{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T13:16:25Z","timestamp":1792588585873,"version":"4.5.0"},"reference-count":36,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T00:00:00Z","timestamp":1649289600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006041","name":"Innovate UK","doi-asserted-by":"publisher","award":["94851-557155"],"award-info":[{"award-number":["94851-557155"]}],"id":[{"id":"10.13039\/501100006041","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union\u2019s Horizon 2020 research and innovation","award":["734548"],"award-info":[{"award-number":["734548"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mayaro virus (MAYV) is an emerging alphavirus causing acute febrile illness associated with chronic polyarthralgia. Although MAYV is currently restricted to tropical regions in South America around the Amazon basin, it has the potential to spread globally by Aedes species mosquitoes. In addition, there are currently no specific therapeutics or licenced vaccines against MAYV infection. We have previously shown that an adenovirus based Mayaro vaccine (ChAdOx1 May) was able to provide full protection against MAYV challenge in vaccinated A129 mice and induced high neutralising antibody titres. In this study, we have constructed a replication deficient simian adenovirus (ChAdOx2) and a Modified Ankara Virus (MVA) based vaccine expressing the MAYV structural cassette (sMAYV) similar to ChAdOx1 May, and characterised recombinant MAYV E2 glycoprotein expressed in a mammalian system for immune monitoring. We demonstrate that ChAdOx2 May was able to induce high antibody titres similar to ChAdOx1 May, and MVA May was shown to be an effective boosting strategy following prime vaccination with ChAdOx1 or ChAdOx2 May. In order to measure MAYV neutralising ability, we have developed a virus replicon particle-based neutralisation assay which effectively detected neutralising antibodies against MAYV. In summary, our study indicates the potential for further clinical development of the viral vectored MAYV vaccines against MAYV infections.<\/jats:p>","DOI":"10.3390\/ijms23084105","type":"journal-article","created":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T13:39:51Z","timestamp":1649338791000},"page":"4105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of Viral-Vectored Vaccines and Virus Replicon Particle-Based Neutralisation Assay against Mayaro Virus"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2847-9407","authenticated-orcid":false,"given":"Young Chan","family":"Kim","sequence":"first","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, The Henry Wellcome Building for Molecular Physiology, Roosevelt Drive, Oxford OX3 7BN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlen-Celina","family":"L\u00fccke","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Faculty, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7932-2341","authenticated-orcid":false,"given":"C\u00e9sar","family":"L\u00f3pez-Camacho","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, The Henry Wellcome Building for Molecular Physiology, Roosevelt Drive, Oxford OX3 7BN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9011-2764","authenticated-orcid":false,"given":"Beate Mareike","family":"K\u00fcmmerer","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Faculty, University of Bonn, 53127 Bonn, Germany"},{"name":"German Centre for Infection Research (DZIF), Partner Site Bonn-Cologne, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2648-1696","authenticated-orcid":false,"given":"Arturo","family":"Reyes-Sandoval","sequence":"additional","affiliation":[{"name":"The Jenner Institute, Nuffield Department of Medicine, University of Oxford, The Henry Wellcome Building for Molecular Physiology, Roosevelt Drive, Oxford OX3 7BN, UK"},{"name":"Instituto Polit\u00e9cnico Nacional (IPN), Av. Luis Enrique Erro s\/n., Unidad Adolfo L\u00f3pez Mateos, Mexico City 07738, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hotez, P.J., and Murray, K.O. (2017). Dengue, West Nile Virus, Chikungunya, Zika\u2014And Now Mayaro?. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005462"},{"key":"ref_2","first-page":"1","article-title":"Mayaro: An Emerging Viral Threat?","volume":"7","author":"Monsalve","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.3201\/eid1911.130777","article-title":"Mayaro Virus Infection, Amazon Basin Region, Peru, 2010\u20132013","volume":"19","author":"Halsey","year":"2013","journal-title":"Emerging Infect. 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