{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:52:00Z","timestamp":1787320320387,"version":"build-2736575974"},"reference-count":105,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T00:00:00Z","timestamp":1647907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n Seneca","doi-asserted-by":"publisher","award":["20897\/PI\/18"],"award-info":[{"award-number":["20897\/PI\/18"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PGC2018-097452-B-I00"],"award-info":[{"award-number":["PGC2018-097452-B-I00"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The study of the Mucoralean fungi physiology is a neglected field that the lack of effective genetic tools has hampered in the past. However, the emerging fungal infection caused by these fungi, known as mucormycosis, has prompted many researchers to study the pathogenic potential of Mucorales. The main reasons for this current attraction to study mucormycosis are its high lethality, the lack of effective antifungal drugs, and its recent increased incidence. The most contemporary example of the emergence character of mucormycosis is the epidemics declared in several Asian countries as a direct consequence of the COVID-19 pandemic. Fortunately, this pressure to understand mucormycosis and develop new treatment strategies has encouraged the blossoming of new genetic techniques and methodologies. This review describes the history of genetic manipulation in Mucorales, highlighting the development of methods and how they allowed the main genetic studies in these fungi. Moreover, we have emphasized the recent development of new genetic models to study mucormycosis, a landmark in the field that will configure future research related to this disease.<\/jats:p>","DOI":"10.3390\/ijms23073454","type":"journal-article","created":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T14:55:35Z","timestamp":1647960935000},"page":"3454","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2059-303X","authenticated-orcid":false,"given":"Carlos","family":"Lax","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6608-4145","authenticated-orcid":false,"given":"Jos\u00e9 Tom\u00e1s","family":"C\u00e1novas-M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5406-1613","authenticated-orcid":false,"given":"Ghizlane","family":"Tahiri","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3091-4378","authenticated-orcid":false,"given":"Eusebio","family":"Navarro","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7605-1726","authenticated-orcid":false,"given":"Victoriano","family":"Garre","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3762-5971","authenticated-orcid":false,"given":"Francisco Esteban","family":"Nicol\u00e1s","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica y Microbiolog\u00eda, Facultad de Biolog\u00eda, Universidad de Murcia, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/j.1574-6976.2001.tb00588.x","article-title":"Phycomyces and the biology of light and color","volume":"25","year":"2001","journal-title":"FEMS Microbiol. 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