{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T18:33:57Z","timestamp":1772130837514,"version":"3.50.1"},"reference-count":15,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU-H2020 program, SAFE STRIP project","award":["723211"],"award-info":[{"award-number":["723211"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Odorant-binding proteins (OBPs) are a group of small and soluble proteins present in both vertebrates and insects. They have a high level of structural stability and bind to a large spectrum of odorant molecules. In the environmental field, benzene is the most dangerous compound among the class of pollutants named BTEX (benzene, toluene, ethylbenzene, and xylene). It has several effects on human health and, consequently, it appears to be important to monitor its presence in the environment. Commonly, its detection requires the use of very sophisticated and time-consuming analytical techniques (GC-MS, etc.) as well as the presence of specialized personnel. Here, we present the application of an odorant-binding protein (pOBP) isolated from pigs as a molecular recognition element (MRE) for a low-energy impedenziometric biosensor for outdoor and real-time benzene detection. The obtained results show that the biosensor can detect the presence of 64 pM (5 \u00b5g\/m3) benzene, the limit value of exposure for human health set by the European Directive 2008\/50\/EC.<\/jats:p>","DOI":"10.3390\/ijms23074039","type":"journal-article","created":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T16:21:30Z","timestamp":1649262090000},"page":"4039","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Porcine Odorant-Binding Protein as a Probe for an Impedenziometric-Based Detection of Benzene in the Environment"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5566-2867","authenticated-orcid":false,"given":"Alessandro","family":"Capo","sequence":"first","affiliation":[{"name":"Institute of Food Science, CNR Italy, 83100 Avellino, Italy"},{"name":"URT-ISA, CNR at Department of Biology, University of Naples Federico II, 80126 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3528-5344","authenticated-orcid":false,"given":"Serena","family":"Cozzolino","sequence":"additional","affiliation":[{"name":"Institute of Food Science, CNR Italy, 83100 Avellino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolfo","family":"Cavallari","sequence":"additional","affiliation":[{"name":"Megaris, Electronics and Electromechanical Systems, 81100 Caserta, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ugo","family":"Bruno","sequence":"additional","affiliation":[{"name":"Megaris, Electronics and Electromechanical Systems, 81100 Caserta, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8030-4095","authenticated-orcid":false,"given":"Alessia","family":"Calabrese","sequence":"additional","affiliation":[{"name":"Institute of Food Science, CNR Italy, 83100 Avellino, Italy"},{"name":"URT-ISA, CNR at Department of Biology, University of Naples Federico II, 80126 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Pennacchio","sequence":"additional","affiliation":[{"name":"Institute of Food Science, CNR Italy, 83100 Avellino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7546-7527","authenticated-orcid":false,"given":"Alessandra","family":"Camarca","sequence":"additional","affiliation":[{"name":"Institute of Food Science, CNR Italy, 83100 Avellino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Staiano","sequence":"additional","affiliation":[{"name":"Institute of Food Science, CNR Italy, 83100 Avellino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabato","family":"D\u2019Auria","sequence":"additional","affiliation":[{"name":"Institute of Food Science, CNR Italy, 83100 Avellino, Italy"},{"name":"Department of Biology, Agriculture, and Food Sciences, National Research Council of Italy (CNR-DISBA), Piazzale Aldo Moro 7, 00185 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Varriale","sequence":"additional","affiliation":[{"name":"Institute of Food Science, CNR Italy, 83100 Avellino, Italy"},{"name":"URT-ISA, CNR at Department of Biology, University of Naples Federico II, 80126 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj3180001","article-title":"The lipocalin protein family: Structure and function","volume":"318","author":"Flower","year":"1996","journal-title":"Biochem. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/(SICI)1097-4695(199605)30:1<3::AID-NEU2>3.0.CO;2-A","article-title":"Perireceptor events in olfaction","volume":"30","author":"Pelosi","year":"1996","journal-title":"J. Neurobiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"7913","DOI":"10.1021\/bi980179e","article-title":"The Structure of the Monomeric Porcine Odorant Binding Protein Sheds Light on the Domain Swapping Mechanism","volume":"37","author":"Spinelli","year":"1998","journal-title":"Biochemistry"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s00253-013-5383-y","article-title":"Structure and biotechnological applications of odorant-binding proteins","volume":"98","author":"Pelosi","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Capo, A., Pennacchio, A., Varriale, A., D\u2019Auria, S., and Staiano, M. (2018). The porcine odorant-binding protein as molecular probe for benzene detection. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0202630"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Spinelle, L., Gerboles, M., Kok, G., Persijn, S., and Sauerwald, T. (2017). Review of Portable and Low-Cost Sensors for the Ambient Air Monitoring of Benzene and Other Volatile Organic Compounds. Sensors, 17.","DOI":"10.3390\/s17071520"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1021\/sc500106y","article-title":"Impedance Biosensors: Applications to Sustainability and Remaining Technical Challenges","volume":"2","author":"Radhakrishnan","year":"2014","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"9157","DOI":"10.1021\/ac801048g","article-title":"Impedance biosensor for peanut protein Ara h 1","volume":"80","author":"Huang","year":"2008","journal-title":"Anal. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1002\/elan.201300140","article-title":"Detection of Listeria Monocytogenes by Electrochemical Impedance Spectroscopy","volume":"25","author":"Radhakrishnan","year":"2013","journal-title":"Electroanalysis"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.talanta.2009.03.017","article-title":"Interdigitated array microelectrode based impedance immunosensor for detection of avian influenza virus H5N1","volume":"79","author":"Wang","year":"2009","journal-title":"Talanta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3651","DOI":"10.1021\/ac970203e","article-title":"Capacitance measurements of antibody-antigen interactions in a flow system","volume":"69","author":"Berggren","year":"1997","journal-title":"Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/S0956-5663(97)00053-5","article-title":"Capacitive monitoring of protein immobilization and antigen-antibody reactions on monomolecular alkylthiol films on gold electrodes","volume":"12","author":"Mirsky","year":"1997","journal-title":"Biosens. Bioelectron."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1007\/s00216-008-1970-7","article-title":"The use of electrochemical impedance spectroscopy for biosensing","volume":"391","author":"Lisdat","year":"2008","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Cennamo, N., Di Giovanni, S., Varriale, A., Staiano, M., Di Pietrantonio, F., Notargiacomo, A., Zeni, L., and D\u2019Auria, S. (2015). Easy to use plastic optical fiber-based biosensor for detection of butanal. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0116770"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Capone, S., Pascali, C.D., Francioso, L., Siciliano, P., Persaud, K.C., and Pisanelli, A.M. (2009, January 25\u201328). Electrical characterization of a pig odorant binding protein by Impedance Spectroscopy. Proceedings of the SENSORS, 2009 IEEE, Christchurch, New Zealand.","DOI":"10.1109\/ICSENS.2009.5398471"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/7\/4039\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:48:56Z","timestamp":1760136536000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/7\/4039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,6]]},"references-count":15,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,4]]}},"alternative-id":["ijms23074039"],"URL":"https:\/\/doi.org\/10.3390\/ijms23074039","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,6]]}}}