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Technology Program of Gansu Province of China","award":["NWNU-LKQN2019-21"],"award-info":[{"award-number":["NWNU-LKQN2019-21"]}]},{"name":"the Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University","award":["XDA24010402"],"award-info":[{"award-number":["XDA24010402"]}]},{"name":"the Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University","award":["32160438"],"award-info":[{"award-number":["32160438"]}]},{"name":"the Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University","award":["61972374"],"award-info":[{"award-number":["61972374"]}]},{"name":"the Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University","award":["2021A-007"],"award-info":[{"award-number":["2021A-007"]}]},{"name":"the Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University","award":["21JR7RA138"],"award-info":[{"award-number":["21JR7RA138"]}]},{"name":"the Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University","award":["NWNU-LKQN2019-21"],"award-info":[{"award-number":["NWNU-LKQN2019-21"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mineral nutrients, such as manganese (Mn) and iron (Fe), play essential roles in many biological processes in plants but their over-enrichment is harmful for the metabolism. Metal tolerance proteins (MTPs) are involved in cellular Mn and Fe homeostasis. However, the transporter responsible for the transport of Mn in wheat is unknown. In our study, TuMTP8, a Mn-CDF transporter from diploid wheat (Triticum urartu), was identified. Expression of TuMTP8 in yeast strains of \u0394ccc1 and \u0394smf1 and Arabidopsis conferred tolerance to elevated Mn and Fe, but not to other metals (zinc, cobalt, copper, nickel, or cadmium). Compared with TuVIT1 (vacuole Fe transporter), TuMTP8 shows a significantly higher proportion in Mn transport and a smaller proportion in Fe transport. The transient analysis in tobacco epidermal cells revealed that TuMTP8 localizes to vacuolar membrane. The highest transcript levels of TuMTP8 were in the sheath of the oldest leaf and the awn, suggesting that TuMTP8 sequesters excess Mn into the vacuole in these organs, away from more sensitive tissues. These findings indicate that TuMTP8, a tonoplast-localized Mn\/Fe transporter, functions as a primary balancer to regulate Mn distribution in T. urartu under elevated Mn conditions and participates in the intracellular transport and storage of excess Mn as a detoxification mechanism, thereby conferring Mn tolerance.<\/jats:p>","DOI":"10.3390\/ijms23105683","type":"journal-article","created":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T12:25:57Z","timestamp":1652963157000},"page":"5683","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["MTP8 from Triticum urartu Is Primarily Responsible for Manganese Tolerance"],"prefix":"10.3390","volume":"23","author":[{"given":"Fanhong","family":"Wang","sequence":"first","affiliation":[{"name":"College of Life Sciences, Northwest Normal University, Lanzhou 730070, China"},{"name":"College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Qiao","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanhuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2754-7052","authenticated-orcid":false,"given":"Hong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0345-0536","authenticated-orcid":false,"given":"Tuanyao","family":"Chai","sequence":"additional","affiliation":[{"name":"College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,19]]},"reference":[{"key":"ref_1","unstructured":"Marschner, H. 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