{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T14:47:37Z","timestamp":1783435657966,"version":"3.54.6"},"reference-count":62,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:00:00Z","timestamp":1646438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["DPC20171138957"],"award-info":[{"award-number":["DPC20171138957"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["DEQ20160334924"],"award-info":[{"award-number":["DEQ20160334924"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-18-CE14-0016-01"],"award-info":[{"award-number":["ANR-18-CE14-0016-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004431","name":"Fondation de France","doi-asserted-by":"publisher","award":["00086486"],"award-info":[{"award-number":["00086486"]}],"id":[{"id":"10.13039\/501100004431","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Flow-mediated dilation (FMD) of resistance arteries is essential for tissue perfusion but it decreases with ageing. As estrogen receptor alpha (Er\u03b1 encoded by Esr1), and more precisely membrane ER\u03b1, plays an important role in FMD in young mice in a ligand-independent fashion, we evaluated its influence on this arteriolar function in ageing. We first confirmed that in young (6-month-old) mice, FMD of mesenteric resistance arteries was reduced in Esr1\u2212\/\u2212 (lacking ER\u03b1) and C451A-ER\u03b1 (lacking membrane ER\u03b1). In old (24-month-old) mice, FMD was reduced in WT mice compared to young mice, whereas it was not further decreased in Esr1\u2212\/\u2212 and C451A-ER\u03b1 mice. Markers of oxidative stress were similarly increased in old WT and C451A-ER\u03b1 mice. Reduction in oxidative stress with superoxide dismutase plus catalase or Mito-tempo, which reduces mitochondrial superoxide restored FMD to a normal control level in young C451A-ER\u03b1 mice as well as in old WT mice and old C451A-ER\u03b1 mice. Estradiol-mediated dilation was absent in old WT mice. We conclude that oxidative stress is a key event in the decline of FMD, and that an early defect in membrane ER\u03b1 recapitulates phenotypically and functionally ageing of these resistance arteries. The loss of this function could take part in vascular ageing.<\/jats:p>","DOI":"10.3390\/ijms23052862","type":"journal-article","created":{"date-parts":[[2022,3,6]],"date-time":"2022-03-06T20:38:16Z","timestamp":1646599096000},"page":"2862","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Early Inactivation of Membrane Estrogen Receptor Alpha (ER\u03b1) Recapitulates the Endothelial Dysfunction of Aged Mouse Resistance Arteries"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1044-0950","authenticated-orcid":false,"given":"Julie","family":"Favre","sequence":"first","affiliation":[{"name":"MitoVasc Department, Team 2 (CarMe) and CARFI Facility, Angers University, F-49045 Angers, France"},{"name":"Centre National de la Recherche Scientifique (CNRS), UMR 6015, F-49045 Angers, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1083, F-49045 Angers, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), UMR S 1121, Centre de Recherche en Biom\u00e9decine de Strasbourg (CRBS), F-67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Vessieres","sequence":"additional","affiliation":[{"name":"MitoVasc Department, Team 2 (CarMe) and CARFI Facility, Angers University, F-49045 Angers, France"},{"name":"Centre National de la Recherche Scientifique (CNRS), UMR 6015, F-49045 Angers, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1083, F-49045 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Laure","family":"Guihot","sequence":"additional","affiliation":[{"name":"MitoVasc Department, Team 2 (CarMe) and CARFI Facility, Angers University, F-49045 Angers, France"},{"name":"Centre National de la Recherche Scientifique (CNRS), UMR 6015, F-49045 Angers, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1083, F-49045 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Grimaud","sequence":"additional","affiliation":[{"name":"MitoVasc Department, Team 2 (CarMe) and CARFI Facility, Angers University, F-49045 Angers, France"},{"name":"Centre National de la Recherche Scientifique (CNRS), UMR 6015, F-49045 Angers, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1083, F-49045 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coralyne","family":"Proux","sequence":"additional","affiliation":[{"name":"MitoVasc Department, Team 2 (CarMe) and CARFI Facility, Angers University, F-49045 Angers, France"},{"name":"Centre National de la Recherche Scientifique (CNRS), UMR 6015, F-49045 Angers, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1083, F-49045 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3397-2335","authenticated-orcid":false,"given":"Laurent","family":"Loufrani","sequence":"additional","affiliation":[{"name":"MitoVasc Department, Team 2 (CarMe) and CARFI Facility, Angers University, F-49045 Angers, France"},{"name":"Centre National de la Recherche Scientifique (CNRS), UMR 6015, F-49045 Angers, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1083, F-49045 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0024-7241","authenticated-orcid":false,"given":"Fran\u00e7oise","family":"Lenfant","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1297, Institut des Maladies M\u00e9taboliques et Cardiovasculaires (I2MC), Universit\u00e9 Toulouse III Paul Sabatier, CHU Rangueil (University Hospital) de Toulouse, F-31432 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7253-922X","authenticated-orcid":false,"given":"Coralie","family":"Fontaine","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1297, Institut des Maladies M\u00e9taboliques et Cardiovasculaires (I2MC), Universit\u00e9 Toulouse III Paul Sabatier, CHU Rangueil (University Hospital) de Toulouse, F-31432 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2229-6300","authenticated-orcid":false,"given":"Jean-Fran\u00e7ois","family":"Arnal","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1297, Institut des Maladies M\u00e9taboliques et Cardiovasculaires (I2MC), Universit\u00e9 Toulouse III Paul Sabatier, CHU Rangueil (University Hospital) de Toulouse, F-31432 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1094-0285","authenticated-orcid":false,"given":"Daniel","family":"Henrion","sequence":"additional","affiliation":[{"name":"MitoVasc Department, Team 2 (CarMe) and CARFI Facility, Angers University, F-49045 Angers, France"},{"name":"Centre National de la Recherche Scientifique (CNRS), UMR 6015, F-49045 Angers, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), U1083, F-49045 Angers, France"},{"name":"Angers University Hospital (CHU), F-49000 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1007\/s11906-006-0046-3","article-title":"Small artery remodeling in hypertension and 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