{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:55:15Z","timestamp":1760151315944,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,3,13]],"date-time":"2022-03-13T00:00:00Z","timestamp":1647129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002794","name":"Swedish Cancer Society","doi-asserted-by":"publisher","award":["2014"],"award-info":[{"award-number":["2014"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Marcus Borgstr\u00f6ms Foundation","award":["2016"],"award-info":[{"award-number":["2016"]}]},{"name":"L\u00e4ndells Foundation","award":["2016"],"award-info":[{"award-number":["2016"]}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2015"],"award-info":[{"award-number":["2015"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>RNA molecules can adopt specific RNA triplex structures to execute critical biological functions. Human adenoviruses (HAdVs) are abundant pathogens encoding the essential, noncoding virus-associated RNA I (VA RNAI). Here, we employ a triplex-specific probing assay, based on the intercalating and cleaving agent benzoquinoquinoxaline 1, 10-phenanthroline (BQQ\u2013OP), to unravel a potential RNA triplex formation in VA RNAI. The BQQ\u2013OP cleavage of the pathogenic HAdV type 4 (HAdV-4) VA RNAI indicates that a potential triplex is formed involving the highly conserved stem 4 of the central domain and side stem 7. Further, the integrity of the HAdV-4 VA RNAI side stem 7 contributes to a potential triplex formation in vitro and virus growth in vivo. Collectively, we propose that the HAdV-4 VA RNAI can potentially form a biologically relevant triplex structure.<\/jats:p>","DOI":"10.3390\/ijms23063103","type":"journal-article","created":{"date-parts":[[2022,3,13]],"date-time":"2022-03-13T22:29:43Z","timestamp":1647210583000},"page":"3103","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I"],"prefix":"10.3390","volume":"23","author":[{"given":"Helen","family":"Bergquist","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Uppsala University, BMC Box 582, Husargatan 3, SE-751 23 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1344-3962","authenticated-orcid":false,"given":"Raviteja","family":"Inturi","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Uppsala University, BMC Box 582, Husargatan 3, SE-751 23 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rula","family":"Zain","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Translational Research Center Karolinska (TRACK), Karolinska Institutet, Huddinge, SE-141 86 Stockholm, Sweden"},{"name":"Centre for Rare Diseases, Karolinska University Hospital, SE-171 76 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0561-367X","authenticated-orcid":false,"given":"Tanel","family":"Punga","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Uppsala University, BMC Box 582, Husargatan 3, SE-751 23 Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1038\/nrg3722","article-title":"The rise of regulatory RNA","volume":"15","author":"Morris","year":"2014","journal-title":"Nat. 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