{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T18:42:53Z","timestamp":1780598573856,"version":"3.54.1"},"reference-count":47,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T00:00:00Z","timestamp":1653264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health","award":["10\/2022"],"award-info":[{"award-number":["10\/2022"]}]},{"name":"M.I.Cro. Italia, Associazione Malattie Infiammatorie Croniche Intestinali","award":["10\/2022"],"award-info":[{"award-number":["10\/2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Defects in the intestinal epithelial barrier functions characterize inflammatory conditions such as Inflammatory Bowel Disease (IBD). Overexpression of pro-inflammatory cytokines such as TNF-\u03b1, IL-1B, IL-6 and INF-\u03b3 trigger epithelial damage. These cytokines are due to upregulation of claudin-2 (CLDN2) that form a pore channel, resulting in redistribution of TJs and an alteration of barrier permeability. Recently, we demonstrated that miR-195-5p is able to regulate CLDN2 and indirectly also CLDN1 in intestinal epithelial cells. Now, we aimed to investigate the modulation of miR-195-5p on the expression of CLDN2 and other TJs under inflammatory conditions induced by TNF-\u03b1. We demonstrated that miR-195-5p also modulated the expression of CLDN2 levels after stimulation with TNF-\u03b1. In addition, we discovered the role of miR-195-5p in the integrity of the intestinal barrier and in promoting the restoration of the intestinal epithelial. Moreover, we established that replacement of miR-195-5p attenuated the colonic inflammatory response in DSS-induced, colitis and it reduced colonic permeability. In conclusion, our data revealed the role of miR-195-5p in intestinal inflammation in ulcerative colitis, suggesting a potential pharmacological target for new therapeutic approaches.<\/jats:p>","DOI":"10.3390\/ijms23105840","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T22:57:22Z","timestamp":1653346642000},"page":"5840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["The Increase of miR-195-5p Reduces Intestinal Permeability in Ulcerative Colitis, Modulating Tight Junctions\u2019 Expression"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0273-2825","authenticated-orcid":false,"given":"Viviana","family":"Scalavino","sequence":"first","affiliation":[{"name":"National Institute of Gastroenterology \u201cS. de Bellis\u201d, IRCCS Research Hospital, 70013 Castellana Grotte, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7988-1917","authenticated-orcid":false,"given":"Emanuele","family":"Piccinno","sequence":"additional","affiliation":[{"name":"National Institute of Gastroenterology \u201cS. de Bellis\u201d, IRCCS Research Hospital, 70013 Castellana Grotte, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giusy","family":"Bianco","sequence":"additional","affiliation":[{"name":"National Institute of Gastroenterology \u201cS. de Bellis\u201d, IRCCS Research Hospital, 70013 Castellana Grotte, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00f2","family":"Schena","sequence":"additional","affiliation":[{"name":"National Institute of Gastroenterology \u201cS. de Bellis\u201d, IRCCS Research Hospital, 70013 Castellana Grotte, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1661-5504","authenticated-orcid":false,"given":"Raffaele","family":"Armentano","sequence":"additional","affiliation":[{"name":"National Institute of Gastroenterology \u201cS. de Bellis\u201d, IRCCS Research Hospital, 70013 Castellana Grotte, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluigi","family":"Giannelli","sequence":"additional","affiliation":[{"name":"National Institute of Gastroenterology \u201cS. de Bellis\u201d, IRCCS Research Hospital, 70013 Castellana Grotte, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2971-0802","authenticated-orcid":false,"given":"Grazia","family":"Serino","sequence":"additional","affiliation":[{"name":"National Institute of Gastroenterology \u201cS. de Bellis\u201d, IRCCS Research Hospital, 70013 Castellana Grotte, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/nri2653","article-title":"Intestinal mucosal barrier function in health and disease","volume":"9","author":"Turner","year":"2009","journal-title":"Nat. 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