{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:39:16Z","timestamp":1790422756173,"version":"4.1.0"},"reference-count":28,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,19]],"date-time":"2022-02-19T00:00:00Z","timestamp":1645228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Arterial calcification is a common feature of pseudoxanthoma elasticum (PXE), a disease characterized by ABCC6 mutations, inducing a deficiency in pyrophosphate, a key inhibitor of calcium phosphate crystallization in arteries. Methods: we analyzed whether long-term exposure of Abcc6\u2212\/\u2212 mice (a murine model of PXE) to a mild vitamin D supplementation, with or without calcium, would impact the development of vascular calcification. Eight groups of mice (including Abcc6\u2212\/\u2212 and wild-type) received vitamin D supplementation every 2 weeks, a calcium-enriched diet alone (calcium in drinking water), both vitamin D supplementation and calcium-enriched diet, or a standard diet (controls) for 6 months. Aorta and kidney artery calcification was assessed by 3D-micro-computed tomography, Optical PhotoThermal IR (OPTIR) spectroscopy, scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDS) and Yasue staining. Results: at 6 months, although vitamin D and\/or calcium did not significantly increase serum calcium levels, vitamin D and calcium supplementation significantly worsened aorta and renal artery calcification in Abcc6\u2212\/\u2212 mice. Conclusions: vitamin D and\/or calcium supplementation accelerate vascular calcification in a murine model of PXE. These results sound a warning regarding the use of these supplementations in PXE patients and, to a larger extent, patients with low systemic pyrophosphate levels.<\/jats:p>","DOI":"10.3390\/ijms23042302","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T09:18:42Z","timestamp":1645435122000},"page":"2302","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Vitamin D and Calcium Supplementation Accelerate Vascular Calcification in a Model of Pseudoxanthoma Elasticum"],"prefix":"10.3390","volume":"23","author":[{"given":"Elise","family":"Bouderlique","sequence":"first","affiliation":[{"name":"UMR S 1155, Sorbonne Universit\u00e9, 75020 Paris, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Sorbonne Universit\u00e9, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellie","family":"Tang","sequence":"additional","affiliation":[{"name":"UMR S 1155, Sorbonne Universit\u00e9, 75020 Paris, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Sorbonne Universit\u00e9, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1175-0440","authenticated-orcid":false,"given":"Jeremy","family":"Zaworski","sequence":"additional","affiliation":[{"name":"UMR S 1155, Sorbonne Universit\u00e9, 75020 Paris, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Sorbonne Universit\u00e9, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"Coudert","sequence":"additional","affiliation":[{"name":"UFR d\u2019odontologie (D\u00e9partement des Sciences Biologiques), Universit\u00e9 Paris Diderot BIOSCAR\u2014INSERM U1132, H\u00f4pital Lariboisi\u00e8re, 75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5112-9061","authenticated-orcid":false,"given":"Dominique","family":"Bazin","sequence":"additional","affiliation":[{"name":"Laboratoire de Physique des Solides, CNRS UMR 8502, Universit\u00e9 Paris Sud XI, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9975-4301","authenticated-orcid":false,"given":"Ferenc","family":"Borondics","sequence":"additional","affiliation":[{"name":"Synchrotron Soleil, L\u2019Orme des Merisiers, Saint-Aubin\u2013BP48, CEDEX, 91192 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2756-2287","authenticated-orcid":false,"given":"Jean-Philippe","family":"Haymann","sequence":"additional","affiliation":[{"name":"UMR S 1155, Sorbonne Universit\u00e9, 75020 Paris, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Sorbonne Universit\u00e9, 75020 Paris, France"},{"name":"Physiology Unit, AP-HP, H\u00f4pital Tenon, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges","family":"Leftheriotis","sequence":"additional","affiliation":[{"name":"Laboratory of Physiology and Molecular Medicine (LP2M), CNRS-UNS UMR 7370, University of Nice, 28 rue de Valombrose, CEDEX 2, 06107 Nice, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovic","family":"Martin","sequence":"additional","affiliation":[{"name":"MITOVASC Institute\u2014UMR CNRS 6015 INSERM U1083 Angers University, 49100 Angers, France"},{"name":"PXE Consultation Center, MAGEC Reference Center, MAGEC Nord Center for Rare Skin Diseases, Angers University Hospital, 49100 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Daudon","sequence":"additional","affiliation":[{"name":"UMR S 1155, Sorbonne Universit\u00e9, 75020 Paris, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Sorbonne Universit\u00e9, 75020 Paris, France"},{"name":"Physiology Unit, AP-HP, H\u00f4pital Tenon, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Letavernier","sequence":"additional","affiliation":[{"name":"UMR S 1155, Sorbonne Universit\u00e9, 75020 Paris, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Sorbonne Universit\u00e9, 75020 Paris, France"},{"name":"Physiology Unit, AP-HP, H\u00f4pital Tenon, 75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1161\/ATVBAHA.116.306717","article-title":"Medial Arterial Calcification","volume":"36","author":"Ho","year":"2016","journal-title":"Arterioscler. 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