{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T22:53:05Z","timestamp":1773442385247,"version":"3.50.1"},"reference-count":86,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,5,28]],"date-time":"2022-05-28T00:00:00Z","timestamp":1653696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Agriculture and Rural Affairs","award":["CARS-28-16"],"award-info":[{"award-number":["CARS-28-16"]}]},{"name":"Ministry of Agriculture and Rural Affairs","award":["2019YFD1001800"],"award-info":[{"award-number":["2019YFD1001800"]}]},{"name":"Ministry of Science and Technology of China","award":["CARS-28-16"],"award-info":[{"award-number":["CARS-28-16"]}]},{"name":"Ministry of Science and Technology of China","award":["2019YFD1001800"],"award-info":[{"award-number":["2019YFD1001800"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Botryosphaeria spp. are important phytopathogenic fungi that infect a wide range of woody plants, resulting in big losses worldwide each year. However, their pathogenetic mechanisms and the related virulence factors are rarely addressed. In this study, seven lignin peroxidase (LiP) paralogs were detected in Botryosphaeria kuwatsukai, named BkLiP1 to BkLiP7, respectively, while only BkLiP1 was identified as responsible for the vegetative growth and virulence of B. kuwatsukai as assessed in combination with knock-out, complementation, and overexpression approaches. Moreover, BkLiP1, with the aid of a signal peptide (SP), is translocated onto the cell wall of B. kuwatsukai and secreted into the apoplast space of plant cells as expressed in the leaves of Nicotiana benthamiana, which can behave as a microbe-associated molecular pattern (MAMP) to trigger the defense response of plants, including cell death, reactive oxygen species (ROS) burst, callose deposition, and immunity-related genes up-regulated. It supports the conclusion that BkLiP1 plays an important role in the virulence and vegetative growth of B. kuwatsukai and alternatively behaves as an MAMP to induce plant cell death used for the fungal version, which contributes to a better understanding of the pathogenetic mechanism of Botryosphaeria fungi.<\/jats:p>","DOI":"10.3390\/ijms23116066","type":"journal-article","created":{"date-parts":[[2022,5,29]],"date-time":"2022-05-29T08:50:51Z","timestamp":1653814251000},"page":"6066","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response"],"prefix":"10.3390","volume":"23","author":[{"given":"Feng","family":"Xiao","sequence":"first","affiliation":[{"name":"College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Plant Pathology of Hubei Province, Wuhan 430070, China"},{"name":"Key Laboratory of Horticultural Crop (Fruit Trees) Biology, and Germplasm Creation of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxing","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Plant Pathology of Hubei Province, Wuhan 430070, China"},{"name":"Key Laboratory of Horticultural Crop (Fruit Trees) Biology, and Germplasm Creation of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Hubei Hongshan Laboratory, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ni","family":"Hong","sequence":"additional","affiliation":[{"name":"College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Plant Pathology of Hubei Province, Wuhan 430070, China"},{"name":"Key Laboratory of Horticultural Crop (Fruit Trees) Biology, and Germplasm Creation of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9095-8682","authenticated-orcid":false,"given":"Liping","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Plant Pathology of Hubei Province, Wuhan 430070, China"},{"name":"Key Laboratory of Horticultural Crop (Fruit Trees) Biology, and Germplasm Creation of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongle","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Plant Pathology of Hubei Province, Wuhan 430070, China"},{"name":"Key Laboratory of Horticultural Crop (Fruit Trees) Biology, and Germplasm Creation of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoping","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Plant Pathology of Hubei Province, Wuhan 430070, China"},{"name":"Key Laboratory of Horticultural Crop (Fruit Trees) Biology, and Germplasm Creation of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1111\/mpp.12495","article-title":"Botryosphaeria dothidea: A latent pathogen of global importance to woody plant health","volume":"18","author":"Marsberg","year":"2017","journal-title":"Mol. 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