{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:59:45Z","timestamp":1760151585301,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:00:00Z","timestamp":1648598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This research was supported by funds from the Universit\u00e0 degli Studi di Milano (Linea 2, PSR2017_Dozio, Linea 2_PSR2020_Dozio) and the Italian Ministry for Health \u201cRicerca Corrente\u201d I.R.C.C.S. Policlinico San Donato.","award":["N.A."],"award-info":[{"award-number":["N.A."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The etiopathogenesis of obesity-related chronic kidney disease (CKD) is still scarcely understood. To this aim, we assessed the effect of high-fat diet (HF) on molecular pathways leading to organ damage, steatosis, and fibrosis. Six-week-old male C57BL\/6N mice were fed HF diet or normal chow for 20 weeks. Kidneys were collected for genomic, proteomic, histological studies, and lipid quantification. The main findings were as follows: (1) HF diet activated specific pathways leading to fibrosis and increased fatty acid metabolism; (2) HF diet promoted a metabolic shift of lipid metabolism from peroxisomes to mitochondria; (3) no signs of lipid accumulation and\/or fibrosis were observed, histologically; (4) the early signs of kidney damage seemed to be related to changes in membrane protein expression; (5) the proto-oncogene MYC was one of the upstream transcriptional regulators of changes occurring in protein expression. These results demonstrated the potential usefulness of specific selected molecules as early markers of renal injury in HF, while histomorphological changes become visible later in obesity-related CDK. The integration of these information with data from biological fluids could help the identification of biomarkers useful for the early detection and prevention of tissue damage in clinical practice.<\/jats:p>","DOI":"10.3390\/ijms23073809","type":"journal-article","created":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T21:22:14Z","timestamp":1648675334000},"page":"3809","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Wide-Proteome Analysis to Identify Molecular Pathways Involved in Kidney Response to High-Fat Diet in Mice"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5833-0780","authenticated-orcid":false,"given":"Elena","family":"Dozio","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Pathology, Department of Biomedical Sciences for Health, Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5539-1825","authenticated-orcid":false,"given":"Elisa","family":"Maffioli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Vianello","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pathology, Department of Biomedical Sciences for Health, Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3453-7282","authenticated-orcid":false,"given":"Simona","family":"Nonnis","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"},{"name":"CRC \u201cInnovation for Well-Being and Environment\u201d (I-WE), Universit\u00e0 degli Studi di Milano, 29133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Grassi Scalvini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8883-4776","authenticated-orcid":false,"given":"Leonardo","family":"Spatola","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Dialysis and Renal Transplantation, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3672-4612","authenticated-orcid":false,"given":"Paola","family":"Roccabianca","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella","family":"Tedeschi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Science, Universit\u00e0 degli Studi di Milano, 26900 Lodi, Italy"},{"name":"CRC \u201cInnovation for Well-Being and Environment\u201d (I-WE), Universit\u00e0 degli Studi di Milano, 29133 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7928-7697","authenticated-orcid":false,"given":"Massimiliano Marco","family":"Corsi Romanelli","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Pathology, Department of Biomedical Sciences for Health, Universit\u00e0 degli Studi di Milano, 20133 Milan, Italy"},{"name":"Service of Laboratory Medicine1-Clinical Pathology, IRCCS Policlinico San Donato, 20097 San Donato Milanese, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gai, Z., Wang, T., Visentin, M., Kullak-Ublick, G.A., Fu, X., and Wang, Z. 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