{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T13:57:40Z","timestamp":1769090260465,"version":"3.49.0"},"reference-count":41,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T00:00:00Z","timestamp":1654128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The tyrosinase enzyme, which catalyzes the hydroxylation of monophenols and the oxidation of o-diphenols, is typically involved in the synthesis of the dark product melanin starting from the amino acid tyrosine. Contributing to the browning of plant and fruit tissues and to the hyperpigmentation of the skin, leading to melasma or age spots, the research of possible tyrosinase inhibitors has attracted much interest in agri-food, cosmetic, and medicinal industries. In this study, we analyzed the capability of antamanide, a mushroom bioactive cyclic decapeptide, and some of its glycine derivatives, compared to that of pseudostellarin A, a known tyrosinase inhibitor, to hinder tyrosinase activity by using a spectrophotometric method. Additionally, computational docking studies were performed in order to elucidate the interactions occurring with the tyrosinase catalytic site. Our results show that antamanide did not exert any inhibitory activity. On the contrary, the three glycine derivatives AG9, AG6, and AOG9, which differ from each other by the position of a glycine that substitutes phenylalanine in the parent molecule, improving water solubility and flexibility, showed tyrosinase inhibition by spectrophotometric assays. Analytical data were confirmed by computational studies.<\/jats:p>","DOI":"10.3390\/ijms23116240","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T11:05:02Z","timestamp":1654167902000},"page":"6240","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Antamanide Analogs as Potential Inhibitors of Tyrosinase"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9720-6176","authenticated-orcid":false,"given":"Claudia","family":"Honisch","sequence":"first","affiliation":[{"name":"Institute of Biomolecular Chemistry of CNR, Padova Unit, Via F. Marzolo, 1, 35131 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matteo","family":"Gazziero","sequence":"additional","affiliation":[{"name":"Institute of Biomolecular Chemistry of CNR, Padova Unit, Via F. Marzolo, 1, 35131 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4626-4792","authenticated-orcid":false,"given":"Roberto","family":"Dallocchio","sequence":"additional","affiliation":[{"name":"Institute of Biomolecular Chemistry of CNR, Sassari Unit, Traversa La Crucca, 3, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8258-7332","authenticated-orcid":false,"given":"Alessandro","family":"Dess\u00ec","sequence":"additional","affiliation":[{"name":"Institute of Biomolecular Chemistry of CNR, Sassari Unit, Traversa La Crucca, 3, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3947-5164","authenticated-orcid":false,"given":"Davide","family":"Fabbri","sequence":"additional","affiliation":[{"name":"Institute of Biomolecular Chemistry of CNR, Sassari Unit, Traversa La Crucca, 3, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8776-9271","authenticated-orcid":false,"given":"Maria Antonietta","family":"Dettori","sequence":"additional","affiliation":[{"name":"Institute of Biomolecular Chemistry of CNR, Sassari Unit, Traversa La Crucca, 3, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Delogu","sequence":"additional","affiliation":[{"name":"Institute of Biomolecular Chemistry of CNR, Sassari Unit, Traversa La Crucca, 3, 07100 Sassari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5596-9295","authenticated-orcid":false,"given":"Paolo","family":"Ruzza","sequence":"additional","affiliation":[{"name":"Institute of Biomolecular Chemistry of CNR, Padova Unit, Via F. Marzolo, 1, 35131 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"196","DOI":"10.2174\/1389203719666180613090710","article-title":"Research Progress of Bioactive Proteins from the Edible and Medicinal Mushrooms","volume":"20","author":"Zhou","year":"2019","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Landi, N., Clemente, A., Pedone, P.V., Ragucci, S., and Di Maro, A. (2022). An Updated Review of Bioactive Peptides from Mushrooms in a Well-Defined Molecular Weight Range. Toxins, 14.","DOI":"10.3390\/toxins14020084"},{"key":"ref_3","unstructured":"Zechmeister, L. (1967). The Toxic Peptides of Amanita Phalloides. 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