{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T20:00:58Z","timestamp":1772049658735,"version":"3.50.1"},"reference-count":67,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T00:00:00Z","timestamp":1646006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"National Science Center","doi-asserted-by":"publisher","award":["2017\/27\/B\/NZ7\/01976"],"award-info":[{"award-number":["2017\/27\/B\/NZ7\/01976"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Skeletal muscles are an important reservoir of nitric oxide (NO\u2022) stored in the form of nitrite [NO2\u2212] and nitrate [NO3\u2212] (NOx). Nitrite, which can be reduced to NO\u2022 under hypoxic and acidotic conditions, is considered a physiologically relevant, direct source of bioactive NO\u2022. The aim of the present study was to determine the basal levels of NOx in striated muscles (including rat heart and locomotory muscles) with varied contents of tissue nitrite reductases, such as myoglobin and mitochondrial electron transport chain proteins (ETC-proteins). Muscle NOx was determined using a high-performance liquid chromatography-based method. Muscle proteins were evaluated using western-immunoblotting. We found that oxidative muscles with a higher content of ETC-proteins and myoglobin (such as the heart and slow-twitch locomotory muscles) have lower [NO2\u2212] compared to fast-twitch muscles with a lower content of those proteins. The muscle type had no observed effect on the [NO3\u2212]. Our results demonstrated that fast-twitch muscles possess greater potential to generate NO\u2022 via nitrite reduction than slow-twitch muscles and the heart. This property might be of special importance for fast skeletal muscles during strenuous exercise and\/or hypoxia since it might support muscle blood flow via additional NO\u2022 provision (acidic\/hypoxic vasodilation) and delay muscle fatigue.<\/jats:p>","DOI":"10.3390\/ijms23052686","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T20:11:14Z","timestamp":1646079074000},"page":"2686","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Nitrite Concentration in the Striated Muscles Is Reversely Related to Myoglobin and Mitochondrial Proteins Content in Rats"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0898-1707","authenticated-orcid":false,"given":"Joanna","family":"Majerczak","sequence":"first","affiliation":[{"name":"Chair of Exercise Physiology and Muscle Bioenergetics, Faculty of Health Sciences, Jagiellonian University Medical College, 31-066 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Kij","sequence":"additional","affiliation":[{"name":"Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, 30-348 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna","family":"Drzymala-Celichowska","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Faculty of Health Sciences, Poznan University of Physical Education, 61-871 Poznan, Poland"},{"name":"Department of Physiology and Biochemistry, Faculty of Health Sciences, Poznan University of Physical Education, 61-871 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamil","family":"Kus","sequence":"additional","affiliation":[{"name":"Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, 30-348 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8869-3216","authenticated-orcid":false,"given":"Janusz","family":"Karasinski","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Jagiellonian University, 30-387 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2746-2534","authenticated-orcid":false,"given":"Zenon","family":"Nieckarz","sequence":"additional","affiliation":[{"name":"Experimental Computer Physics Department, Marian Smoluchowski Institute of Physics, Jagiellonian University, 30-348 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6438-8540","authenticated-orcid":false,"given":"Marcin","family":"Grandys","sequence":"additional","affiliation":[{"name":"Chair of Exercise Physiology and Muscle Bioenergetics, Faculty of Health Sciences, Jagiellonian University Medical College, 31-066 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Celichowski","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Faculty of Health Sciences, Poznan University of Physical Education, 61-871 Poznan, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zbigniew","family":"Szkutnik","sequence":"additional","affiliation":[{"name":"Faculty of Applied Mathematics, AGH-University of Science and Technology, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike B.","family":"Hendgen-Cotta","sequence":"additional","affiliation":[{"name":"Department of Cardiology and Vascular Medicine, West German Heart and Vascular Center, University Hospital Essen, Medical Faculty, University of Duisburg-Essen, 45147 Essen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerzy A.","family":"Zoladz","sequence":"additional","affiliation":[{"name":"Chair of Exercise Physiology and Muscle Bioenergetics, Faculty of Health Sciences, Jagiellonian University Medical College, 31-066 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/j.1365-2362.1991.tb01383.x","article-title":"Endogenous nitric oxide: Physiology, pathology and clinical relevance","volume":"21","author":"Moncada","year":"1991","journal-title":"Eur. 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