{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:59:14Z","timestamp":1787727554537,"version":"build-2784847793"},"reference-count":58,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T00:00:00Z","timestamp":1645056000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["Z3-9261"],"award-info":[{"award-number":["Z3-9261"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["J7-8276"],"award-info":[{"award-number":["J7-8276"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["J3-3153"],"award-info":[{"award-number":["J3-3153"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["P3-0314"],"award-info":[{"award-number":["P3-0314"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Monocytes are known to be implicated in the pathogenesis of systemic sclerosis (SSc), as they exert prominent migratory, adhesive, and chemotactic properties. The aim of our study was to characterize the surface expression of adhesion\/chemotactic molecules (CD62L, CD11b, CCR2, CCR5) on the SSc monocytes and determine correlations with the clinical presentation of SSc. We included 38 SSc patients and 36 healthy age-and sex-matched controls. Isolated monocytes, as well as in vitro serum-treated monocytes, were analyzed by flow cytometry; additionally, soluble CD62L was measured in serum. We found increased soluble CD62L in the SSc serum samples and increased CD62L on the surface of the SSc monocytes in the in the same set of patients. Among samples with determined SSc-specific autoantibodies, the surface CD62L was the lowest in patients positive for anti-PM\/Scl autoantibodies and the highest in patients with anti-topoisomerase I autoantibodies (ATA). The treatment of isolated healthy monocytes with ATA-positive SSc serum resulted in increased surface CD62L expression. Moreover, surface CCR5 was reduced on the monocytes from SSc patients with interstitial lung disease but also, along with CCR2, negatively correlated with the use of analgesics\/anti-inflammatory drugs and immunosuppressants. In conclusion, increased CD62L on SSc monocytes, particularly in ATA-positive patients, provides new insights into the pathogenesis of SSc and suggests CD62L as a potential therapeutic target.<\/jats:p>","DOI":"10.3390\/ijms23042233","type":"journal-article","created":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T20:25:45Z","timestamp":1645129545000},"page":"2233","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Increased L-Selectin on Monocytes Is Linked to the Autoantibody Profile in Systemic Sclerosis"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7341-8673","authenticated-orcid":false,"given":"Ne\u017ea","family":"Brezovec","sequence":"first","affiliation":[{"name":"Department of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia"},{"name":"Faculty of Pharmacy, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Perdan-Pirkmajer","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia"},{"name":"Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4749-594X","authenticated-orcid":false,"given":"Tadeja","family":"Kuret","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bla\u017e","family":"Burja","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia"},{"name":"Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sne\u017ena","family":"Sodin-\u0160emrl","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia"},{"name":"Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, 6000 Koper, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa\u0161a","family":"\u010cu\u010dnik","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia"},{"name":"Faculty of Pharmacy, University of Ljubljana, 1000 Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Lakota","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia"},{"name":"Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, 6000 Koper, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15002","DOI":"10.1038\/nrdp.2015.2","article-title":"Systemic sclerosis","volume":"1","author":"Allanore","year":"2015","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"530","DOI":"10.12968\/hmed.2019.80.9.530","article-title":"Systemic sclerosis","volume":"80","author":"Hughes","year":"2019","journal-title":"Br. J. Hosp. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1038\/s41584-019-0212-z","article-title":"Involvement of the myeloid cell compartment in fibrogenesis and systemic sclerosis","volume":"15","author":"Kania","year":"2019","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1726","DOI":"10.3389\/fimmu.2018.01726","article-title":"Monocyte Subsets: Phenotypes and Function in Tuberculosis Infection","volume":"9","author":"Sampath","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1007\/s10067-017-3597-6","article-title":"CD16-positive circulating monocytes and fibrotic manifestations of systemic sclerosis","volume":"36","author":"Lescoat","year":"2017","journal-title":"Clin. Rheumatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S2213-2600(18)30508-3","article-title":"Increased monocyte count as a cellular biomarker for poor outcomes in fibrotic diseases: A retrospective, multicentre cohort study","volume":"7","author":"Scott","year":"2019","journal-title":"Lancet Respir. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s00011-017-1106-7","article-title":"Increased frequencies of circulating CXCL10-, CXCL8- and CCL4-producing monocytes and Siglec-3-expressing myeloid dendritic cells in systemic sclerosis patients","volume":"67","author":"Carvalheiro","year":"2018","journal-title":"Inflamm. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1007\/s10067-009-1124-0","article-title":"Altered adhesion molecules expression on peripheral blood mononuclear cells from patients with systemic sclerosis and clinical correlations","volume":"28","author":"Sawaya","year":"2009","journal-title":"Clin. Rheumatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1755-1536-4-15","article-title":"Altered monocyte and fibrocyte phenotype and function in scleroderma interstitial lung disease: Reversal by caveolin-1 scaffolding domain peptide","volume":"4","author":"Tourkina","year":"2011","journal-title":"Fibrogenesis Tissue Repair"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1084\/jem.20162152","article-title":"Monocyte-derived alveolar macrophages drive lung fibrosis and persist in the lung over the life span","volume":"214","author":"Misharin","year":"2017","journal-title":"J. Exp. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1002\/art.38288","article-title":"Association of Interferon- and Transforming Growth Factor \u03b2-Regulated Genes and Macrophage Activation with Systemic Sclerosis-Related Progressive Lung Fibrosis","volume":"66","author":"Christmann","year":"2014","journal-title":"Arthritis Rheumatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1179\/1351000213Y.0000000046","article-title":"Free radicals and endothelial dysfunction: Potential positive effects of TNF-\u03b1 inhibitors","volume":"18","author":"Murdaca","year":"2013","journal-title":"Redox Report"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1038\/nri3070","article-title":"Monocyte recruitment during infection and inflammation","volume":"11","author":"Shi","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.3389\/fimmu.2019.01068","article-title":"L-selectin: A Major Regulator of Leukocyte Adhesion, Migration and Signaling","volume":"10","author":"Ivetic","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"52","DOI":"10.3389\/fmed.2018.00052","article-title":"CD11b Activity Modulates Pathogenesis of Lupus Nephritis","volume":"5","author":"Khan","year":"2018","journal-title":"Front. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1002\/art.41737","article-title":"Regulation of Monocyte Adhesion and Type I Interferon Signaling by CD52 in Patients with Systemic Sclerosis","volume":"73","author":"Rudnik","year":"2021","journal-title":"Arthritis Rheumatol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1097\/MOH.0000000000000389","article-title":"The regulation and importance of monocyte chemoattractant protein-1","volume":"25","author":"Bianconi","year":"2018","journal-title":"Curr. Opin. Hematol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4869","DOI":"10.1007\/s00018-019-03255-6","article-title":"Dual CCR5\/CCR2 targeting: Opportunities for the cure of complex disorders","volume":"76","author":"Fantuzzi","year":"2019","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.3389\/fimmu.2019.02035","article-title":"Human Monocyte Subsets and Phenotypes in Major Chronic Inflammatory Diseases","volume":"10","author":"Kapellos","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_20","first-page":"S44","article-title":"CCL2, CCL3 and CCL5 chemokines in systemic sclerosis: The correlation with SSc clinical features and the effect of prostaglandin E1 treatment","volume":"30","author":"Bandinelli","year":"2012","journal-title":"Clin Exp Rheumatol"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"eaay5947","DOI":"10.1126\/science.aay5947","article-title":"An atlas of the protein-coding genes in the human, pig, and mouse brain","volume":"367","author":"Zhong","year":"2020","journal-title":"Science"},{"key":"ref_22","unstructured":"(2022, January 04). Human Protein Atlas. Available online: https:\/\/www.proteinatlas.org\/ENSG00000188404-SELL\/immune+cell."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"R74","DOI":"10.1186\/ar4251","article-title":"Versican is upregulated in circulating monocytes in patients with systemic sclerosis and amplifies a CCL2-mediated pathogenic loop","volume":"15","author":"Masuda","year":"2013","journal-title":"Arthritis Res. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"758","DOI":"10.3389\/fmed.2020.587773","article-title":"Classical Disease-Specific Autoantibodies in Systemic Sclerosis: Clinical Features, Gene Susceptibility, and Disease Stratification","volume":"7","author":"Yang","year":"2020","journal-title":"Front. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.2169\/internalmedicine.5665-20","article-title":"Anti-PM\/Scl Antibody-positive Systemic Sclerosis Complicated by Multiple Organ Involvement","volume":"60","author":"Shimizu","year":"2021","journal-title":"Intern. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/S0923-1811(01)00128-1","article-title":"Serum levels of interleukin-6 and interleukin-10 correlate with total skin thickness score in patients with systemic sclerosis","volume":"27","author":"Sato","year":"2001","journal-title":"J. Dermatol. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/S0923-1811(00)00108-0","article-title":"Elevated serum levels of soluble L-selectin in patients with systemic sclerosis declined after intravenous injection of lipo-prostaglandin E1","volume":"25","author":"Inaoki","year":"2001","journal-title":"J. Dermatol. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1046\/j.1365-2249.2001.01514.x","article-title":"Elevated serum L-selectin levels and decreased L-selectin expression on CD8+lymphocytes in systemic sclerosis","volume":"124","author":"Shimada","year":"2001","journal-title":"Clin. Exp. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Hasegawa, M., Asano, Y., Endo, H., Fujimoto, M., Goto, D., Ihn, H., Inoue, K., Ishikawa, O., Kawaguchi, Y., and Kuwana, M. (2014). Serum Adhesion Molecule Levels as Prognostic Markers in Patients with Early Systemic Sclerosis: A Multicentre, Prospective, Observational Study. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0088150"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1046\/j.1365-2141.1996.7562378.x","article-title":"Soluble L-selectin in the connective tissue diseases","volume":"95","author":"Blann","year":"1996","journal-title":"Br. J. Haematol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Dunne, J.V., Van Eeden, S.F., and Keen, K.J. (2012). L-selectin and Skin Damage in Systemic Sclerosis. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0044814"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1007\/s100670050047","article-title":"Circulating P- and L-Selectin and T-Lymphocyte Activation in Patients with Autoimmune Rheumatic Diseases","volume":"18","author":"Sfikakis","year":"1999","journal-title":"Clin. Rheumatol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.0300-9475.2004.01389.x","article-title":"Serum-Soluble Selectin Levels in Patients with Rheumatoid Arthritis and Systemic Sclerosis","volume":"59","author":"Ates","year":"2004","journal-title":"Scand. J. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1136\/ard.2004.030338","article-title":"Up regulated expression of tumour necrosis factor {alpha} converting enzyme in peripheral monocytes of patients with early systemic sclerosis","volume":"64","author":"Bohgaki","year":"2005","journal-title":"Ann. Rheum. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.clim.2017.05.013","article-title":"Increased proportions of functionally impaired regulatory T cell subsets in systemic sclerosis","volume":"184","author":"Ugor","year":"2017","journal-title":"Clin. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Radstake, T.R.D.J., Van Bon, L., Broen, J., Wenink, M., Santegoets, K., Deng, Y., Hussaini, A., Simms, R., Cruikshank, W.W., and Lafyatis, R. (2009). Increased Frequency and Compromised Function of T Regulatory Cells in Systemic Sclerosis (SSc) Is Related to a Diminished CD69 and TGF\u03b2 Expression. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0005981"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Impellizzieri, D., Egholm, C., Valaperti, A., Distler, O., and Boyman, O. (2021). Patients with systemic sclerosis show phenotypic and functional defects in neutrophils. Allergy.","DOI":"10.1111\/all.15073"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1111\/j.0022-202X.2005.23767.x","article-title":"Abnormal natural killer cell function in systemic sclerosis: Altered cytokine production and defective killing activity","volume":"125","author":"Horikawa","year":"2005","journal-title":"J. Invest. Dermatol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1136\/ard.2007.080424","article-title":"Bosentan regulates the expression of adhesion molecules on circulating T cells and serum soluble adhesion molecules in systemic sclerosis-associated pulmonary arterial hypertension","volume":"67","author":"Iannone","year":"2008","journal-title":"Ann. Rheum. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1007\/s13205-020-02549-y","article-title":"L-Selectin expression is associated with inflammatory microenvironment and favourable prognosis in breast cancer","volume":"11","author":"Kumari","year":"2021","journal-title":"3 Biotech"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1159\/000053590","article-title":"Interferon-gamma increases CD62L expression on human eosinophils","volume":"120","author":"Momose","year":"1999","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_42","first-page":"1643","article-title":"The pathogenic role of autoantibodies to nuclear autoantigens in systemic sclerosis (scleroderma)","volume":"32","author":"Raymond","year":"2005","journal-title":"J. Rheumatol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"E1461","DOI":"10.1073\/pnas.1417100112","article-title":"L-selectin shedding is activated specifically within transmigrating pseudopods of monocytes to regulate cell polarity in vitro","volume":"112","author":"Rzeniewicz","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"jcs215541","DOI":"10.1242\/jcs.215541","article-title":"Sequential binding of ezrin and moesin to L-selectin regulates monocyte protrusive behaviour during transendothelial migration","volume":"131","author":"Tomlins","year":"2018","journal-title":"J. Cell Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2502","DOI":"10.4049\/jimmunol.0901778","article-title":"Cell adhesion molecules regulate fibrotic process via Th1\/Th2\/Th17 cell balance in a bleomycin-induced scleroderma model","volume":"185","author":"Yoshizaki","year":"2010","journal-title":"J. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"698","DOI":"10.4049\/jimmunol.179.1.698","article-title":"Intercellular Adhesion Molecule-1 Deficiency Attenuates the Development of Skin Fibrosis in Tight-Skin Mice","volume":"179","author":"Matsushita","year":"2007","journal-title":"J. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1183\/09031936.05.00082604","article-title":"CCR5 expression and CC chemokine levels in idiopathic pulmonary fibrosis","volume":"25","author":"Capelli","year":"2005","journal-title":"Eur. Respir. J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1002\/art.23406","article-title":"T cell polarization identifies distinct clinical phenotypes in scleroderma lung disease","volume":"58","author":"Boin","year":"2008","journal-title":"Arthritis Rheum."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1164\/ajrccm.165.2.2106084","article-title":"Increased macrophage inflammatory protein-1alpha and macrophage inflammatory protein-1beta levels in bronchoalveolar lavage fluid of patients affected by different stages of pulmonary sarcoidosis","volume":"165","author":"Capelli","year":"2002","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1186\/s12931-017-0569-y","article-title":"COPD monocytes demonstrate impaired migratory ability","volume":"18","author":"Ravi","year":"2017","journal-title":"Respir. Res."},{"key":"ref_51","first-page":"e738","article-title":"Peripheral blood T4 cell surface CCR5 density as a marker of activity in rheumatoid arthritis treated with anti-CD20 monoclonal antibody","volume":"128","author":"Fabre","year":"2009","journal-title":"Immunology"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"65","DOI":"10.2174\/1570163816666191023103118","article-title":"Influence of \u03b2-D-mannuronic Acid, as a New Member of Non-steroidal Anti- Inflammatory Drugs Family, on the Expression Pattern of Chemokines and their Receptors in Rheumatoid Arthritis","volume":"18","author":"Aslani","year":"2021","journal-title":"Curr. Drug Discov. Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"69","DOI":"10.2174\/1872213X13666191114111822","article-title":"The Situation of Chemokine Ligands and Receptors Gene Expression, Following the Oral Administration of Drug Mannuronic Acid in Rheumatoid Arthritis Patients","volume":"14","author":"Aslani","year":"2020","journal-title":"Recent Pat. Inflamm. Allergy Drug Discov."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1136\/annrheumdis-2013-204424","article-title":"2013 classification criteria for systemic sclerosis: An American college of rheumatology\/European league against rheumatism collaborative initiative","volume":"72","author":"Khanna","year":"2013","journal-title":"Ann. Rheum. Dis."},{"key":"ref_55","first-page":"202","article-title":"Scleroderma (systemic sclerosis): Classification, subsets and pathogenesis","volume":"15","author":"LeRoy","year":"1988","journal-title":"J. Rheumatol."},{"key":"ref_56","unstructured":"Van Venrooij, W.J., and Maini, R.N. (1996). Counterimmunoelectrophoresis and Immunodiffusion for the Detection of Antibodies to Soluble Cellular Antigens. Manual of Biological Markers of Disease, Springer."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Corkum, C.P., Ings, D.P., Burgess, C., Karwowska, S., Kroll, W., and Michalak, T.I. (2015). Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT\u2122) and standard density gradient. BMC Immunol., 16.","DOI":"10.1186\/s12865-015-0113-0"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"21735","DOI":"10.1038\/s41598-020-78750-z","article-title":"Immunoregulation induced by autologous serum collected after acute exercise in obese men: A randomized cross-over trial","volume":"10","author":"Dorneles","year":"2020","journal-title":"Sci. Rep."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/4\/2233\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:21:50Z","timestamp":1760134910000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/4\/2233"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,17]]},"references-count":58,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["ijms23042233"],"URL":"https:\/\/doi.org\/10.3390\/ijms23042233","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,17]]}}}