{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:48:38Z","timestamp":1782236918880,"version":"3.54.5"},"reference-count":99,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T00:00:00Z","timestamp":1651795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In addition to the classical oestrogen receptors, ER\u03b1 and ER\u03b2, a G protein-coupled oestrogen receptor (GPER) has been identified that primarily mediates the rapid, non-genomic signalling of oestrogens. Data on GPER expression at the protein level are contradictory; therefore, the present study was conducted to re-evaluate GPER expression by immunohistochemistry to obtain broad GPER expression profiles in human non-neoplastic and neoplastic tissues, especially those not investigated in this respect so far. We developed and thoroughly characterised a novel rabbit monoclonal anti-human GPER antibody, 20H15L21, using Western blot analyses and immunocytochemistry. The antibody was then applied to a large series of formalin-fixed, paraffin-embedded human tissue samples. In normal tissue, GPER was identified in distinct cell populations of the cortex and the anterior pituitary; islets and pancreatic ducts; fundic glands of the stomach; the epithelium of the duodenum and gallbladder; hepatocytes; proximal tubules of the kidney; the adrenal medulla; and syncytiotrophoblasts and decidua cells of the placenta. GPER was also expressed in hepatocellular, pancreatic, renal, and endometrial cancers, pancreatic neuroendocrine tumours, and pheochromocytomas. The novel antibody 20H15L21 will serve as a valuable tool for basic research and the identification of GPER-expressing tumours during histopathological examinations.<\/jats:p>","DOI":"10.3390\/ijms23095191","type":"journal-article","created":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T14:49:38Z","timestamp":1651848578000},"page":"5191","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Assessment of G Protein-Coupled Oestrogen Receptor Expression in Normal and Neoplastic Human Tissues Using a Novel Rabbit Monoclonal Antibody"],"prefix":"10.3390","volume":"23","author":[{"given":"Maria","family":"Bubb","sequence":"first","affiliation":[{"name":"Institute of Pharmacology and Toxicology, Jena University Hospital, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna-Sophia Lieselott","family":"Beyer","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, Jena University Hospital, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6369-122X","authenticated-orcid":false,"given":"Pooja","family":"Dasgupta","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, Jena University Hospital, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Kaemmerer","sequence":"additional","affiliation":[{"name":"Department of General and Visceral Surgery, Zentralklinik Bad Berka, 99438 Bad Berka, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"S\u00e4nger","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology and Cytology Bad Berka, 99438 Bad Berka, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Evert","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Regensburg, 93053 Regensburg, Germany"},{"name":"Institute of Pathology, University Medicine of Greifswald, 17475 Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph M.","family":"Wirtz","sequence":"additional","affiliation":[{"name":"STRATIFYER Molecular Pathology GmbH, 50935 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5997-8885","authenticated-orcid":false,"given":"Stefan","family":"Schulz","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, Jena University Hospital, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0141-7310","authenticated-orcid":false,"given":"Amelie","family":"Lupp","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, Jena University Hospital, 07747 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1124\/pr.114.009712","article-title":"International Union of Basic and Clinical Pharmacology. 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