{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T03:23:13Z","timestamp":1773804193667,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["19-15609S"],"award-info":[{"award-number":["19-15609S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Young sex chromosomes possess unique and ongoing dynamics that allow us to understand processes that have an impact on their evolution and divergence. The genus Silene includes species with evolutionarily young sex chromosomes, and two species of section Melandrium, namely Silene latifolia (24, XY) and Silene dioica (24, XY), are well-established models of sex chromosome evolution, Y chromosome degeneration, and sex determination. In both species, the X and Y chromosomes are strongly heteromorphic and differ in the genomic composition compared to the autosomes. It is generally accepted that for proper cell division, the longest chromosomal arm must not exceed half of the average length of the spindle axis at telophase. Yet, it is not clear what are the dynamics between males and females during mitosis and how the cell compensates for the presence of the large Y chromosome in one sex. Using hydroxyurea cell synchronization and 2D\/3D microscopy, we determined the position of the sex chromosomes during the mitotic cell cycle and determined the upper limit for the expansion of sex chromosome non-recombining region. Using 3D specimen preparations, we found that the velocity of the large chromosomes is compensated by the distant positioning from the central interpolar axis, confirming previous mathematical modulations.<\/jats:p>","DOI":"10.3390\/ijms23052422","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T22:34:30Z","timestamp":1645569270000},"page":"2422","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Sister Chromatid Division of the Heteromorphic Sex Chromosomes in Silene Species and Their Transmissibility towards the Mitosis"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7621-8936","authenticated-orcid":false,"given":"V\u00e1clav","family":"Ba\u010dovsk\u00fd","sequence":"first","affiliation":[{"name":"Department of Plant Developmental Genetics, Institute of Biophysics of the Czech Academy of Sciences, Kralovopolska 135, 612 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1\u0161","family":"Jan\u00ed\u010dek","sequence":"additional","affiliation":[{"name":"Department of Plant Developmental Genetics, Institute of Biophysics of the Czech Academy of Sciences, Kralovopolska 135, 612 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"Hobza","sequence":"additional","affiliation":[{"name":"Department of Plant Developmental Genetics, Institute of Biophysics of the Czech Academy of Sciences, Kralovopolska 135, 612 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1038\/nrg3366","article-title":"Y-chromosome evolution: Emerging insights into processes of Y-chromosome degeneration","volume":"14","author":"Bachtrog","year":"2013","journal-title":"Nat. Rev. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1111\/nph.16002","article-title":"Young sex chromosomes in plants and animals","volume":"224","author":"Charlesworth","year":"2019","journal-title":"New Phytol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/j.cell.2006.02.024","article-title":"Sex chromosome specialization and degeneration in mammals","volume":"124","author":"Graves","year":"2006","journal-title":"Cell"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Bachtrog, D., Mank, J.E., Peichel, C.L., Kirkpatrick, M., Otto, S.P., Ashman, T.-L., Hahn, M.W., Kitano, J., Mayrose, I., and Ming, R. (2014). Sex determination: Why so many ways of doing it?. PLoS Biol., 12.","DOI":"10.1371\/journal.pbio.1001899"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.tig.2018.04.001","article-title":"Why do sex chromosomes stop recombining?","volume":"34","author":"Ponnikas","year":"2018","journal-title":"Trends Genet."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1534\/genetics.105.044040","article-title":"Blocked recombination along the mating-type chromosomes of Neurospora tetrasperma involves both structural heterozygosity and autosomal genes","volume":"171","author":"Jacobson","year":"2005","journal-title":"Genetics"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Na, J.-K., Wang, J., Murray, J.E., Gschwend, A.R., Zhang, W., Yu, Q., P\u00e9rez, R., Feltus, F., Chen, C., and Kubat, Z. (2012). Construction of physical maps for the sex-specific regions of papaya sex chromosomes. BMC Genom., 13.","DOI":"10.1186\/1471-2164-13-176"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1534\/genetics.114.173021","article-title":"Evidence for emergence of sex-determining gene(s) in a centromeric region in Vasconcellea parviflora","volume":"199","author":"Iovene","year":"2015","journal-title":"Genetics"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.tig.2011.05.005","article-title":"Are all sex chromosomes created equal?","volume":"27","author":"Bachtrog","year":"2011","journal-title":"Trends Genet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1146\/annurev-arplant-043015-111911","article-title":"Plant sex chromosomes","volume":"67","author":"Charlesworth","year":"2016","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6422","DOI":"10.1038\/s41598-017-06822-8","article-title":"High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis","volume":"7","author":"Dias","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1093\/aob\/mcaa160","article-title":"Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa","volume":"127","author":"Jesionek","year":"2021","journal-title":"Ann. Bot."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1007\/s10577-015-9496-2","article-title":"Impact of repetitive DNA on sex chromosome evolution in plants","volume":"23","author":"Hobza","year":"2015","journal-title":"Chromosom. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s10577-017-9555-y","article-title":"Cytogenetic comparison of heteromorphic and homomorphic sex chromosomes in Coccinia (Cucurbitaceae) points to sex chromosome turnover","volume":"25","author":"Sousa","year":"2017","journal-title":"Chromosom. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"205","DOI":"10.3389\/fpls.2020.00205","article-title":"The formation of sex chromosomes in Silene latifolia and S. dioica was accompanied by multiple chromosomal rearrangements","volume":"11","author":"Hobza","year":"2020","journal-title":"Front. Plant Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"12087","DOI":"10.1038\/ncomms12087","article-title":"How to make a sex chromosome","volume":"7","author":"Wright","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1093\/oxfordjournals.molbev.a004147","article-title":"Substitution rates in the X- and Y-linked genes of the plants, Silene latifolia and S. dioica","volume":"19","author":"Filatov","year":"2002","journal-title":"Mol. Biol. Evol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1016\/j.cub.2018.04.069","article-title":"The mutation rate and the age of the sex chromosomes in Silene latifolia","volume":"28","author":"Krasovec","year":"2018","journal-title":"Curr. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1139\/G08-024","article-title":"Microsatellite accumulation on the Y chromosome in Silene latifolia","volume":"51","author":"Kubat","year":"2008","journal-title":"Genome"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Puterova, J., Kubat, Z., Kejnovsky, E., Jesionek, W., Cizkova, J., Vyskot, B., and Hobza, R. (2018). The slowdown of Y chromosome expansion in dioecious Silene latifolia due to DNA loss and male-specific silencing of retrotransposons. BMC Genom., 19.","DOI":"10.1186\/s12864-018-4547-7"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1534\/genetics.108.099267","article-title":"Recent spread of a retrotransposon in the Silene latifolia genome, apart from the Y chromosome","volume":"181","author":"Filatov","year":"2009","journal-title":"Genetics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1016\/j.cub.2015.03.015","article-title":"Gene loss from a plant sex chromosome system","volume":"25","author":"Bergero","year":"2015","journal-title":"Curr. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"13021","DOI":"10.1073\/pnas.1508454112","article-title":"Rapid Y degeneration and dosage compensation in plant sex chromosomes","volume":"112","author":"Papadopulos","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1038\/s41477-018-0221-y","article-title":"Genomic imprinting mediates dosage compensation in a young plant XY system","volume":"4","author":"Muyle","year":"2018","journal-title":"Nat. Plants"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1007\/s00425-019-03182-7","article-title":"The distribution of epigenetic histone marks differs between the X and Y chromosomes in Silene latifolia","volume":"250","author":"Houben","year":"2019","journal-title":"Planta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"110528","DOI":"10.1016\/j.plantsci.2020.110528","article-title":"Histone post-translational modifications in Silene latifolia X and Y chromosomes suggest a mammal-like dosage compensation system","volume":"299","author":"Vyskot","year":"2020","journal-title":"Plant Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0092-8674(00)81891-7","article-title":"There is an upper limit of chromosome size for normal development of an organism","volume":"88","author":"Schubert","year":"1997","journal-title":"Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1159\/000068530","article-title":"Barley chromosome arms longer than half of the spindle axis interfere with nuclear divisions","volume":"98","author":"Hudakova","year":"2002","journal-title":"Cytogenet. Genome Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1159\/000056981","article-title":"Alteration of chromosome numbers by generation of minichromosomes\u2013Is there a lower limit of chromosome size for stable segregation?","volume":"93","author":"Schubert","year":"2001","journal-title":"Cytogenet. Genome Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.pbi.2007.01.001","article-title":"Chromosome evolution","volume":"10","author":"Schubert","year":"2007","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1093\/molbev\/msr011","article-title":"Chromosome size in diploid eukaryotic species centers on the average length with a conserved boundary","volume":"28","author":"Li","year":"2011","journal-title":"Mol. Biol. Evol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Kuznetsova, M.A., Chaban, I.A., and Sheval, E. (2017). V Visualization of chromosome condensation in plants with large chromosomes. BMC Plant Biol., 17.","DOI":"10.1186\/s12870-017-1102-7"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.15252\/embj.201798014","article-title":"Chromosome structure dynamics during the cell cycle: A structure to fit every phase","volume":"36","author":"Barrington","year":"2017","journal-title":"EMBO J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"5349","DOI":"10.1073\/pnas.0601215103","article-title":"The influence of chromosome flexibility on chromosome transport during anaphase A","volume":"103","author":"Raj","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1083\/jcb.25.1.119","article-title":"Chromosome velocity during mitosis as a function of chromosome size and position","volume":"25","author":"Nicklas","year":"1965","journal-title":"J. Cell Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/BF01283395","article-title":"Sex chromatin and nucleolar analyses in Rumex acetosa L.","volume":"217","author":"Lengerova","year":"2001","journal-title":"Protoplasma"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1007\/s10577-008-1254-2","article-title":"Survey of repetitive sequences in Silene latifolia with respect to their distribution on sex chromosomes","volume":"16","author":"Cermak","year":"2008","journal-title":"Chromosom. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.bbaexp.2003.11.017","article-title":"Homologous pairing and chromosome dynamics in meiosis and mitosis","volume":"1677","author":"McKee","year":"2004","journal-title":"Biochim. Biophys. Acta-Gene Struct. Expr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.devcel.2014.04.009","article-title":"Exploring the function of cell shape and size during mitosis","volume":"29","author":"Cadart","year":"2014","journal-title":"Dev. Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1083\/jcb.201502092","article-title":"Mitotic chromosome length scales in response to both cell and nuclear size","volume":"209","author":"Ladouceur","year":"2015","journal-title":"J. Cell Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.semcdb.2014.07.008","article-title":"Force and the spindle: Mechanical cues in mitotic spindle orientation","volume":"34","author":"Goddard","year":"2014","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/s00709-015-0797-2","article-title":"Chromosomal localization of 45S rDNA, sex-specific C values, and heterochromatin distribution in Coccinia grandis (L.) Voigt","volume":"253","author":"Bhowmick","year":"2016","journal-title":"Protoplasma"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Mand\u00e1kov\u00e1, T., and Lysak, M.A. (2016). Chromosome preparation for cytogenetic analyses in Arabidopsis. Current Protocols in Plant Biology, John Wiley & Sons, Inc.","DOI":"10.1002\/cppb.20009"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1007\/s00412-018-0660-2","article-title":"A guide to classifying mitotic stages and mitotic defects in fixed cells","volume":"127","author":"Baudoin","year":"2018","journal-title":"Chromosoma"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1759-8753-2-4","article-title":"Plant centromeric retrotransposons: A structural and cytogenetic perspective","volume":"2","author":"Neumann","year":"2011","journal-title":"Mob. DNA"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/tpj.13942","article-title":"A cytological approach to studying meiotic recombination and chromosome dynamics in Arabidopsis thaliana male meiocytes in three dimensions","volume":"95","author":"Hurel","year":"2018","journal-title":"Plant J."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/5\/2422\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:25:06Z","timestamp":1760135106000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/5\/2422"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,22]]},"references-count":46,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,3]]}},"alternative-id":["ijms23052422"],"URL":"https:\/\/doi.org\/10.3390\/ijms23052422","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,22]]}}}