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These nascent tachyzoites bud off their mother and repeat the division process until the expanding progenies escape to settle and multiply in other host cells. Over these intra- and extra-cellular phases, the tachyzoite maintains an essential apicobasal polarity that emerges through a unique bidirectional budding process of the elongating cells. This process requires the assembly of several molecular complexes that, at the nascent pole, encompass structural and myosin motor elements. To characterize a recently identified basal pole marker named BCC7 with respect to the posterior myosin J and myosin C motors, we used conventional biochemistry as well as advanced proteomic and in silico analysis in conjunction with live and super resolution microscopy of transgenic fluorescent tachyzoites. We document that BCC7 forms a ribbed ring below which myosin C motor entities distribute regularly. In addition, we identified\u2014among 13 BCC7 putative partners\u2014two novel and five known members of the inner membrane complex (IMC) family which ends at the apical side of the ring. Therefore, BCC7 could assist the stabilization of the IMC plaques and contribute to the parasite biomechanical properties.<\/jats:p>","DOI":"10.3390\/ijms23115995","type":"journal-article","created":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T08:50:22Z","timestamp":1653555022000},"page":"5995","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The BCC7 Protein Contributes to the Toxoplasma Basal Pole by Interfacing between the MyoC Motor and the IMC Membrane Network"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9733-2770","authenticated-orcid":false,"given":"Luis","family":"Vigetti","sequence":"first","affiliation":[{"name":"IAB, Team Biomechanics of Host-Apicomplexa Parasite, INSERM U1209, CNRS UMR5309, Grenoble Alpes University, 38700 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Labour\u00e9","sequence":"additional","affiliation":[{"name":"IAB, Team Biomechanics of Host-Apicomplexa Parasite, INSERM U1209, CNRS UMR5309, Grenoble Alpes University, 38700 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"Roum\u00e9gous","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bordeaux, Team Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, CNRS UMR 5234, 33000 Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Cannella","sequence":"additional","affiliation":[{"name":"IAB, Team Host-Pathogen Interactions & Immunity to Infection, INSERM U1209, CNRS UMR5309, Grenoble Alpes University, 38700 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bastien","family":"Touquet","sequence":"additional","affiliation":[{"name":"IAB, Team Biomechanics of Host-Apicomplexa Parasite, INSERM U1209, CNRS UMR5309, Grenoble Alpes University, 38700 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudine","family":"Mayer","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, 75013 Paris, France"},{"name":"ICube-UMR7357, CSTB, Centre de Recherche en Biom\u00e9decine de Strasbourg, 67084 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3896-6196","authenticated-orcid":false,"given":"Yohann","family":"Cout\u00e9","sequence":"additional","affiliation":[{"name":"INSERM, University of Grenoble Alpes, CEA, UMR BioSant\u00e9 U1292, CNRS, CEA, FR2048, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8952-2598","authenticated-orcid":false,"given":"Karine","family":"Fr\u00e9nal","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bordeaux, Team Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, CNRS UMR 5234, 33000 Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Tardieux","sequence":"additional","affiliation":[{"name":"IAB, Team Biomechanics of Host-Apicomplexa Parasite, INSERM U1209, CNRS UMR5309, Grenoble Alpes University, 38700 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Renesto","sequence":"additional","affiliation":[{"name":"IAB, Team Biomechanics of Host-Apicomplexa Parasite, INSERM U1209, CNRS UMR5309, Grenoble Alpes University, 38700 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"656","DOI":"10.3201\/eid1504.081306","article-title":"Severe Acquired Toxoplasmosis Caused by Wild Cycle of Toxoplasma gondii, French Guiana","volume":"15","author":"Carme","year":"2009","journal-title":"Emerg. 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