-title":"Electrocardiography in rats: A comparison to human","volume":"65","author":"Konopelski","year":"2016","journal-title":"Physiol. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/eci.12583","article-title":"The TyG index may predict the development of cardiovascular events","volume":"46","year":"2016","journal-title":"Eur. J. Clin. Investig."},{"key":"ref_25","unstructured":"Kiernan, J. (2021, December 05). Sirius Red Staining Protocol for Collagen. Available online: http:\/\/www.ihcworld.com\/_protocols\/special_stains\/sirius_red.htm."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"101189","DOI":"10.1016\/j.mex.2020.101189","article-title":"A combined Langendorff-injection technique for simultaneous isolation of single cardiomyocytes from atria and ventricles of the rat heart","volume":"8","author":"Butova","year":"2021","journal-title":"MethodsX"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1134\/S0006350919050178","article-title":"Development and Program Implementation of an Algorithm to Estimate the Mean Sarcomere Length of a Cardiomyocyte","volume":"64","author":"Myachina","year":"2019","journal-title":"Biophysics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"171","DOI":"10.3389\/fphys.2020.00171","article-title":"The effects of mechanical preload on transmural differences in mechano-calcium-electric feedback in single cardiomyocytes: Experiments and mathematical models","volume":"11","author":"Khokhlova","year":"2020","journal-title":"Front. Physiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Volzhaninov, D., and Khokhlova, A. (2019, January 25\u221226). Parallel Control of Two Digital Micromanipulators for Biomechanical Experiments Using LabVIEW. Proceedings of the 2019 Ural Symposium on Biomedical Engineering, Radioelectronics and Information Technology (USBEREIT), Yekaterinburg, Russia.","DOI":"10.1109\/USBEREIT.2019.8736655"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"H196","DOI":"10.1152\/ajpheart.00948.2003","article-title":"Single cell mechanics of rat cardiomyocytes under isometric, unloaded, and physiologically loaded conditions","volume":"287","author":"Nishimura","year":"2004","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_31","first-page":"e51823","article-title":"Analysis of tubular membrane networks in cardiac myocytes from atria and ventricles","volume":"92","author":"Wagner","year":"2014","journal-title":"J. Vis. Exp. JoVE"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0076-6879(82)85009-X","article-title":"[7] Preparation of myosin and its subfragments from rabbit skeletal muscle","volume":"85","author":"Margossian","year":"1982","journal-title":"Methods in Enzymology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"H1048","DOI":"10.1152\/ajpheart.1998.274.3.H1048","article-title":"Electrophoretic separation and quantitation of cardiac myosin heavy chain isoforms in eight mammalian species","volume":"274","author":"Reiser","year":"1998","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/0076-6879(82)85020-9","article-title":"[18] Purification of muscle actin","volume":"85","author":"Pardee","year":"1982","journal-title":"Methods Enzymol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1021\/acs.biochem.6b00994","article-title":"Structural and functional effects of cardiomyopathy-causing mutations in the troponin T-binding region of cardiac tropomyosin","volume":"56","author":"Matyushenko","year":"2017","journal-title":"Biochemistry"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1529\/biophysj.106.081117","article-title":"Automatic detection of single fluorophores in live cells","volume":"92","author":"Mashanov","year":"2007","journal-title":"Biophys. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"815","DOI":"10.3389\/fphys.2020.00815","article-title":"Large-Scale Contractility Measurements Reveal Large Atrioventricular and Subtle Interventricular Differences in Cultured Unloaded Rat Cardiomyocytes","volume":"11","author":"Nollet","year":"2020","journal-title":"Front. Physiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"H88","DOI":"10.1152\/ajpheart.01372.2005","article-title":"Mechanistic basis of differences in Ca2+-handling properties of sarcoplasmic reticulum in right and left ventricles of normal rat myocardium","volume":"291","author":"Sathish","year":"2006","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.3389\/fphys.2018.01799","article-title":"Review on chamber-specific differences in right and left heart reactive oxygen species handling","volume":"9","author":"Kutsche","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1161\/CIRCRESAHA.116.300561","article-title":"Cardiac MyBP-C regulates the rate and force of contraction in mammalian myocardium","volume":"116","author":"Moss","year":"2015","journal-title":"Circ. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0002-9440(10)63647-4","article-title":"ASK1 associates with troponin T and induces troponin T phosphorylation and contractile dysfunction in cardiomyocytes","volume":"163","author":"He","year":"2003","journal-title":"Am. J. Pathol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s00424-011-0964-z","article-title":"Assessment of contractility in intact ventricular cardiomyocytes using the dimensionless \u2018Frank\u2013Starling Gain\u2019index","volume":"462","author":"Bollensdorff","year":"2011","journal-title":"Pfl\u00fcgers Arch. Eur. J. Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"H946","DOI":"10.1152\/ajpheart.00822.2012","article-title":"Intraventricular and interventricular cellular heterogeneity of inotropic responses to \u03b11-adrenergic stimulation","volume":"304","author":"Chu","year":"2013","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Chen, X., Qin, M., Jiang, W., Zhang, Y., and Liu, X. (2017). Electrophysiological characteristics of pressure overload-induced cardiac hypertrophy and its influence on ventricular arrhythmias. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0183671"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Schl\u00fcter, K.-D. (2016). Ways to study the biology of cardiomyocytes. Cardiomyocytes\u2013Active Players in Cardiac Disease, Springer.","DOI":"10.1007\/978-3-319-31251-4"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1161\/01.RES.59.5.556","article-title":"Faster time to peak tension and velocity of shortening in right versus left ventricular trabeculae and papillary muscles of dogs","volume":"59","author":"Rouleau","year":"1986","journal-title":"Circ. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biochi.2020.03.013","article-title":"Unique functional properties of slow skeletal muscle tropomyosin","volume":"174","author":"Matyushenko","year":"2020","journal-title":"Biochimie"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1161\/01.RES.0000174429.00987.17","article-title":"Differences between left and right ventricular chamber geometry affect cardiac vulnerability to electric shocks","volume":"97","author":"Rodriguez","year":"2005","journal-title":"Circ. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"H2013","DOI":"10.1152\/ajpheart.00167.2006","article-title":"Contrasting inotropic responses to \u03b11-adrenergic receptor stimulation in left versus right ventricular myocardium","volume":"291","author":"Wang","year":"2006","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"489","DOI":"10.33549\/physiolres.932467","article-title":"Morphology and contractility of cardiac myocytes in early stages of streptozotocin-induced diabetes mellitus in rats","volume":"62","author":"Cagalinec","year":"2013","journal-title":"Physiol. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"13446","DOI":"10.1074\/jbc.M113.455444","article-title":"Myosin regulatory light chain (RLC) phosphorylation change as a modulator of cardiac muscle contraction in disease","volume":"288","author":"Toepfer","year":"2013","journal-title":"J. Biol. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"16918","DOI":"10.1073\/pnas.0607069103","article-title":"Cardiac myosin binding protein C phosphorylation is cardioprotective","volume":"103","author":"Sadayappan","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/s10974-020-09593-4","article-title":"Acidosis modifies effects of phosphorylated tropomyosin on the actin-myosin interaction in the myocardium","volume":"42","author":"Kopylova","year":"2021","journal-title":"J. Muscle Res. Cell Motil."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1161\/01.HYP.0000104720.76179.18","article-title":"Different growth factor activation in the right and left ventricles in experimental volume overload","volume":"43","author":"Modesti","year":"2004","journal-title":"Hypertension"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"17136","DOI":"10.1038\/s41598-021-96618-8","article-title":"Right versus left ventricular remodeling in heart failure due to chronic volume overload: Pressure-volume and proteome analysis","volume":"11","author":"Havlenova","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1016\/j.bbamem.2013.07.011","article-title":"The physiological role of cardiac cytoskeleton and its alterations in heart failure","volume":"1838","author":"Sequeira","year":"2014","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.cardiores.2003.12.031","article-title":"Reduced synchrony of Ca2+ release with loss of T-tubules\u2014a comparison to Ca2+ release in human failing cardiomyocytes","volume":"62","author":"Louch","year":"2004","journal-title":"Cardiovasc. Res."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Gadeberg, H.C., Bond, R.C., Kong, C.H., Chanoit, G.P., Ascione, R., Cannell, M.B., and James, A.F. (2016). Heterogeneity of T-tubules in pig hearts. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0156862"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.jacc.2010.06.058","article-title":"Oxidation of myofibrillar proteins in human heart failure","volume":"57","author":"Canton","year":"2011","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"100437","DOI":"10.1016\/j.ijcha.2019.100437","article-title":"Impact of oxidative posttranslational modifications of SERCA2 on heart failure exacerbation in young patients with non-ischemic cardiomyopathy: A pilot study","volume":"26","author":"Toya","year":"2020","journal-title":"IJC Heart Vasc."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/3\/1719\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:13:03Z","timestamp":1760134383000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/3\/1719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,2]]},"references-count":60,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["ijms23031719"],"URL":"https:\/\/doi.org\/10.3390\/ijms23031719","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,2]]}}}