{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T02:38:47Z","timestamp":1785811127362,"version":"3.56.0"},"reference-count":115,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Research, Innovation and Digitalization in Romania","award":["31PFE\/30.12.2021"],"award-info":[{"award-number":["31PFE\/30.12.2021"]}]},{"name":"Ministry of Research, Innovation and Digitalization in Romania","award":["1N\/2016\/ pn16.22.02.05"],"award-info":[{"award-number":["1N\/2016\/ pn16.22.02.05"]}]},{"name":"Ministry of Research, Innovation and Digitalization in Romania","award":["1N\/2019\/PN19.29.01.03"],"award-info":[{"award-number":["1N\/2019\/PN19.29.01.03"]}]},{"name":"Program 1\u2014The Improvement of the National System of Research and Development","award":["31PFE\/30.12.2021"],"award-info":[{"award-number":["31PFE\/30.12.2021"]}]},{"name":"Program 1\u2014The Improvement of the National System of Research and Development","award":["1N\/2016\/ pn16.22.02.05"],"award-info":[{"award-number":["1N\/2016\/ pn16.22.02.05"]}]},{"name":"Program 1\u2014The Improvement of the National System of Research and Development","award":["1N\/2019\/PN19.29.01.03"],"award-info":[{"award-number":["1N\/2019\/PN19.29.01.03"]}]},{"name":"Subprogram 1.2\u2014Institutional Excellence-Projects of Excellence Funding in RDI","award":["31PFE\/30.12.2021"],"award-info":[{"award-number":["31PFE\/30.12.2021"]}]},{"name":"Subprogram 1.2\u2014Institutional Excellence-Projects of Excellence Funding in RDI","award":["1N\/2016\/ pn16.22.02.05"],"award-info":[{"award-number":["1N\/2016\/ pn16.22.02.05"]}]},{"name":"Subprogram 1.2\u2014Institutional Excellence-Projects of Excellence Funding in RDI","award":["1N\/2019\/PN19.29.01.03"],"award-info":[{"award-number":["1N\/2019\/PN19.29.01.03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Although they are considered rare disorders, muscular dystrophies have a strong impact on people\u2019s health. Increased disease severity with age, frequently accompanied by the loss of ability to walk in some people, and the lack of treatment, have directed the researchers towards the development of more effective therapeutic strategies aimed to improve the quality of life and life expectancy, slow down the progression, and delay the onset or convert a severe phenotype into a milder one. Improved understanding of the complex pathology of these diseases together with the tremendous advances in molecular biology technologies has led to personalized therapeutic procedures. Different approaches that are currently under extensive investigation require more efficient, sensitive, and less invasive methods. Due to its remarkable analytical sensitivity, droplet digital PCR has become a promising tool for accurate measurement of biomarkers that monitor disease progression and quantification of various therapeutic efficiency and can be considered a tool for non-invasive prenatal diagnosis and newborn screening. Here, we summarize the recent applications of droplet digital PCR in muscular dystrophy research and discuss the factors that should be considered to get the best performance with this technology.<\/jats:p>","DOI":"10.3390\/ijms23094802","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T13:40:57Z","timestamp":1651066857000},"page":"4802","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Application of Droplet Digital PCR Technology in Muscular Dystrophies Research"],"prefix":"10.3390","volume":"23","author":[{"given":"Ioana","family":"Lambrescu","sequence":"first","affiliation":[{"name":"Laboratory of Cell Biology, Neuroscience and Experimental Myology, Victor Babes National Institute of Pathology, 050096 Bucharest, Romania"},{"name":"Department of Cell Biology and Histology, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Popa","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Neuroscience and Experimental Myology, Victor Babes National Institute of Pathology, 050096 Bucharest, Romania"},{"name":"Department of Animal Production and Public Health, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 050097 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5954-2611","authenticated-orcid":false,"given":"Emilia","family":"Manole","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Neuroscience and Experimental Myology, Victor Babes National Institute of Pathology, 050096 Bucharest, Romania"},{"name":"Pathology Department, Colentina Clinical Hospital, 020125 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Cristina","family":"Ceafalan","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Neuroscience and Experimental Myology, Victor Babes National Institute of Pathology, 050096 Bucharest, Romania"},{"name":"Department of Cell Biology and Histology, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisela","family":"Gaina","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Neuroscience and Experimental Myology, Victor Babes National Institute of Pathology, 050096 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0959-437X(03)00048-0","article-title":"Muscular dystrophies: Genes to pathogenesis","volume":"13","author":"Dalkilic","year":"2003","journal-title":"Curr. 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