{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:52:41Z","timestamp":1760151161555,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,19]],"date-time":"2022-02-19T00:00:00Z","timestamp":1645228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Marie Sk\u0142odowska-Curie Innovative Training Network H2020 MSCA-ITN-ETN","award":["No. 721281"],"award-info":[{"award-number":["No. 721281"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Loss of active synapses and alterations in membrane lipids are crucial events in physiological aging as well as in neurodegenerative disorders. Both are related to the abnormal aggregation of amyloid-beta (A\u03b2) species, generally known as amyloidosis. There are two major known human A\u03b2 species: A\u03b2(1\u201340) and A\u03b2(1\u201342). However, which of these species have more influence on active synapses and membrane lipids is still poorly understood. Additionally, the time-dependent effect of A\u03b2 species on alterations in membrane lipids of hippocampal neurones and glial cells remains unknown. Therefore, our study contributes to a better understanding of the role of A\u03b2 species in the loss of active synapses and the dysregulation of membrane lipids in vitro. We showed that A\u03b2(1\u201340) or A\u03b2(1\u201342) treatment influences membrane lipids before synaptic loss appears and that the loss of active synapses is not dependent on the A\u03b2 species. Our lipidomic data analysis showed early changes in specific lipid classes such as sphingolipid and glycerophospholipid neurones. Our results underscore the potential role of lipids as a possible early diagnostic biomarker in amyloidosis-related disorders.<\/jats:p>","DOI":"10.3390\/ijms23042300","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T09:19:39Z","timestamp":1645435179000},"page":"2300","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A\u03b2-Induced Alterations in Membrane Lipids Occur before Synaptic Loss Appears"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0253-4914","authenticated-orcid":false,"given":"Michiel","family":"Van Bulck","sequence":"first","affiliation":[{"name":"Research Group Anatomy, School for Medicine and Health Science, Carl von Ossietzky University Oldenburg, 26129 Oldenburg, Germany"},{"name":"Department of Experimental Models of Human Disease, Networked Center of Biomedical Research on Neurodegenerative Diseases (CIBERNED), Institute for Biomedical Research A. Sols (CSIC-UAM), 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Brandt","sequence":"additional","affiliation":[{"name":"Research Group Anatomy, School for Medicine and Health Science, Carl von Ossietzky University Oldenburg, 26129 Oldenburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7232-4088","authenticated-orcid":false,"given":"Ralf A.","family":"Claus","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology and Intensive Care Medicine Center for Molecular Biomedicine(CMB), Jena University Hospital, 07745 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6650-7849","authenticated-orcid":false,"given":"Markus","family":"Gr\u00e4ler","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology and Intensive Care Medicine Center for Molecular Biomedicine(CMB), Jena University Hospital, 07745 Jena, Germany"},{"name":"Centre for Sepsis Control and Care (CSCC), Jena University Hospital, 07745 Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3651-1470","authenticated-orcid":false,"given":"Anja U.","family":"Br\u00e4uer","sequence":"additional","affiliation":[{"name":"Research Group Anatomy, School for Medicine and Health Science, Carl von Ossietzky University Oldenburg, 26129 Oldenburg, Germany"},{"name":"Research Centre for Neurosensory Science, Carl von Ossietzky University Oldenburg, 26129 Oldenburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"118","DOI":"10.3389\/fnagi.2018.00118","article-title":"The Physiological Roles of Amyloid-\u03b2 Peptide Hint at New Ways to Treat Alzheimer\u2019s Disease","volume":"10","author":"Brothers","year":"2018","journal-title":"Front. 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