{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T12:43:23Z","timestamp":1789044203782,"version":"build-2803163510"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:00:00Z","timestamp":1648598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["20-15-00353"],"award-info":[{"award-number":["20-15-00353"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hypertrophic cardiomyopathy (HCM) is the most common inherited myocardial disease with significant genetic and phenotypic heterogeneity. To search for novel biomarkers, which could increase the accuracy of HCM diagnosis and improve understanding of its phenotype formation, we analyzed the levels of circulating miRNAs\u2014stable non-coding RNAs involved in post-transcriptional gene regulation. Performed high throughput sequencing of miRNAs in plasma of HCM patients and controls pinpointed miR-499a-5p as one of 35 miRNAs dysregulated in HCM. Further investigation on enlarged groups of individuals showed that its level was higher in carriers of pathogenic\/likely pathogenic (P\/LP) variants in MYH7 gene compared to controls (fold change, FC = 8.9; p &lt; 0.0001). Just as important, carriers of variants in MYH7 gene were defined with higher miRNA levels than carriers of variants in the MYBPC3 gene (FC = 14.1; p = 0.0003) and other patients (FC = 4.1; p = 0.0008). The receiver operating characteristic analysis analysis showed the ability of miR-499a-5p to identify MYH7 variant carriers with the HCM phenotype with area under the curve value of 0.95 (95% confidence interval: 0.88\u20131.03, p = 0.0004); sensitivity and specificity were 0.86 and 0.91 (cut-off = 0.0014). Therefore, miR-499a-5p could serve as a circulating biomarker of HCM, caused by P\/LP variants in MYH7 gene.<\/jats:p>","DOI":"10.3390\/ijms23073791","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T21:22:14Z","timestamp":1648675334000},"page":"3791","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Circulating miR-499a-5p Is a Potential Biomarker of MYH7\u2014Associated Hypertrophic Cardiomyopathy"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8767-2958","authenticated-orcid":false,"given":"Natalia","family":"Baulina","sequence":"first","affiliation":[{"name":"National Medical Research Center for Cardiology, Laboratory of Functional Genomics of Cardiovascular Diseases, 121552 Moscow, Russia"},{"name":"Laboratory of Medical Genomics, Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Pisklova","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Cardiology, Laboratory of Functional Genomics of Cardiovascular Diseases, 121552 Moscow, Russia"},{"name":"Laboratory of Medical Genomics, Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3366-4113","authenticated-orcid":false,"given":"Ivan","family":"Kiselev","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Cardiology, Laboratory of Functional Genomics of Cardiovascular Diseases, 121552 Moscow, Russia"},{"name":"Laboratory of Medical Genomics, Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2373-1183","authenticated-orcid":false,"given":"Olga","family":"Chumakova","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Cardiology, Laboratory of Functional Genomics of Cardiovascular Diseases, 121552 Moscow, Russia"},{"name":"Moscow Healthcare Department, City Clinical Hospital 17, 119620 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7065-2045","authenticated-orcid":false,"given":"Dmitry","family":"Zateyshchikov","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Cardiology, Laboratory of Functional Genomics of Cardiovascular Diseases, 121552 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Favorova","sequence":"additional","affiliation":[{"name":"National Medical Research Center for Cardiology, Laboratory of Functional Genomics of Cardiovascular Diseases, 121552 Moscow, Russia"},{"name":"Laboratory of Medical Genomics, Pirogov Russian National Research Medical University, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1056\/NEJMra1710575","article-title":"Clinical Course and Management of Hypertrophic Cardiomyopathy","volume":"379","author":"Maron","year":"2018","journal-title":"N. 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