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Spoelberch","award":["#ANR-18-IDEX-0001"],"award-info":[{"award-number":["#ANR-18-IDEX-0001"]}]},{"name":"Universit\u00e9 de Paris (Ecole Doctorale BioSPC to CB), the EUR G.E.N.E.","award":["874721\/PREMSTEM"],"award-info":[{"award-number":["874721\/PREMSTEM"]}]},{"name":"Universit\u00e9 de Paris (Ecole Doctorale BioSPC to CB), the EUR G.E.N.E.","award":["Investissement d\u2019Avenir -ANR-11-INBS-0011-"],"award-info":[{"award-number":["Investissement d\u2019Avenir -ANR-11-INBS-0011-"]}]},{"name":"Universit\u00e9 de Paris (Ecole Doctorale BioSPC to CB), the EUR G.E.N.E.","award":["Investissement d\u2019Avenir -ANR-10-INBS-09-"],"award-info":[{"award-number":["Investissement d\u2019Avenir -ANR-10-INBS-09-"]}]},{"name":"Universit\u00e9 de Paris (Ecole Doctorale BioSPC to CB), the EUR G.E.N.E.","award":["#ANR-17-EURE-0013"],"award-info":[{"award-number":["#ANR-17-EURE-0013"]}]},{"name":"Universit\u00e9 de Paris (Ecole Doctorale BioSPC to CB), the EUR G.E.N.E.","award":["#ANR-18-IDEX-0001"],"award-info":[{"award-number":["#ANR-18-IDEX-0001"]}]},{"name":"French Government through its \u201cInvestments for the Future\u201d program","award":["874721\/PREMSTEM"],"award-info":[{"award-number":["874721\/PREMSTEM"]}]},{"name":"French Government through its \u201cInvestments for the Future\u201d program","award":["Investissement d\u2019Avenir -ANR-11-INBS-0011-"],"award-info":[{"award-number":["Investissement d\u2019Avenir -ANR-11-INBS-0011-"]}]},{"name":"French Government through its \u201cInvestments for the Future\u201d program","award":["Investissement d\u2019Avenir -ANR-10-INBS-09-"],"award-info":[{"award-number":["Investissement d\u2019Avenir -ANR-10-INBS-09-"]}]},{"name":"French Government through its \u201cInvestments for the Future\u201d program","award":["#ANR-17-EURE-0013"],"award-info":[{"award-number":["#ANR-17-EURE-0013"]}]},{"name":"French Government through its \u201cInvestments for the Future\u201d program","award":["#ANR-18-IDEX-0001"],"award-info":[{"award-number":["#ANR-18-IDEX-0001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Preterm birth (PTB) represents 15 million births every year worldwide and is frequently associated with maternal\/fetal infections and inflammation, inducing neuroinflammation. This neuroinflammation is mediated by microglial cells, which are brain-resident macrophages that release cytotoxic molecules that block oligodendrocyte differentiation, leading to hypomyelination. Some preterm survivors can face lifetime motor and\/or cognitive disabilities linked to periventricular white matter injuries (PWMIs). There is currently no recommendation concerning the mode of delivery in the case of PTB and its impact on brain development. Many animal models of induced-PTB based on LPS injections exist, but with a low survival rate. There is a lack of information regarding clinically used pharmacological substances to induce PTB and their consequences on brain development. Mifepristone (RU-486) is a drug used clinically to induce preterm labor. This study aims to elaborate and characterize a new model of induced-PTB and PWMIs by the gestational injection of RU-486 and the perinatal injection of pups with IL-1beta. A RU-486 single subcutaneous (s.c.) injection at embryonic day (E)18.5 induced PTB at E19.5 in pregnant OF1 mice. All pups were born alive and were adopted directly after birth. IL-1beta was injected intraperitoneally from postnatal day (P)1 to P5. Animals exposed to both RU-486 and IL-1beta demonstrated microglial reactivity and subsequent PWMIs. In conclusion, the s.c. administration of RU-486 induced labor within 24 h with a high survival rate for pups. In the context of perinatal inflammation, RU-486 labor induction significantly decreases microglial reactivity in vivo but did not prevent subsequent PWMIs.<\/jats:p>","DOI":"10.3390\/ijms23094867","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T22:20:20Z","timestamp":1651098020000},"page":"4867","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Impact of Mouse Preterm Birth Induction by RU-486 on Microglial Activation and Subsequent Hypomyelination"],"prefix":"10.3390","volume":"23","author":[{"given":"C\u00e9cile","family":"Morin","sequence":"first","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"},{"name":"AP-HP, H\u00f4pital Robert Debr\u00e9, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8377-4703","authenticated-orcid":false,"given":"David","family":"Guenoun","sequence":"additional","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"},{"name":"AP-HP, H\u00f4pital Robert Debr\u00e9, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irvin","family":"Sautet","sequence":"additional","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Faivre","sequence":"additional","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsolt","family":"Csaba","sequence":"additional","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie","family":"Schwendimann","sequence":"additional","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierrette","family":"Young-Ten","sequence":"additional","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliette","family":"Van Steenwinckel","sequence":"additional","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0909-4221","authenticated-orcid":false,"given":"Pierre","family":"Gressens","sequence":"additional","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0992-4317","authenticated-orcid":false,"given":"Cindy","family":"Bokobza","sequence":"additional","affiliation":[{"name":"NeuroDiderot, Inserm UMR-1141, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 de Paris, 75019 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"n741","DOI":"10.1136\/bmj.n741","article-title":"Neurodevelopmental outcomes at age 5 among children born preterm: EPIPAGE-2 cohort study","volume":"373","author":"Pierrat","year":"2021","journal-title":"BMJ"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.nbd.2015.10.012","article-title":"Controversies in preterm brain injury","volume":"92","author":"Penn","year":"2015","journal-title":"Neurobiol. 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