{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T09:11:44Z","timestamp":1771492304818,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamental Research Funds for the Central Universities","award":["2021ZY84"],"award-info":[{"award-number":["2021ZY84"]}]},{"name":"Fundamental Research Funds for the Central Universities","award":["31971770"],"award-info":[{"award-number":["31971770"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2021ZY84"],"award-info":[{"award-number":["2021ZY84"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31971770"],"award-info":[{"award-number":["31971770"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The NYC-like (NOL) enzyme is considered as an essential enzyme for chlorophyll b degradation, which catalyzes the formation of 7-hydroxymethyl chlorophyll a from chlorophyll b. The ZjNOL gene was cloned from Zoysia japonica with a completed coding sequence of 981-bp in length, encoding 326 amino acids. ZjNOL was localized on the stroma side of the thylakoid membrane, and co-localized with ZjNYC in the chloroplasts. Multiple photoregulatory elements and hormone regulatory elements were identified in the promoter region of the ZjNOL gene, and the expression level of the ZjNOL gene was dramatically up-regulated in senescence leaves, which were regulated by a variety of plant hormones. ZjNOL\u2019s ectopic expression in creeping bentgrass produced yellow leaves, thicker cortex, and smaller vascular column cells. Additionally, transgenic plants exhibited morphological alterations in their chloroplast structure, and the number of grana and thylakoids per grana stack reduced dramatically. Transgenic plants also had a lower photosynthetic rate and Fm\/Fv than the control. The transgenic plants displayed a decreased chlorophyll content and a greater rate of ion leakage. The properties and activities of ZjNOL will serve as a foundation for future research into gene functions and regulatory processes.<\/jats:p>","DOI":"10.3390\/ijms23116032","type":"journal-article","created":{"date-parts":[[2022,5,28]],"date-time":"2022-05-28T01:40:45Z","timestamp":1653702045000},"page":"6032","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Expression of a Chlorophyll b Reductase Gene from Zoysia japonica Causes Changes in Leaf Color and Chlorophyll Morphology in Agrostis stolonifera"],"prefix":"10.3390","volume":"23","author":[{"given":"Di","family":"Dong","sequence":"first","affiliation":[{"name":"School of Grassland Science, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuoxiong","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Grassland Science, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Grassland Science, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuwen","family":"Li","sequence":"additional","affiliation":[{"name":"School of Grassland Science, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengdi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Grassland Science, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinruizhi","family":"Li","sequence":"additional","affiliation":[{"name":"School of Grassland Science, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5376-4878","authenticated-orcid":false,"given":"Zhuocheng","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Grassland Science, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liebao","family":"Han","sequence":"additional","affiliation":[{"name":"School of Grassland Science, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0591-1092","authenticated-orcid":false,"given":"Yuehui","family":"Chao","sequence":"additional","affiliation":[{"name":"School of Grassland Science, Beijing Forestry University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1007\/s00425-004-1231-8","article-title":"The Loss of Green Color during Chlorophyll Degradation\u2014A Prerequisite to Prevent Cell Death?","volume":"219","year":"2004","journal-title":"Planta"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/S0959-440X(94)90214-3","article-title":"Structure and Function of the Plant Light-Harvesting Complex, LHC-II","volume":"4","author":"KiJhlbrandt","year":"1994","journal-title":"Curr. 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