{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:35:12Z","timestamp":1791588912638,"version":"4.3.4"},"reference-count":51,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T00:00:00Z","timestamp":1653350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2018YFD09011205"],"award-info":[{"award-number":["2018YFD09011205"]}]},{"name":"National Key R&amp;D Program of China","award":["32000384"],"award-info":[{"award-number":["32000384"]}]},{"name":"National Key R&amp;D Program of China","award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}]},{"name":"National Natural Science Foundation of China","award":["2018YFD09011205"],"award-info":[{"award-number":["2018YFD09011205"]}]},{"name":"National Natural Science Foundation of China","award":["32000384"],"award-info":[{"award-number":["32000384"]}]},{"name":"National Natural Science Foundation of China","award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["2018YFD09011205"],"award-info":[{"award-number":["2018YFD09011205"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["32000384"],"award-info":[{"award-number":["32000384"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Germline stem cells (GSCs) are a group of unique adult stem cells in gonads that act as important transmitters for genetic information. Donor GSCs have been used to produce offspring by transplantation in fisheries. In this study, we successfully isolated and enriched GSCs from the ovary, ovotestis, and testis of Monopterus albus, one of the most important breeding freshwater fishes in China. Transcriptome comparison assay suggests that a distinct molecular signature exists in each type of GSC, and that different signaling activities are required for the maintenance of distinct GSCs. Functional analysis shows that fGSCs can successfully colonize and contribute to the germline cell lineage of a host zebrafish gonad after transplantation. Finally, we describe a simple feeder-free method for the isolation and enrichment of GSCs that can contribute to the germline cell lineage of zebrafish embryos and generate the germline chimeras after transplantation.<\/jats:p>","DOI":"10.3390\/ijms23115861","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T03:16:55Z","timestamp":1653362215000},"page":"5861","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus"],"prefix":"10.3390","volume":"23","author":[{"given":"Xiaoyun","family":"Sun","sequence":"first","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8187-7052","authenticated-orcid":false,"given":"Binbin","family":"Tao","sequence":"additional","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Hu","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7902-9022","authenticated-orcid":false,"given":"Yuhua","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0044-8486(93)90286-8","article-title":"Hormonal Induction of Precocious Sex Reversal in the Ricefield Eel, Monopterus-Albus","volume":"118","author":"Tao","year":"1993","journal-title":"Aquaculture"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e8415","DOI":"10.7717\/peerj.8415","article-title":"Population genetics of swamp eel in the Yangtze River: Comparative analyses between mitochondrial and microsatellite data provide novel insights","volume":"8","author":"Zhou","year":"2020","journal-title":"PeerJ"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Chi, W., Gao, Y., Hu, Q., Guo, W., and Li, D.P. 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