{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:17:54Z","timestamp":1791231474493,"version":"4.1.0"},"reference-count":56,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T00:00:00Z","timestamp":1653523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>A retrospective case control study was undertaken at the molecular biology department of a private center for reproductive medicine in order to determine whether any correlation exists between the mitochondrial DNA (mtDNA) content of trophectoderm and embryo developmental potential. A total of 275 couples underwent IVF treatment, producing a total of 716 embryos. The trophectoderm was biopsied from each embryo at the blastocyst stage (day 5 or day 6 post-fertilization) subjected to low-pass next-generation sequencing (NGS), for the purpose of detecting aneuploidy. For each sample, the number of mtDNA reads obtained after analysis using NGS was divided by the number of reads attributable to the nuclear genome. The mtDNA copy number was found to be higher in aneuploid embryos than in those that were euploid (mean mtDNA ratio \u00b1 SD: 1.13 \u00b1 1.37 versus 1.45 \u00b1 1.78, p = 0.02) and in day 5 biopsies compared to day 6 biopsies (1.41 \u00b1 1.66 vs. 1.19 \u00b1 1.27, p = 0.001), whereas no statistically significant differences in mtDNA content were seen in relation to embryo morphology (1.58 \u00b1 2.44 vs. 2.19 \u00b1 2.89, p = 0.12), genetic sex (1.27 \u00b1 1.29 vs. 1.27 \u00b1 1.18, p = 0.99), maternal age (1.31 \u00b1 1.41 vs. 1.33 \u00b1 1.29, p = 0.43), or its ability to implant (1.14 \u00b1 0.88 vs. 1.21 \u00b1 1.16, p = 0.39). mtDNA has small potential to serve as an additional, independent biomarker for embryo selection.<\/jats:p>","DOI":"10.3390\/ijms23115976","type":"journal-article","created":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T08:50:22Z","timestamp":1653555022000},"page":"5976","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Does Trophectoderm Mitochondrial DNA Content Affect Embryo Developmental and Implantation Potential?"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5996-6991","authenticated-orcid":false,"given":"Krzysztof","family":"Lukaszuk","sequence":"first","affiliation":[{"name":"Invicta Research and Development Center, 81-740 Sopot, Poland"},{"name":"Department of Obstetrics and Gynecological Nursing, Faculty of Health Sciences, Medical University of Gdansk, 80-210 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Podolak","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecological Nursing, Faculty of Health Sciences, Medical University of Gdansk, 80-210 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1093\/humrep\/dez054","article-title":"Maternal Ageing Impairs Mitochondrial DNA Kinetics during Early Embryogenesis in Mice","volume":"34","author":"Brochard","year":"2019","journal-title":"Hum. 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