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In these cancers, tumor-derived extracellular vesicles (EVs) cause immunosuppression, facilitate the direct attachment and invasion of cancer cells through the mesothelium, induce the conversion of peritoneal mesothelial cells (PMCs) into cancer-associated fibroblasts (CAFs) and transfer a more aggressive phenotype amongst cancer cells. Although the promoting role of EVs in CRC and OvC peritoneal metastasis is well established, the specific molecules that mediate the interactions between tumor-derived EVs and immune and non-immune target cells remain elusive. Here, we employed the SKOV-3 (ovarian adenocarcinoma) and Colo-320 (colorectal adenocarcinoma) human cell lines as model systems to study the interactions and uptake of EVs produced by ovarian carcinoma and colorectal carcinoma cells, respectively. We established that the adhesion molecule ALCAM\/CD166 is involved in the interaction of cancer-derived EVs with recipient cancer cells (a process termed \u201cEV binding\u201d or \u201cEV docking\u201d) and in their subsequent uptake by these cells. The identification of ALCAM\/CD166 as a molecule mediating the docking and uptake of CRC and OvC-derived EVs may be potentially exploited to block the peritoneal metastasis cascade promoted by EVs in CRC and OvC patients.<\/jats:p>","DOI":"10.3390\/ijms23105753","type":"journal-article","created":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T13:56:12Z","timestamp":1653054972000},"page":"5753","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["ALCAM\/CD166 Is Involved in the Binding and Uptake of Cancer-Derived Extracellular Vesicles"],"prefix":"10.3390","volume":"23","author":[{"given":"Beatriz","family":"Carde\u00f1es","sequence":"first","affiliation":[{"name":"Centre for Molecular Biology \u201cSevero Ochoa\u201d (CSIC-UAM), Cell-Cell Communication & Inflammation Unit, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9804-8050","authenticated-orcid":false,"given":"Irene","family":"Clares","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology \u201cSevero Ochoa\u201d (CSIC-UAM), Cell-Cell Communication & Inflammation Unit, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0261-591X","authenticated-orcid":false,"given":"Tamara","family":"Bezos","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology \u201cSevero Ochoa\u201d (CSIC-UAM), Cell-Cell Communication & Inflammation Unit, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6573-6164","authenticated-orcid":false,"given":"V\u00edctor","family":"Toribio","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology \u201cSevero Ochoa\u201d (CSIC-UAM), Cell-Cell Communication & Inflammation Unit, 28049 Madrid, Spain"},{"name":"Department of Molecular Biology, Faculty of Sciences, Instituto Universitario de Biolog\u00eda Molecular, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soraya","family":"L\u00f3pez-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology \u201cSevero Ochoa\u201d (CSIC-UAM), Cell-Cell Communication & Inflammation Unit, 28049 Madrid, Spain"},{"name":"Department of Molecular Biology, Faculty of Sciences, Instituto Universitario de Biolog\u00eda Molecular, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9866-6035","authenticated-orcid":false,"given":"Almudena","family":"Rocha","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology \u201cSevero Ochoa\u201d (CSIC-UAM), Cell-Cell Communication & Inflammation Unit, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4256-3413","authenticated-orcid":false,"given":"H\u00e9ctor","family":"Peinado","sequence":"additional","affiliation":[{"name":"Microenvironment and Metastasis Laboratory, Molecular Oncology Programme, Spanish National Cancer Research Center (CNIO), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7484-2866","authenticated-orcid":false,"given":"Mar\u00eda","family":"Y\u00e1\u00f1ez-M\u00f3","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology \u201cSevero Ochoa\u201d (CSIC-UAM), Cell-Cell Communication & Inflammation Unit, 28049 Madrid, Spain"},{"name":"Department of Molecular Biology, Faculty of Sciences, Instituto Universitario de Biolog\u00eda Molecular, Universidad Aut\u00f3noma de Madrid, 28049 Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Hospital La Princesa, 28006 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8767-060X","authenticated-orcid":false,"given":"Carlos","family":"Caba\u00f1as","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology \u201cSevero Ochoa\u201d (CSIC-UAM), Cell-Cell Communication & Inflammation Unit, 28049 Madrid, Spain"},{"name":"Department of Immunology, Ophthalmology and Otorhinolaryngology, School of Medicine, Universidad Complutense de Madrid, 28040 Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Hospital 12 de Octubre (i+12), 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27066","DOI":"10.3402\/jev.v4.27066","article-title":"Biological Properties of Extracellular Vesicles and Their Physiological Functions","volume":"4","author":"Siljander","year":"2015","journal-title":"J. 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