{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:46:51Z","timestamp":1791895611484,"version":"4.3.4"},"reference-count":69,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T00:00:00Z","timestamp":1651536000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Association de Recherche en Virologie et Dermatologie"},{"name":"French Society for Dermatology (SFD)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cutibacterium acnes (C. acnes) has been implicated in inflammatory acne where highly mutated Christie\u2013Atkins\u2013Munch\u2013Petersen factor (CAMP)1 displays strong toll like receptor (TLR)-2 binding activity. Using specific antibodies, we showed that CAMP1 production was independent of C. acnes phylotype and involved in the induction of inflammation. We confirmed that TLR-2 bound both mutated and non-mutated recombinant CAMP1, and peptide array analysis showed that seven peptides (A14, A15, B1, B2, B3, C1 and C3) were involved in TLR-2 binding, located on the same side of the three-dimensional structure of CAMP1. Both mutated and non-mutated recombinant CAMP1 proteins induced the production of C-X-C motif chemokine ligand interleukin (CXCL)8\/(IL)-8 in vitro in keratinocytes and that of granulocyte macrophage-colony stimulating factor (GM-CSF), tumor necrosis factor (TNF)-\u03b1, IL-1\u03b2 and IL-10 in ex vivo human skin explants. Only A14, B1 and B2 inhibited the production of CXCL8\/IL-8 by keratinocytes and that of (GM-CSF), TNF-\u03b1, IL-1\u03b2 and IL-10 in human skin explants stimulated with rCAMP1 and C. acnes. Following pretreatment with B2, RNA sequencing on skin explants identified the 10 genes displaying the strongest differential expression as IL6, TNF, CXCL1, CXCL2, CXCL3, CXCL8, IL-1\u03b2, chemokine ligand (CCL)2, CCL4 and colony stimulating factor (CSF)2. We, thus, identified a new CAMP1-derived peptide as a TLR-2 modulator likely to be a good candidate for clinical evaluation.<\/jats:p>","DOI":"10.3390\/ijms23095065","type":"journal-article","created":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T08:26:35Z","timestamp":1651566395000},"page":"5065","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7789-5090","authenticated-orcid":false,"given":"Constance","family":"Mayslich","sequence":"first","affiliation":[{"name":"D\u00e9partement DRC, D\u00e9veloppement, Reproduction et Cancer, Institut Cochin, INSERM U1016-CNRS UMR8104, Universit\u00e9 Paris Cit\u00e9, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8151-748X","authenticated-orcid":false,"given":"Philippe Alain","family":"Grange","sequence":"additional","affiliation":[{"name":"D\u00e9partement DRC, D\u00e9veloppement, Reproduction et Cancer, Institut Cochin, INSERM U1016-CNRS UMR8104, Universit\u00e9 Paris Cit\u00e9, 75014 Paris, France"},{"name":"Service de Dermatologie-V\u00e9n\u00e9r\u00e9ologie et CeGIDD, Groupe Hospitalier APHP.centre, CNR IST Bact\u00e9riennes\u2014Laboratoire Associ\u00e9 Syphilis, 75014 Paris, France"},{"name":"H\u00f4pital Cochin, U1016, Equipe Biologie Cutan\u00e9e\u2014CNR IST bact\u00e9riennes\u2014Syphilis 24, rue du faubourg Saint-Jacques, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8745-9583","authenticated-orcid":false,"given":"Mathieu","family":"Castela","sequence":"additional","affiliation":[{"name":"D\u00e9partement DRC, D\u00e9veloppement, Reproduction et Cancer, Institut Cochin, INSERM U1016-CNRS UMR8104, Universit\u00e9 Paris Cit\u00e9, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4808-8999","authenticated-orcid":false,"given":"Anne Genevi\u00e8ve","family":"Marcelin","sequence":"additional","affiliation":[{"name":"National Reference Centre for Herpesviruses, Virology Department, Team 3 THERAVIR, and AP-HP, Piti\u00e9-Salp\u00eatri\u00e8re\u2014Charles Foix University Hospital, Institut Pierre Louis d\u2019Epid\u00e9miologie et de Sant\u00e9 Publique (iPLESP), INSERM, Sorbonne Universit\u00e9, 75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Calvez","sequence":"additional","affiliation":[{"name":"National Reference Centre for Herpesviruses, Virology Department, Team 3 THERAVIR, and AP-HP, Piti\u00e9-Salp\u00eatri\u00e8re\u2014Charles Foix University Hospital, Institut Pierre Louis d\u2019Epid\u00e9miologie et de Sant\u00e9 Publique (iPLESP), INSERM, Sorbonne Universit\u00e9, 75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Dupin","sequence":"additional","affiliation":[{"name":"D\u00e9partement DRC, D\u00e9veloppement, Reproduction et Cancer, Institut Cochin, INSERM U1016-CNRS UMR8104, Universit\u00e9 Paris Cit\u00e9, 75014 Paris, France"},{"name":"Service de Dermatologie-V\u00e9n\u00e9r\u00e9ologie et CeGIDD, Groupe Hospitalier APHP.centre, CNR IST Bact\u00e9riennes\u2014Laboratoire Associ\u00e9 Syphilis, 75014 Paris, France"},{"name":"H\u00f4pital Cochin, U1016, Equipe Biologie Cutan\u00e9e\u2014CNR IST bact\u00e9riennes\u2014Syphilis 24, rue du faubourg Saint-Jacques, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4422","DOI":"10.1099\/ijsem.0.001367","article-title":"The natural history of cutaneous propionibacteria, and reclassification of selected species within the genus Propionibacterium to the proposed novel genera Acidipropionibacterium gen. nov., Cutibacterium gen. nov. and Pseudopropionibacterium gen. nov","volume":"66","author":"Scholz","year":"2016","journal-title":"Int. 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