{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T02:55:45Z","timestamp":1762052145098,"version":"build-2065373602"},"reference-count":94,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,5,21]],"date-time":"2022-05-21T00:00:00Z","timestamp":1653091200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian Government Research Training Program (RTP)","award":["APP1100737","LE180100202","LE200200045","DP170102020"],"award-info":[{"award-number":["APP1100737","LE180100202","LE200200045","DP170102020"]}]},{"name":"RTP Fee-Offset Scholarship","award":["APP1100737","LE180100202","LE200200045","DP170102020"],"award-info":[{"award-number":["APP1100737","LE180100202","LE200200045","DP170102020"]}]},{"DOI":"10.13039\/501100000925","name":"National Health &amp; Medical Research Council of Australia","doi-asserted-by":"publisher","award":["APP1100737","LE180100202","LE200200045","DP170102020"],"award-info":[{"award-number":["APP1100737","LE180100202","LE200200045","DP170102020"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["APP1100737","LE180100202","LE200200045","DP170102020"],"award-info":[{"award-number":["APP1100737","LE180100202","LE200200045","DP170102020"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Over 50% of the world\u2019s population is infected with Human Cytomegalovirus (HCMV). HCMV is responsible for serious complications in the immuno-compromised and is a leading cause of congenital birth defects. The molecular function of many HCMV proteins remains unknown, and a deeper understanding of the viral effectors that modulate virion maturation is required. In this study, we observed that UL34 is a viral protein expressed with leaky late kinetics that localises to the nucleus during infection. Deletion of UL34 from the HCMV genome (\u0394UL34) did not abolish the spread of HCMV. Instead, over &gt;100-fold fewer infectious virions were produced, so we report that UL34 is an augmenting gene. We found that \u0394UL34 is dispensable for viral DNA replication, and its absence did not alter the expression of IE1, MCP, gB, UL26, UL83, or UL99 proteins. In addition, \u0394UL34 infections were able to progress through the replication cycle to form a viral assembly compartment; however, virion maturation in the cytoplasm was abrogated. Further examination of the nucleus in \u0394UL34 infections revealed replication compartments with aberrant morphology, containing significantly less assembled capsids, with almost none undergoing subsequent maturation. Therefore, this work lays the foundation for UL34 to be further investigated in the context of nuclear organization and capsid maturation during HCMV infection.<\/jats:p>","DOI":"10.3390\/ijms23105773","type":"journal-article","created":{"date-parts":[[2022,5,21]],"date-time":"2022-05-21T09:18:08Z","timestamp":1653124688000},"page":"5773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["UL34 Deletion Restricts Human Cytomegalovirus Capsid Formation and Maturation"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7643-2796","authenticated-orcid":false,"given":"Declan L.","family":"Turner","sequence":"first","affiliation":[{"name":"Infection and Immunity Program, Department of Microbiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2800-5826","authenticated-orcid":false,"given":"Rachel M.","family":"Templin","sequence":"additional","affiliation":[{"name":"Ramaciotti Centre for Cryo Electron Microscopy, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adele A.","family":"Barugahare","sequence":"additional","affiliation":[{"name":"Infection and Immunity Program, Department of Microbiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia"},{"name":"Monash Bioinformatics Platform, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendan E.","family":"Russ","sequence":"additional","affiliation":[{"name":"Infection and Immunity Program, Department of Microbiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1002-0000","authenticated-orcid":false,"given":"Stephen J.","family":"Turner","sequence":"additional","affiliation":[{"name":"Infection and Immunity Program, Department of Microbiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georg","family":"Ramm","sequence":"additional","affiliation":[{"name":"Ramaciotti Centre for Cryo Electron Microscopy, Monash University, Clayton, VIC 3800, Australia"},{"name":"Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3064-4897","authenticated-orcid":false,"given":"Rommel A.","family":"Mathias","sequence":"additional","affiliation":[{"name":"Infection and Immunity Program, Department of Microbiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia"},{"name":"Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1002\/rmv.655","article-title":"Review of cytomegalovirus seroprevalence and demographic characteristics associated with infection","volume":"20","author":"Cannon","year":"2010","journal-title":"Rev. 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