{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:58:25Z","timestamp":1760151505329,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009609","name":"Hospital Infantil de M\u00e9xico Federico G\u00f3mez","doi-asserted-by":"publisher","award":["HIM\/2018\/056 SSA 1506 and HIM\/2019\/032 SSA 1602"],"award-info":[{"award-number":["HIM\/2018\/056 SSA 1506 and HIM\/2019\/032 SSA 1602"]}],"id":[{"id":"10.13039\/501100009609","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Myosin 1g (Myo1g) is a mechanoenzyme associated with actin filaments, expressed exclusively in hematopoietic cells, and involved in various cellular functions, including cell migration, adhesion, and membrane trafficking. Despite the importance of Myo1g in distinct functions, there is currently no monoclonal antibody (mAb) against Myo1g. mAbs are helpful tools for the detection of specific antigens in tumor cells and other tissues. The development of mAbs against targeted dysregulated molecules in cancer cells remains a crucial tool for aiding in the diagnosis and the treatment of patients. Using hybridoma technology, we generated a panel of hybridomas specific for Myo1g. ELISA, immunofluorescence, and Western blot assay results revealed the recognition of Myo1g by these novel monoclonal antibodies in normal and transformed T and B cells. Here, we report the development and application of new monoclonal antibodies against Myo1g for their potential use to detect its overexpression in acute lymphoblastic leukemia (ALL) patients.<\/jats:p>","DOI":"10.3390\/ijms23073912","type":"journal-article","created":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T21:22:39Z","timestamp":1648848159000},"page":"3912","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of Myosin 1g Overexpression in Pediatric Leukemia by Novel Monoclonal Antibodies"],"prefix":"10.3390","volume":"23","author":[{"given":"Rosa Isela","family":"Rodr\u00edguez-T\u00e9llez","sequence":"first","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Inmunolog\u00eda y Prote\u00f3mica Hospital Infantil de M\u00e9xico, Federico G\u00f3mez, Mexico City C.P. 06720, Mexico"},{"name":"Laboratorio de Producci\u00f3n y Control de Biol\u00f3gicos, Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Mexico City C.P. 11340, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa Mar\u00eda","family":"Ribas-Aparicio","sequence":"additional","affiliation":[{"name":"Laboratorio de Producci\u00f3n y Control de Biol\u00f3gicos, Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Mexico City C.P. 11340, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8716-722X","authenticated-orcid":false,"given":"Genaro","family":"Pati\u00f1o-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Inmunolog\u00eda y Prote\u00f3mica Hospital Infantil de M\u00e9xico, Federico G\u00f3mez, Mexico City C.P. 06720, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0955-0674(92)90055-H","article-title":"Unconventional myosins","volume":"4","author":"Cheney","year":"1992","journal-title":"Curr. 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