{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T17:37:47Z","timestamp":1778002667353,"version":"3.51.4"},"reference-count":67,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,13]],"date-time":"2022-03-13T00:00:00Z","timestamp":1647129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1A2C1004669"],"award-info":[{"award-number":["2021R1A2C1004669"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"GIST Research Institute grant","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]},{"name":"Korea Basic Science Institute under the R&amp;D program","award":["Project No. C140440"],"award-info":[{"award-number":["Project No. C140440"]}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI-2051595"],"award-info":[{"award-number":["DBI-2051595"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI-1902076"],"award-info":[{"award-number":["DBI-1902076"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Colorado-Denver","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Proteins from Sulfolobus solfataricus (S. solfataricus), an extremophile, are active even at high temperatures. The single-stranded DNA (ssDNA) binding protein of S. solfataricus (SsoSSB) is overexpressed to protect ssDNA during DNA metabolism. Although SsoSSB has the potential to be applied in various areas, its structural and ssDNA binding properties at high temperatures have not been studied. We present the solution structure, backbone dynamics, and ssDNA binding properties of SsoSSB at 50 \u00b0C. The overall structure is consistent with the structures previously studied at room temperature. However, the loop between the first two \u03b2 sheets, which is flexible and is expected to undergo conformational change upon ssDNA binding, shows a difference from the ssDNA bound structure. The ssDNA binding ability was maintained at high temperature, but different interactions were observed depending on the temperature. Backbone dynamics at high temperature showed that the rigidity of the structured region was well maintained. The investigation of an N-terminal deletion mutant revealed that it is important for maintaining thermostability, structure, and ssDNA binding ability. The structural and dynamic properties of SsoSSB observed at high temperature can provide information on the behavior of proteins in thermophiles at the molecular level and guide the development of new experimental techniques.<\/jats:p>","DOI":"10.3390\/ijms23063099","type":"journal-article","created":{"date-parts":[[2022,3,13]],"date-time":"2022-03-13T22:29:43Z","timestamp":1647210583000},"page":"3099","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["NMR Structure and Biophysical Characterization of Thermophilic Single-Stranded DNA Binding Protein from Sulfolobus Solfataricus"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7143-6483","authenticated-orcid":false,"given":"Min June","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinwoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9156-6477","authenticated-orcid":false,"given":"Yeongjoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Colorado Denver, Denver, CO 80217-3364, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0964-203X","authenticated-orcid":false,"given":"Woonghee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Colorado Denver, Denver, CO 80217-3364, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chin-Ju","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/BF00408082","article-title":"Sulfolobus: A new genus of sulfur-oxidizing bacteria living at low pH and high temperature","volume":"84","author":"Brock","year":"1972","journal-title":"Arch. 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