{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:33:44Z","timestamp":1787315624113,"version":"build-2736575974"},"reference-count":80,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Science and Innovation","award":["PID2019-103924GB-I00"],"award-info":[{"award-number":["PID2019-103924GB-I00"]}]},{"name":"Spanish Ministry of Science and Innovation","award":["P18-FR-1359"],"award-info":[{"award-number":["P18-FR-1359"]}]},{"name":"Junta de Andaluc\u00eda","award":["PID2019-103924GB-I00"],"award-info":[{"award-number":["PID2019-103924GB-I00"]}]},{"name":"Junta de Andaluc\u00eda","award":["P18-FR-1359"],"award-info":[{"award-number":["P18-FR-1359"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>H2S has acquired great attention in plant research because it has signaling functions under physiological and stress conditions. However, the direct detection of endogenous H2S and its potential emission is still a challenge in higher plants. In order to achieve a comparative analysis of the content of H2S among different plants with agronomical and nutritional interest including pepper fruits, broccoli, ginger, and different members of the genus Allium such as garlic, leek, Welsh and purple onion, the endogenous H2S and its emission was determined using an ion-selective microelectrode and a specific gas detector, respectively. The data show that endogenous H2S content range from pmol to \u03bcmol H2S \u00b7 g\u22121 fresh weight whereas the H2S emission of fresh-cut vegetables was only detected in the different species of the genus Allium with a maximum of 9 ppm in garlic cloves. Additionally, the activity and isozymes of the L-cysteine desulfhydrase (LCD) were analyzed, which is one of the main enzymatic sources of H2S, where the different species of the genus Allium showed the highest activities. Using non-denaturing gel electrophoresis, the data indicated the presence of up to nine different LCD isozymes from one in ginger to four in onion, leek, and broccoli. In summary, the data indicate a correlation between higher LCD activity with the endogenous H2S content and its emission in the analyzed horticultural species. Furthermore, the high content of endogenous H2S in the Allium species supports the recognized benefits for human health, which are associated with its consumption.<\/jats:p>","DOI":"10.3390\/ijms23105648","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T11:59:37Z","timestamp":1652875177000},"page":"5648","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["H2S in Horticultural Plants: Endogenous Detection by an Electrochemical Sensor, Emission by a Gas Detector, and Its Correlation with L-Cysteine Desulfhydrase (LCD) Activity"],"prefix":"10.3390","volume":"23","author":[{"given":"Mar\u00eda A.","family":"Mu\u00f1oz-Vargas","sequence":"first","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estaci\u00f3n Experimental del Zaid\u00edn, Spanish National Research Council (CSIC), C\/Profesor Albareda 1, E-18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3678-5503","authenticated-orcid":false,"given":"Salvador","family":"Gonz\u00e1lez-Gordo","sequence":"additional","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estaci\u00f3n Experimental del Zaid\u00edn, Spanish National Research Council (CSIC), C\/Profesor Albareda 1, E-18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6673-3571","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"Palma","sequence":"additional","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estaci\u00f3n Experimental del Zaid\u00edn, Spanish National Research Council (CSIC), C\/Profesor Albareda 1, E-18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1814-9212","authenticated-orcid":false,"given":"Francisco J.","family":"Corpas","sequence":"additional","affiliation":[{"name":"Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estaci\u00f3n Experimental del Zaid\u00edn, Spanish National Research Council (CSIC), C\/Profesor Albareda 1, E-18008 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.tibs.2008.06.006","article-title":"The undertow of sulfur metabolism on glutamatergic neurotransmission","volume":"33","author":"Banerjee","year":"2008","journal-title":"Trends Biochem. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1146\/annurev-nutr-071813-105654","article-title":"Sulfur as a signaling nutrient through hydrogen sulfide","volume":"34","author":"Kabil","year":"2014","journal-title":"Annu Rev. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Szabo, C. (2021). Hydrogen sulfide, an endogenous stimulator of mitochondrial function in cancer cells. Cells, 10.","DOI":"10.3390\/cells10020220"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1177\/0271678X19878284","article-title":"Vasodilator effects of sulforaphane in cerebral circulation: A critical role of endogenously produced hydrogen sulfide and arteriolar smooth muscle KATP and BK channels in the brain","volume":"40","author":"Parfenova","year":"2020","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1080\/10409238.2021.1893641","article-title":"Regulation of the redox metabolome and thiol proteome by hydrogen sulfide","volume":"56","author":"Kumar","year":"2021","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kimura, H. (2021). Hydrogen sulfide (H2S) and polysulfide (H2Sn) signaling: The First 25 Years. Biomolecules, 11.","DOI":"10.3390\/biom11060896"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Deng, G., Muqadas, M., Adlat, S., Zheng, H., Li, G., Zhu, P., and Nasser, M.I. Protective effect of hydrogen sulfide on cerebral ischemia-reperfusion injury. Cell. Mol. Neurobiol., 2022. in press.","DOI":"10.1007\/s10571-021-01166-4"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4391","DOI":"10.1093\/jxb\/erz031","article-title":"Nitric oxide and hydrogen sulfide in plants: Which comes first?","volume":"70","author":"Corpas","year":"2019","journal-title":"J. Exp. Bot."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.1016\/j.tplants.2021.07.016","article-title":"Nitric oxide and hydrogen sulfide: An indispensable combination for plant functioning","volume":"26","author":"Mishra","year":"2021","journal-title":"Trends Plant Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"124907","DOI":"10.1016\/j.jhazmat.2020.124907","article-title":"Hydrogen sulfide (H2S) underpins the beneficial silicon effects against the copper oxide nanoparticles (CuO NPs) phytotoxicity in Oryza sativa seedlings","volume":"415","author":"Rai","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Raza, A., Tabassum, J., Mubarik, M.S., Anwar, S., Zahra, N., Sharif, Y., Hafeez, M.B., Zhang, C., Corpas, F.J., and Chen, H. Hydrogen sulfide: An emerging component against abiotic stress in plants. Plant Biol., 2021. in press.","DOI":"10.1111\/plb.13368"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/jipb.13022","article-title":"Hydrogen sulfide, a signaling molecule in plant stress responses","volume":"63","author":"Zhang","year":"2021","journal-title":"J. Integr. Plant Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5893","DOI":"10.1093\/jxb\/erab239","article-title":"Hydrogen sulfide signaling in plant adaptations to adverse conditions: Molecular mechanisms","volume":"72","author":"Aroca","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.plaphy.2020.08.014","article-title":"Appraisal of H2S metabolism in Arabidopsis thaliana: In silico analysis at the subcellular level","volume":"155","author":"Palma","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1021\/acs.chemrev.7b00205","article-title":"Chemical biology of H2S signaling through persulfidation","volume":"118","author":"Filipovic","year":"2018","journal-title":"Chem. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Corpas, F.J., Gonz\u00e1lez-Gordo, S., Mu\u00f1oz-Vargas, M.A., Rodr\u00edguez-Ruiz, M., and Palma, J.M. (2021). The modus operandi of hydrogen sulfide(H2S)-dependent protein persulfidation in higher plants. Antioxidants, 10.","DOI":"10.3390\/antiox10111686"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1093\/jxb\/eraa440","article-title":"Nitric oxide and hydrogen sulfide modulate the NADPH-generating enzymatic system in higher plants","volume":"72","author":"Corpas","year":"2021","journal-title":"J. Exp. Bot."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.plaphy.2020.08.006","article-title":"Current approaches for detection of hydrogen sulfide and persulfidation in biological systems","volume":"155","author":"Zhao","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2809","DOI":"10.1007\/s00216-021-03777-8","article-title":"Progress on the reaction-based methods for detection of endogenous hydrogen sulfide","volume":"414","author":"Luo","year":"2021","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5473965","DOI":"10.1155\/2021\/5473965","article-title":"Analytical methods for detection of gasotransmitter hydrogen sulfide released from live cells","volume":"2021","author":"Quan","year":"2021","journal-title":"BioMed Res. Int."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1089\/ars.2021.0195","article-title":"Chemiluminescence measurement of reactive sulfur and nitrogen species","volume":"36","author":"Li","year":"2022","journal-title":"Antioxid. Redox Signal."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1111\/nph.15704","article-title":"Distribution of control in the sulfur assimilation in Arabidopsis thaliana depends on environmental conditions","volume":"222","author":"Veith","year":"2019","journal-title":"New Phytol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1093\/jxb\/erac021","article-title":"Active O-acetylserine-(thiol) lyase A and B confer improved selenium resistance and degrade l-Cys and l-SeCys in Arabidopsis","volume":"73","author":"Kurmanbayeva","year":"2022","journal-title":"J. Exp. Bot."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Cou\u00e9e, I. (2023). Analysis of Plant L-Cysteine desulfhydrase (LCD) isozymes by non-denaturing polyacrylamide gel electrophoresis. Plant Abiotic Stress Signaling. Methods in Molecular Biology, Humana. in press.","DOI":"10.1007\/978-1-0716-3044-0"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s11103-018-00817-3","article-title":"L-Cysteine desulfhydrase-dependent hydrogen sulfide is required for methane-induced lateral root formation","volume":"99","author":"Mei","year":"2019","journal-title":"Plant Mol. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1104\/pp.114.245373","article-title":"Hydrogen sulfide generated by L-cysteine desulfhydrase acts upstream of nitric oxide to modulate abscisic acid-dependent stomatal closure","volume":"166","author":"Scuffi","year":"2014","journal-title":"Plant Physiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1105\/tpc.19.00826","article-title":"Persulfidation-based modification of cysteine desulfhydrase and the NADPH oxidase RBOHD controls guard cell abscisic acid signaling","volume":"32","author":"Shen","year":"2020","journal-title":"Plant Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1071\/FP20205","article-title":"Hydrogen sulfide induced by hydrogen peroxide mediates brassinosteroid-induced stomatal closure of Arabidopsis thaliana","volume":"48","author":"Ma","year":"2021","journal-title":"Funct. Plant Biol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Hu, K., Peng, X., Yao, G., Zhou, Z., Yang, F., Li, W., Zhao, Y., Li, Y., Han, Z., and Chen, X. (2021). Roles of a cysteine desulfhydrase LCD1 in regulating leaf senescence in tomato. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222313078"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.niox.2018.10.002","article-title":"Endogenous hydrogen sulfide (H2S) is up-regulated during sweet pepper (Capsicum annuum L.) fruit ripening. In vitro analysis shows that NADP-dependent isocitrate dehydrogenase (ICDH) activity is inhibited by H2S and NO","volume":"81","author":"Palma","year":"2018","journal-title":"Nitric Oxide"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/s41438-020-00439-1","article-title":"A nuclear-localized cysteine desulfhydrase plays a role in fruit ripening in tomato","volume":"7","author":"Hu","year":"2020","journal-title":"Hortic. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"127302","DOI":"10.1016\/j.jhazmat.2021.127302","article-title":"Nitric oxide donor, sodium nitroprusside modulates hydrogen sulfide metabolism and cysteine homeostasis to aid the alleviation of chromium toxicity in maize seedlings (Zea mays L.)","volume":"424 Pt A","author":"Kharbech","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.bcp.2018.01.017","article-title":"Regulation of vascular tone homeostasis by NO and H2S: Implications in hypertension","volume":"149","author":"Gheibi","year":"2018","journal-title":"Biochem. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jare.2020.05.007","article-title":"Hydrogen sulfide and vascular regulation\u2014An update","volume":"27","author":"Lv","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1007\/s00702-016-1600-z","article-title":"Hydrogen polysulfide (H2Sn) signaling along with hydrogen sulfide (H2S) and nitric oxide (NO)","volume":"123","author":"Kimura","year":"2016","journal-title":"J. Neural Transm. (Vienna)"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5031","DOI":"10.1111\/bph.15246","article-title":"Hydrogen sulfide signalling in the CNS\u2014Comparison with NO","volume":"177","author":"Kimura","year":"2020","journal-title":"Br. J. Pharmacol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.tplants.2019.08.003","article-title":"Hydrogen sulfide: A new warrior against abiotic Stress","volume":"24","author":"Corpas","year":"2019","journal-title":"Trends Plant Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.plaphy.2020.10.017","article-title":"Nitric oxide and hydrogen sulfide protect plasma membrane integrity and mitigate chromium-induced methylglyoxal toxicity in maize seedlings","volume":"157","author":"Kharbech","year":"2020","journal-title":"Plant Physiol. Biochem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1055\/s-2007-965424","article-title":"Characterization of cysteine-degrading and H2S-releasing enzymes of higher plants\u2014From the field to the test tube and back","volume":"9","author":"Papenbrock","year":"2007","journal-title":"Plant Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1002\/chem.201303757","article-title":"Fluorescent probes based on nucleophilic substitution-cyclization for hydrogen sulfide detection and bioimaging","volume":"20","author":"Peng","year":"2014","journal-title":"Chemistry"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"12234","DOI":"10.1039\/C4CC03312D","article-title":"Fluorescent probes for hydrogen sulfide detection and bioimaging","volume":"50","author":"Yu","year":"2014","journal-title":"Chem. Commun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1111\/jipb.12779","article-title":"Hydrogen sulfide: A novel component in Arabidopsis peroxisomes which triggers catalase inhibition","volume":"61","author":"Corpas","year":"2019","journal-title":"J. Integr. Plant Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"14330","DOI":"10.1021\/acs.jafc.1c05247","article-title":"NIR Fluorescent probe for in situ bioimaging of endogenous H2S in rice roots under Al3+ and flooding stresses","volume":"69","author":"Wang","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1104\/pp.61.2.184","article-title":"Light-dependent emission of hydrogen sulfide from plants","volume":"61","author":"Wilson","year":"1978","journal-title":"Plant Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1038\/289672a0","article-title":"Rates of emission of H2S from plants and patterns of stable sulphur isotope fractionation","volume":"289","author":"Winner","year":"1981","journal-title":"Nature"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1111\/j.1469-8137.2010.03465.x","article-title":"Hydrogen sulphide, a novel gasotransmitter involved in guard cell signaling","volume":"188","author":"Lamattina","year":"2010","journal-title":"New Phytol."},{"key":"ref_47","first-page":"396","article-title":"H2S may function downstream of H2O2 in jasmonic acid-induced stomatal closure in Vicia faba","volume":"4","author":"Hou","year":"2011","journal-title":"Chin. Bull. Bot."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1016\/j.scib.2018.09.012","article-title":"Diversity of hydrogen sulfide concentration in plant: A little spark to start a prairie fire","volume":"63","author":"Jin","year":"2018","journal-title":"Sci. Bull."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1104\/pp.70.2.430","article-title":"Emission of hydrogen sulfide by leaf tissue in response to L-cysteine","volume":"70","author":"Sekiya","year":"1982","journal-title":"Plant Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1104\/pp.73.3.560","article-title":"Role of o-acetylserine in hydrogen sulfide emission from pumpkin leaves in response to sulfate","volume":"73","author":"Rennenberg","year":"1983","journal-title":"Plant Physiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3753","DOI":"10.1021\/jf990521+","article-title":"Cysteine sulfoxides and alliinase activity of some Allium species","volume":"48","author":"Krest","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4123","DOI":"10.1093\/jxb\/erz243","article-title":"S-Alk(en)ylcysteine sulfoxides in the genus Allium: Proposed biosynthesis, chemical conversion, and bioactivities","volume":"70","author":"Yoshimoto","year":"2019","journal-title":"J. Exp. Bot."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0304-4211(81)90139-5","article-title":"1981. Presence of alliinase in isolated vacuoles and of alkyl cysteine sulphoxides in the cytoplasm of bulbs of onion (Allium cepa)","volume":"22","author":"Lancaster","year":"1981","journal-title":"Plant Sci. Lett."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.1016\/0031-9422(88)80108-0","article-title":"Production of flavor precursors [S-alk(en)yl-l-cysteine sulphoxides] in photomixotrophic callus of garlic","volume":"27","author":"Lancaster","year":"1988","journal-title":"Phytochemistry"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/0031-9422(89)80032-9","article-title":"Intra-cellular localization of the biosynthetic pathway to flavour precursors in onion","volume":"28","author":"Lancaster","year":"1989","journal-title":"Phytochemistry"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s11418-021-01533-x","article-title":"Thiolane-type sulfides from garlic, onion, and Welsh onion","volume":"75","author":"Nohara","year":"2021","journal-title":"J. Nat. Med."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1475-2891-1-4","article-title":"Effect of garlic on cardiovascular disorders: A review","volume":"1","author":"Banerjee","year":"2002","journal-title":"Nutr. J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"17977","DOI":"10.1073\/pnas.0705710104","article-title":"Hydrogen sulfide mediates the vasoactivity of garlic","volume":"104","author":"Benavides","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/j.tips.2018.03.009","article-title":"Garlic and Gaseous Mediators","volume":"39","author":"Rose","year":"2018","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.2174\/1381612822666161010124530","article-title":"Garlic for cardiovascular disease: Prevention or treatment?","volume":"23","author":"Alali","year":"2017","journal-title":"Curr. Pharm. Des."},{"key":"ref_61","first-page":"395","article-title":"Sulfur and sulfur compounds in plant defence","volume":"7","author":"Nwachukwu","year":"2012","journal-title":"Nat. Prod. Commun."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"K\u00fcnstler, A., Gullner, G., \u00c1d\u00e1m, A.L., Kolozsv\u00e1rin\u00e9 Nagy, J., and Kir\u00e1ly, L. (2020). The versatile roles of sulfur-containing biomolecules in plant defense-a road to disease resistance. Plants, 9.","DOI":"10.3390\/plants9121705"},{"key":"ref_63","first-page":"683","article-title":"Assessing the transcriptional regulation of L-cysteine desulfhydrase 1 in Arabidopsis thaliana","volume":"5","author":"Romero","year":"2014","journal-title":"Front. Plant Sci."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Zhou, M., Zhang, J., Zhou, H., Zhao, D., Duan, T., Wang, S., Yuan, X., and Xie, Y. (2022). Hydrogen sulfide-linked persulfidation maintains protein stability of ABSCISIC ACID-INSENSITIVE 4 and delays seed germination. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031389"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.plaphy.2013.12.024","article-title":"Auxin-induced hydrogen sulfide generation is involved in lateral root formation in tomato","volume":"76","author":"Fang","year":"2014","journal-title":"Plant Physiol. Biochem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1111\/pce.13685","article-title":"Abscisic acid-triggered guard cell l-cysteine desulfhydrase function and in situ hydrogen sulfide production contributes to heme oxygenase-modulated stomatal closure","volume":"43","author":"Zhang","year":"2020","journal-title":"Plant Cell Environ."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Zhou, H., Zhou, Y., Zhang, F., Guan, W., Su, Y., Yuan, X., and Xie, Y. (2021). Persulfidation of nitrate reductase 2 is involved in l-cysteine desulfhydrase-regulated rice drought tolerance. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222212119"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1111\/ppl.13000","article-title":"Inhibition of NADP-malic enzyme activity by H2 S and NO in sweet pepper (Capsicum annuum L.) fruits","volume":"168","author":"Palma","year":"2020","journal-title":"Physiol. Plant."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1104\/pp.104.057125","article-title":"Impact of reduced O-acetylserine(thiol)lyase isoform contents on potato plant metabolism","volume":"137","author":"Riemenschneider","year":"2005","journal-title":"Plant Physiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1104\/pp.109.147975","article-title":"An O-acetylserine(thiol)lyase homolog with L-cysteine desulfhydrase activity regulates cysteine homeostasis in Arabidopsis","volume":"152","author":"Alvarez","year":"2010","journal-title":"Plant Physiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0003-2697(75)90213-4","article-title":"Activity staining of cystathionine-beta-synthetase and related enzymes","volume":"63","author":"Willhardt","year":"1975","journal-title":"Anal. Biochem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.niox.2014.02.005","article-title":"Hydrogen sulfide in plants: From dissipation of excess sulfur to signaling molecule","volume":"41","author":"Calderwood","year":"2014","journal-title":"Nitric Oxide"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"152","DOI":"10.21273\/HORTSCI.51.2.152","article-title":"Hydrogen sulfide alleviates senescence of fresh-cut apple by regulating antioxidant defense system and senescence-related gene expression","volume":"51","author":"Zheng","year":"2016","journal-title":"HortScience"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Ge, Y., Hu, K.D., Wang, S.S., Hu, L.Y., Chen, X.Y., and Li, Y.H. (2017). Hydrogen sulfide alleviates postharvest ripening and senescence of banana by antagonizing the effect of ethylene. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0180113"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.3389\/fpls.2018.01172","article-title":"Hydrogen sulfide: A gaseous molecule in postharvest freshness","volume":"9","author":"Huo","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jare.2020.03.011","article-title":"H2S signaling in plants and applications in agriculture","volume":"24","author":"Corpas","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Molinett, S.A., Alfaro, J.F., S\u00e1ez, F.A., Elgueta, S., Moya-Le\u00f3n, M.A., and Figueroa, C.R. (2021). Postharvest treatment of hydrogen sulfide delays the softening of chilean strawberry fruit by downregulating the expression of key genes involved in pectin catabolism. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221810008"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Siddiqui, M.W., Homa, F., Lata, D., Mir, H., Aftab, T., and Mishra, P. Hydrogen sulphide infiltration downregulates oxidative metabolism and extends postharvest life of banana. Plant Biol., 2021. in press.","DOI":"10.1111\/plb.13362"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Rose, P., Moore, P.K., Whiteman, M., and Zhu, Y.Z. (2019). An appraisal of developments in Allium sulfur chemistry: Expanding the pharmacopeia of garlic. Molecules, 24.","DOI":"10.3390\/molecules24214006"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Piragine, E., Citi, V., Lawson, K., Calderone, V., and Martelli, A. (2022). Potential effects of natural H2S-donors in hypertension management. Biomolecules, 12.","DOI":"10.3390\/biom12040581"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/10\/5648\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:14:23Z","timestamp":1760138063000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/23\/10\/5648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,18]]},"references-count":80,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["ijms23105648"],"URL":"https:\/\/doi.org\/10.3390\/ijms23105648","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,18]]}}}