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Focused sulfonamide-1,2,3-triazole hybrids 3a\u2013c were wisely designed and synthesized via copper catalyzed 1,3-dipolar cycloaddition approach between prop-2-yn-1-alcohol 1 and sulfa drug azides 2a\u2013c. The newly synthesized click products were fully characterized using different spectroscopic experiments and were loaded onto chitosan nanoparticles to form novel nanoformulations for further anti-Toxoplasma investigation. The current study proved the anti-Toxoplasma effectiveness of all examined compounds in experimentally infected mice. Relative to sulfadiazine, the synthesized sulfonamide-1,2,3-triazole (3c) nanoformulae demonstrated the most promising result for toxoplasmosis treatment as it resulted in 100% survival, 100% parasite reduction along with the remarkable histopathological improvement in all the studied organs.<\/jats:p>","DOI":"10.3390\/ijms23084241","type":"journal-article","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T00:23:11Z","timestamp":1649722991000},"page":"4241","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Synthesis, Characterization and Nanoformulation of Novel Sulfonamide-1,2,3-triazole Molecular Conjugates as Potent Antiparasitic Agents"],"prefix":"10.3390","volume":"23","author":[{"given":"Faizah S.","family":"Aljohani","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Science, Taibah University, Al-Madinah Al-Munawarah 30002, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8330-1738","authenticated-orcid":false,"given":"Nadjet","family":"Rezki","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Taibah University, Al-Madinah Al-Munawarah 30002, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7018-6654","authenticated-orcid":false,"given":"Mohamed R.","family":"Aouad","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science, Taibah University, Al-Madinah Al-Munawarah 30002, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-3156","authenticated-orcid":false,"given":"Bassma H.","family":"Elwakil","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria 21321, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0169-7738","authenticated-orcid":false,"given":"Mohamed","family":"Hagar","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Alexandria University, Alexandria 21321, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9018-5229","authenticated-orcid":false,"given":"Eman","family":"Sheta","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, Alexandria University, Alexandria 21321, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nermine Mogahed Fawzy","family":"Hussein Mogahed","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Alexandria University, Alexandria 21321, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4823-0708","authenticated-orcid":false,"given":"Sanaa K.","family":"Bardaweel","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Pharmacy, University of Jordan, Amman 11942, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3185-8504","authenticated-orcid":false,"given":"Nancy Abd-elkader","family":"Hagras","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria 21321, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1017\/S1466252315000183","article-title":"Toxoplasma gondii: History and diagnostic test development","volume":"16","author":"Wyrosdick","year":"2015","journal-title":"Anim. 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