{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T16:09:44Z","timestamp":1782058184499,"version":"3.54.5"},"reference-count":36,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Increasing the Performance of Scientific Research, Supporting Excellence in Medical Research and Innovation, PROGRES","award":["40PFE\/20.12.2021"],"award-info":[{"award-number":["40PFE\/20.12.2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Background: Cervical cancer is one of the most common malignancies in women in terms of prevalence and mortality. Cervical cancer has some particularities that distinguish it from any other oncologic pathology: first, it is completely preventable by prompt detection of its precursor, cervical intraepithelial neoplasia (CIN); second, the Human Papillomavirus (HPV) infection is a known etiological agent; third, the mean age at diagnosis is much lower than in other oncologic conditions, as a consequence of the sexually-transmitted HPV. Methods: We evaluated the expression level of several long noncoding RNAs and a microRNA in samples from 30 patients with CIN, 9 with cervical cancer and 38 normal samples using qRT-PCR technology. Results: We observed higher expression levels for MEG3, DAPK1, MLH1 and MALAT1 in CIN samples than in normal samples, whereas TIMP3 and SOX1 had lower expression levels. For cancer samples, DAPK1, MLH1 and MALAT1 had higher expression, and MEG3, TIMP3 and SOX1 had lower expression when compared to normal samples. In the case of CIN versus cancer samples, only MEG3 gene showed a statistically significant difference. The expression of miR-205-5p was lower in both CIN and cancer samples compared to normal samples. Conclusion: Decreased MEG3 expression could be considered an alarm signal in the transition from a premalignant cervical lesion to invasive cancer, while altered expression levels of TIMP3, SOX1, MLH1, MALAT1 and miR-205-5p could serve as early biomarkers in the diagnosis of premalignant cervical lesions. Future studies, including a larger number of patients with CIN, will be of particular importance in validating these observations.<\/jats:p>","DOI":"10.3390\/ijms23116054","type":"journal-article","created":{"date-parts":[[2022,5,28]],"date-time":"2022-05-28T01:40:45Z","timestamp":1653702045000},"page":"6054","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Alteration of Gene and miRNA Expression in Cervical Intraepithelial Neoplasia and Cervical Cancer"],"prefix":"10.3390","volume":"23","author":[{"given":"Marina","family":"Dudea-Simon","sequence":"first","affiliation":[{"name":"2nd Obstetrics and Gynecology Department, \u201cDominic Stanca\u201d Obstetrics and Gynecology Clinic, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Mihu","sequence":"additional","affiliation":[{"name":"2nd Obstetrics and Gynecology Department, \u201cDominic Stanca\u201d Obstetrics and Gynecology Clinic, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5806-0220","authenticated-orcid":false,"given":"Laura Ancuta","family":"Pop","sequence":"additional","affiliation":[{"name":"Research Center for Functional Genomics, Biomedicine and Translational Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 15 Victor Babe\u0219 Street, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Razvan","family":"Ciortea","sequence":"additional","affiliation":[{"name":"2nd Obstetrics and Gynecology Department, \u201cDominic Stanca\u201d Obstetrics and Gynecology Clinic, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei Mihai","family":"Malutan","sequence":"additional","affiliation":[{"name":"2nd Obstetrics and Gynecology Department, \u201cDominic Stanca\u201d Obstetrics and Gynecology Clinic, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doru","family":"Diculescu","sequence":"additional","affiliation":[{"name":"2nd Obstetrics and Gynecology Department, \u201cDominic Stanca\u201d Obstetrics and Gynecology Clinic, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0625-4445","authenticated-orcid":false,"given":"Cristina Alexandra","family":"Ciocan","sequence":"additional","affiliation":[{"name":"Research Center for Functional Genomics, Biomedicine and Translational Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 15 Victor Babe\u0219 Street, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7450-9454","authenticated-orcid":false,"given":"Roxana Maria","family":"Cojocneanu","sequence":"additional","affiliation":[{"name":"Research Center for Functional Genomics, Biomedicine and Translational Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 15 Victor Babe\u0219 Street, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasile","family":"Simon","sequence":"additional","affiliation":[{"name":"Urology Department, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Bucuri","sequence":"additional","affiliation":[{"name":"2nd Obstetrics and Gynecology Department, \u201cDominic Stanca\u201d Obstetrics and Gynecology Clinic, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radu","family":"Mocan-Hognogi","sequence":"additional","affiliation":[{"name":"2nd Obstetrics and Gynecology Department, \u201cDominic Stanca\u201d Obstetrics and Gynecology Clinic, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3055-4747","authenticated-orcid":false,"given":"Cornelia","family":"Braicu","sequence":"additional","affiliation":[{"name":"Research Center for Functional Genomics, Biomedicine and Translational Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 15 Victor Babe\u0219 Street, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5828-1325","authenticated-orcid":false,"given":"Ioana","family":"Berindan-Neagoe","sequence":"additional","affiliation":[{"name":"Research Center for Functional Genomics, Biomedicine and Translational Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 15 Victor Babe\u0219 Street, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,27]]},"reference":[{"key":"ref_1","unstructured":"Ferlay, J., Ervik, M., Lam, F., Colombet, M., Mery, L., Pi\u00f1eros, M., Znaor, A., Soerjomataram, I., and Bray, F. 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