{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T02:07:33Z","timestamp":1776737253146,"version":"3.51.2"},"reference-count":31,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T00:00:00Z","timestamp":1646352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health","award":["Ricerca Finalizzata RF-2013-02355543"],"award-info":[{"award-number":["Ricerca Finalizzata RF-2013-02355543"]}]},{"name":"Italian Ministry of Health","award":["Ricerca Corrente ID 2757640MPP 2B"],"award-info":[{"award-number":["Ricerca Corrente ID 2757640MPP 2B"]}]},{"name":"Italian Ministry of Health, Italy","award":["Ricerca Corrente ID 2755301 MPP 1A"],"award-info":[{"award-number":["Ricerca Corrente ID 2755301 MPP 1A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Prenylcysteine Oxidase 1 (PCYOX1) is an enzyme involved in the degradation of prenylated proteins. It is expressed in different tissues including vascular and blood cells. We recently showed that the secretome from Pcyox1-silenced cells reduced platelet adhesion both to fibrinogen and endothelial cells, suggesting a potential contribution of PCYOX1 into thrombus formation. Here, we show that in vivo thrombus formation after FeCl3 injury of the carotid artery was delayed in Pcyox1\u2212\/\u2212 mice, which were also protected from collagen\/epinephrine induced thromboembolism. The Pcyox1\u2212\/\u2212 mice displayed normal blood cells count, vascular procoagulant activity and plasma fibrinogen levels. Deletion of Pcyox1 reduced the platelet\/leukocyte aggregates in whole blood, as well as the platelet aggregation, the alpha granules release, and the \u03b1IIb\u03b23 integrin activation in platelet-rich plasma, in response to adenosine diphosphate (ADP) or thrombin receptor agonist peptide (TRAP). Washed platelets from the Pcyox1\u2212\/\u2212 and WT animals showed similar phosphorylation pathway activation, adhesion ability and aggregation. The presence of Pcyox1\u2212\/\u2212 plasma impaired agonist-induced WT platelet aggregation. Our findings show that the absence of PCYOX1 results in platelet hypo-reactivity and impaired arterial thrombosis, and indicates that PCYOX1 could be a novel target for antithrombotic drugs.<\/jats:p>","DOI":"10.3390\/ijms23052831","type":"journal-article","created":{"date-parts":[[2022,3,6]],"date-time":"2022-03-06T20:38:16Z","timestamp":1646599096000},"page":"2831","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Prenylcysteine Oxidase 1 (PCYOX1), a New Player in Thrombosis"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3346-9879","authenticated-orcid":false,"given":"Cristina","family":"Banfi","sequence":"first","affiliation":[{"name":"Unit of Functional Proteomics, Metabolomics and Network Analysis, Centro Cardiologico Monzino IRCCS, 20138 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrizia","family":"Amadio","sequence":"additional","affiliation":[{"name":"Unit of Brain-Heart Axis, Centro Cardiologico Monzino IRCCS, 20138 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7538-4393","authenticated-orcid":false,"given":"Marta","family":"Zar\u00e0","sequence":"additional","affiliation":[{"name":"Unit of Brain-Heart Axis, Centro Cardiologico Monzino IRCCS, 20138 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4706-4418","authenticated-orcid":false,"given":"Maura","family":"Brioschi","sequence":"additional","affiliation":[{"name":"Unit of Functional Proteomics, Metabolomics and Network Analysis, Centro Cardiologico Monzino IRCCS, 20138 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9878-8016","authenticated-orcid":false,"given":"Leonardo","family":"Sandrini","sequence":"additional","affiliation":[{"name":"Unit of Brain-Heart Axis, Centro Cardiologico Monzino IRCCS, 20138 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7486-2637","authenticated-orcid":false,"given":"Silvia S.","family":"Barbieri","sequence":"additional","affiliation":[{"name":"Unit of Brain-Heart Axis, Centro Cardiologico Monzino IRCCS, 20138 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1038\/nm1102-1227","article-title":"Platelets in atherothrombosis","volume":"8","author":"Ruggeri","year":"2002","journal-title":"Nat. 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