{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:46:10Z","timestamp":1790880370545,"version":"4.1.0"},"reference-count":28,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:00:00Z","timestamp":1645574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2017\/16482-5"],"award-info":[{"award-number":["2017\/16482-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["202186\/2018-0 ESN"],"award-info":[{"award-number":["202186\/2018-0 ESN"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Food-derived bioactive peptides are of great interest to science and industry due to evolving drivers of food product innovation, including health and wellness. This study aims to draw attention through a critical study on how bioinformatics analysis is employed in the identification of bioactive peptides in the laboratory. An in silico analysis (PeptideRanker, BIOPEP, AHTpin, and mAHTPred) of a list of peptides from goat casein hydrolysate was performed to predict which sequences could potentially be bioactive. To validate the predictions, the in vitro antihypertensive potential of the five peptides with the highest potential was first measured. Then, for three of these, gastrointestinal digestion was simulated in vitro, followed by the analysis of the resulting ACE inhibitory activity as well as antioxidant capacity. We thus observed that the use of new computational biology technologies to predict peptide sequences is an important research tool, but they should not be used alone and complementarity with various in vitro and in vivo assays is essential.<\/jats:p>","DOI":"10.3390\/ijms23052439","type":"journal-article","created":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T09:34:38Z","timestamp":1645608878000},"page":"2439","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Screening of Novel Bioactive Peptides from Goat Casein: In Silico to In Vitro Validation"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4496-2001","authenticated-orcid":false,"given":"Ezequiel R.","family":"Coscueta","sequence":"first","affiliation":[{"name":"Centro de Biotecnologia e Qu\u00edmica Fina (CBQF)-Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3007-7263","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Batista","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia e Qu\u00edmica Fina (CBQF)-Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"},{"name":"Human Neurobehavioral Laboratory\/Research Centre for Human Development (HNL\/CEDH), Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5444-8714","authenticated-orcid":false,"given":"Jos\u00e9 Erick Galindo","family":"Gomes","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias, Letras e Ci\u00eancias Exatas (IBILCE)-Rua Cristov\u00e3o Colombo, Campus S\u00e3o Jos\u00e9 do Rio Preto, Universidade Estadual Paulista (UNESP), 2265, Jardim Nazareth, S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"},{"name":"Laborat\u00f3rio de Microbiologia, Tecnologia Enzim\u00e1tica e Bioprodutos\u2014Universidade Federal do Agreste de Pernambuco (UFAPE), Avenida Bom Pastor s\/n, Boa Vista, Garanhuns 55292-270, PE, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1468-5752","authenticated-orcid":false,"given":"Roberto","family":"da Silva","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias, Letras e Ci\u00eancias Exatas (IBILCE)-Rua Cristov\u00e3o Colombo, Campus S\u00e3o Jos\u00e9 do Rio Preto, Universidade Estadual Paulista (UNESP), 2265, Jardim Nazareth, S\u00e3o Jos\u00e9 do Rio Preto 15054-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Manuela","family":"Pintado","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia e Qu\u00edmica Fina (CBQF)-Laborat\u00f3rio Associado, Escola Superior de Biotecnologia, Universidade Cat\u00f3lica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,23]]},"reference":[{"key":"ref_1","unstructured":"Singh, B.P., Aluko, R.E., Hati, S., and Solanki, D. 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