{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:55:11Z","timestamp":1760151311402,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T00:00:00Z","timestamp":1645401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001778","name":"Deakin University","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/501100001778","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The IL-2 family of cytokines act via receptor complexes that share the interleukin-2 receptor gamma common (IL-2R\u03b3c) chain to play key roles in lymphopoiesis. Inactivating IL-2R\u03b3c mutations results in severe combined immunodeficiency (SCID) in humans and other species. This study sought to generate an equivalent zebrafish SCID model. The zebrafish il2rga gene was targeted for genome editing using TALENs and presumed loss-of-function alleles analyzed with respect to immune cell development and impacts on intestinal microbiota and tumor immunity. Knockout of zebrafish Il-2r\u03b3c.a resulted in a SCID phenotype, including a significant reduction in T cells, with NK cells also impacted. This resulted in dysregulated intestinal microbiota and defective immunity to tumor xenotransplants. Collectively, this establishes a useful zebrafish SCID model.<\/jats:p>","DOI":"10.3390\/ijms23042385","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:47:45Z","timestamp":1645476465000},"page":"2385","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Generation and Characterization of a Zebrafish IL-2R\u03b3c SCID Model"],"prefix":"10.3390","volume":"23","author":[{"given":"Robert","family":"Sertori","sequence":"first","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3216, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Realla","family":"Jones","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3216, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4850-2205","authenticated-orcid":false,"given":"Faiza","family":"Basheer","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3216, Australia"},{"name":"Institute for Mental and Physical Health and Clinical Translation, Deakin University, Geelong, VIC 3216, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leni","family":"Rivera","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3216, Australia"},{"name":"Institute for Mental and Physical Health and Clinical Translation, Deakin University, Geelong, VIC 3216, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha","family":"Dawson","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3216, Australia"},{"name":"Institute for Mental and Physical Health and Clinical Translation, Deakin University, Geelong, VIC 3216, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5767-6146","authenticated-orcid":false,"given":"Stella","family":"Loke","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Deakin University, Burwood, VIC 3125, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Somayyeh","family":"Heidary","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3216, Australia"},{"name":"Institute for Mental and Physical Health and Clinical Translation, Deakin University, Geelong, VIC 3216, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amardeep","family":"Dhillon","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3216, Australia"},{"name":"Institute for Mental and Physical Health and Clinical Translation, Deakin University, Geelong, VIC 3216, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1646-4905","authenticated-orcid":false,"given":"Clifford","family":"Liongue","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3216, Australia"},{"name":"Institute for Mental and Physical Health and Clinical Translation, Deakin University, Geelong, VIC 3216, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7945-7975","authenticated-orcid":false,"given":"Alister C.","family":"Ward","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3216, Australia"},{"name":"Institute for Mental and Physical Health and Clinical Translation, Deakin University, Geelong, VIC 3216, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.imlet.2006.11.006","article-title":"Common gamma chain cytokines: Dissidence in the details","volume":"108","author":"Alves","year":"2007","journal-title":"Immunol. 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