{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T16:06:09Z","timestamp":1792253169880,"version":"4.4.0"},"reference-count":29,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T00:00:00Z","timestamp":1654300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic rhinosinusitis with nasal polyps (CRSwNP) is associated with inflammation and tissue remodeling including myofibroblasts differentiation and extracellular matrix (ECM) deposition mediated by TGF-\u03b21 and IL-4. Oncostatin M (OSM) is a cytokine involved in fibrotic processes in other cellular subtypes. We investigated the mechanisms of action of OSM in the fibrosis process associated with CRSwNP. The expression of IL-4, OSM and TGF-\u03b21 was assessed by RT-qPCR. Primary human cultures of nasal-polyp-derived fibroblasts were established and stimulated by TGF-\u03b21 and\/or IL-4 and\/or OSM. The expression of ECM components and \u03b1SMA was determined by RT-qPCR and Western blot. TGF-\u03b21-Smad3 signaling was investigated by immunofluorescence. TGF-\u03b21, IL-4 and OSM as well as \u03b1SMA were overexpressed in nasal polyps when compared to noninflammatory nasal mucosa. In TGF-\u03b21-stimulated nasal-polyp-derived fibroblasts, ECM genes and \u03b1SMA gene and protein were overexpressed, as well as \u03b1SMA in IL-4-stimulated fibroblasts. OSM counteracted the profibrotic effect of TGF-\u03b21 on ECM components and \u03b1SMA. TGF-\u03b21-induced nuclear translocation of Smad3 was completely reversed by OSM. OSM counteracts the profibrotic effect of IL-4 and also TGF-\u03b21, by inhibiting the nuclear translocation of Smad3. We suggest OSM could be an efficient tool to protect against fibrosis in CRSwNP.<\/jats:p>","DOI":"10.3390\/ijms23116308","type":"journal-article","created":{"date-parts":[[2022,6,5]],"date-time":"2022-06-05T10:47:11Z","timestamp":1654426031000},"page":"6308","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Oncostatin M Counteracts the Fibrotic Effects of TGF-\u03b21 and IL-4 on Nasal-Polyp-Derived Fibroblasts: A Control of Fibrosis in Chronic Rhinosinusitis with Nasal Polyps?"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5574-8559","authenticated-orcid":false,"given":"Florent","family":"Carsuzaa","sequence":"first","affiliation":[{"name":"Laboratoire Inflammation Tissus Epith\u00e9liaux et Cytokines (LITEC), UR15560, Universit\u00e9 de Poitiers, 86000 Poitiers, France"},{"name":"Service ORL, Chirurgie Cervico-Maxillo-Faciale et Audiophonologie, Centre Hospitalier Universitaire de Poitiers, 86000 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9milie","family":"B\u00e9quignon","sequence":"additional","affiliation":[{"name":"Service ORL et Chirurgie Cervico-Faciale, Centre Hospitalier Intercommunal de Cr\u00e9teil, 94000 Cr\u00e9teil, France"},{"name":"Laboratoire INSERM UMR955 Eq13, Institut Mondor de Recherche Biom\u00e9dicale, 94000 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Bainaud","sequence":"additional","affiliation":[{"name":"Laboratoire Inflammation Tissus Epith\u00e9liaux et Cytokines (LITEC), UR15560, Universit\u00e9 de Poitiers, 86000 Poitiers, France"},{"name":"Service Immunologie et Inflammation, Centre Hospitalier Universitaire de Poitiers, 86021 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9703-5976","authenticated-orcid":false,"given":"Jean-Fran\u00e7ois","family":"J\u00e9gou","sequence":"additional","affiliation":[{"name":"Laboratoire Inflammation Tissus Epith\u00e9liaux et Cytokines (LITEC), UR15560, Universit\u00e9 de Poitiers, 86000 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Dufour","sequence":"additional","affiliation":[{"name":"Laboratoire Inflammation Tissus Epith\u00e9liaux et Cytokines (LITEC), UR15560, Universit\u00e9 de Poitiers, 86000 Poitiers, France"},{"name":"Service ORL, Chirurgie Cervico-Maxillo-Faciale et Audiophonologie, Centre Hospitalier Universitaire de Poitiers, 86000 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Claude","family":"Lecron","sequence":"additional","affiliation":[{"name":"Laboratoire Inflammation Tissus Epith\u00e9liaux et Cytokines (LITEC), UR15560, Universit\u00e9 de Poitiers, 86000 Poitiers, France"},{"name":"Service Immunologie et Inflammation, Centre Hospitalier Universitaire de Poitiers, 86021 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Favot","sequence":"additional","affiliation":[{"name":"Laboratoire Inflammation Tissus Epith\u00e9liaux et Cytokines (LITEC), UR15560, Universit\u00e9 de Poitiers, 86000 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4193\/Rhin20.401","article-title":"European position paper on rhinosinusitis and nasal polyps 2020","volume":"58","author":"Fokkens","year":"2020","journal-title":"Rhinology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/00130832-200302000-00001","article-title":"Nasal polyposis: An update: Editorial review","volume":"3","author":"Pawankar","year":"2003","journal-title":"Curr. 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