{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:17:35Z","timestamp":1792804655930,"version":"4.5.0"},"reference-count":160,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T00:00:00Z","timestamp":1654560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Surgery, Oncology and Gastroenterology (DiSCOG), University of Padua","id":[{"id":"https:\/\/ror.org\/00240q980","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the case of pediatric urology there are several congenital conditions, such as hypospadias and neurogenic bladder, which affect, respectively, the urethra and the urinary bladder. In fact, the gold standard consists of a urethroplasty procedure in the case of urethral malformations and enterocystoplasty in the case of urinary bladder disorders. However, both surgical procedures are associated with severe complications, such as fistulas, urethral strictures, and dehiscence of the repair or recurrence of chordee in the case of urethroplasty, and metabolic disturbances, stone formation, urine leakage, and chronic infections in the case of enterocystoplasty. With the aim of overcoming the issue related to the lack of sufficient and appropriate autologous tissue, increasing attention has been focused on tissue engineering. In this review, both the urethral and the urinary bladder reconstruction strategies were summarized, focusing on pediatric applications and evaluating all the biomaterials tested in both animal models and patients. Particular attention was paid to the capability for tissue regeneration in dependence on the eventual presence of seeded cell and growth factor combinations in several types of scaffolds. Moreover, the main critical features needed for urinary tissue engineering have been highlighted and specifically focused on for pediatric application.<\/jats:p>","DOI":"10.3390\/ijms23126360","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T02:25:33Z","timestamp":1654827933000},"page":"6360","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Tissue Engineering and Regenerative Medicine in Pediatric Urology: Urethral and Urinary Bladder Reconstruction"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8212-1484","authenticated-orcid":false,"given":"Martina","family":"Casarin","sequence":"first","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology, Giustiniani 2, 35128 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Morlacco","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology, Giustiniani 2, 35128 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Dal Moro","sequence":"additional","affiliation":[{"name":"Department of Surgery, Oncology and Gastroenterology, Giustiniani 2, 35128 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3389\/fped.2019.00091","article-title":"Tissue engineering in pediatric bladder reconstruction-The road to success","volume":"7","author":"Horst","year":"2019","journal-title":"Front. Pediatr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"283","DOI":"10.3389\/fped.2017.00283","article-title":"Current status of tissue engineering in the management of severe hypospadias","volume":"5","author":"Abbas","year":"2018","journal-title":"Front. Pediatr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1038\/s41585-020-0281-4","article-title":"The current state of tissue engineering in the management of hypospadias","volume":"17","author":"Chan","year":"2020","journal-title":"Nat. Rev. Urol."},{"key":"ref_4","first-page":"107","article-title":"Tissue engineering in pediatric urology-A critical appraisal","volume":"3","author":"Stehr","year":"2020","journal-title":"Innov. Surg. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1097\/MOU.0b013e328302f0e1","article-title":"Current status of tissue engineering in pediatric urology","volume":"18","author":"Yamzon","year":"2008","journal-title":"Curr. Opin. Urol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Casarin, M., Morlacco, A., and Dal Moro, F. (2021). Bladder Substitution: The Role of Tissue Engineering and Biomaterials. Processes, 9.","DOI":"10.3390\/pr9091643"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Serrano-Aroca, \u00c1., Vera-Donoso, C.D., and Moreno-Manzano, V. (2018). Bioengineering approaches for bladder regeneration. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19061796"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s00345-008-0318-4","article-title":"Does mechanical stimulation have any role in urinary bladder tissue engineering?","volume":"26","author":"Farhat","year":"2008","journal-title":"World J. Urol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.sxmr.2017.08.004","article-title":"Urinary Tissue Engineering: Challenges and Opportunities","volume":"6","author":"Singh","year":"2018","journal-title":"Sex. Med. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7447","DOI":"10.1016\/j.biomaterials.2012.06.087","article-title":"In-vivo performance of high-density collagen gel tubes for urethral regeneration in a rabbit model","volume":"33","author":"Micol","year":"2012","journal-title":"Biomaterials"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1590\/S1677-5538.IBJU.2013.03.16","article-title":"Integration of collagen matrices into the urethra when implanted as onlay graft","volume":"39","author":"Sayeg","year":"2013","journal-title":"Int. Braz. J. Urol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.actbio.2016.07.033","article-title":"Engineered acellular collagen scaffold for endogenous cell guidance, a novel approach in urethral regeneration","volume":"43","author":"Pinnagoda","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1007\/s00345-016-1959-3","article-title":"Collagen cell carriers seeded with human urothelial cells for urethral reconstructive surgery: First results in a xenograft minipig model","volume":"35","author":"Aufderklamm","year":"2017","journal-title":"World J. Urol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1046\/j.1464-410X.1998.00828.x","article-title":"In vitro assessment of a collagen sponge for engineering urothelial grafts","volume":"82","author":"Sabbagh","year":"1998","journal-title":"Br. J. Urol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1089\/ten.tec.2009.0311","article-title":"Development of a porcine bladder acellular matrix with well-preserved extracellular bioactive factors for tissue engineering","volume":"16","author":"Yang","year":"2010","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.jss.2013.04.016","article-title":"Evaluation of stretched electrospun silk fibroin matrices seeded with urothelial cells for urethra reconstruction","volume":"184","author":"Xie","year":"2013","journal-title":"J. Surg. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.biomaterials.2015.02.092","article-title":"Acellular bi-layer silk fibroin scaffolds support functional tissue regeneration in a rat model of onlay esophagoplasty","volume":"53","author":"Algarrahi","year":"2015","journal-title":"Biomaterials"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3415","DOI":"10.1016\/j.biomaterials.2008.05.002","article-title":"In vivo degradation of 3D Silk Fibroin Scaffolds","volume":"29","author":"Wang","year":"2008","journal-title":"Biomaterials"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jss.2013.11.1102","article-title":"Tissue-engineered buccal mucosa using silk fibroin matrices for urethral reconstruction in a canine model","volume":"188","author":"Xie","year":"2014","journal-title":"J. Surg. Res."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Chung, Y.G., Tu, D., Franck, D., Gil, E.S., Algarrahi, K., Adam, R.M., Kaplan, D.L., Estrada, C.R., and Mauney, J.R. (2014). Acellular bi-layer silk fibroin scaffolds support tissue regeneration in a rabbit model of onlay urethroplasty. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0091592"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Casarin, M., Fortunato, T.M., Imran, S., Todesco, M., Sandrin, D., Borile, G., Toniolo, I., Marchesan, M., Gerosa, G., and Bagno, A. (2022). Porcine Small Intestinal Submucosa ( SIS ) as a Suitable Scaffold for the Creation of a Tissue-Engineered Urinary Conduit: Decellularization, Biomechanical and Biocompatibility Characterization Using New Approaches. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23052826"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1016\/j.transproceed.2007.02.092","article-title":"The Artificial Conduit for Urinary Diversion in Rats: A Preliminary Study","volume":"39","author":"Drewa","year":"2007","journal-title":"Transplant. Proc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1016\/S0022-5347(01)66117-2","article-title":"Regenerative urinary bladder augmentation using small intestinal submucosa: Urodynamic and histopathologic assessment in long-term canine bladder augmentations","volume":"155","author":"Kropp","year":"1996","journal-title":"J. Urol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1016\/j.biomaterials.2010.10.006","article-title":"Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering","volume":"32","author":"Wu","year":"2011","journal-title":"Biomaterials"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.eururo.2004.04.019","article-title":"Bladder cell culture on small intestinal submucosa as bioscaffold: Experimental study on engineered urothelial grafts","volume":"46","author":"Campodonico","year":"2004","journal-title":"Eur. Urol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1016\/j.biomaterials.2009.04.008","article-title":"Optimization of a natural collagen scaffold to aid cell-matrix penetration for urologic tissue engineering","volume":"30","author":"Liu","year":"2009","journal-title":"Biomaterials"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"335","DOI":"10.3760\/cma.j.issn.0366-6999.20120443","article-title":"Tissue-engineered conduit using bladder acellular matrix and bladder epithelial cells for urinary diversion in rabbits","volume":"126","author":"Liao","year":"2013","journal-title":"Chin. Med. J. (Engl.)"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1089\/ten.tea.2016.0518","article-title":"Transplantation of Amniotic Scaffold-Seeded Mesenchymal Stem Cells and\/or Endothelial Progenitor Cells From Bone Marrow to Efficiently Repair 3-cm Circumferential Urethral Defect in Model Dogs","volume":"24","author":"Chen","year":"2018","journal-title":"Tissue Eng. Part A"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1111\/j.1464-410X.2004.05047.x","article-title":"In vitro assessment of decellularized porcine dermis as a matrix for urinary tract reconstruction","volume":"94","author":"Kimuli","year":"2004","journal-title":"BJU Int."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/6146","article-title":"De novo reconstitution of a functional mammalian urinary bladder by tissue engineering","volume":"17","author":"Oberpenning","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1016\/S0022-5347(05)64429-1","article-title":"Phenotypic and functional characterization of in vivo tissue engineered smooth muscle from normal and pathological bladders","volume":"168","author":"Lai","year":"2002","journal-title":"J. Urol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1016\/j.jpedsurg.2003.08.034","article-title":"Tissue-Engineered Urinary Bladder Wall Using PLGA Mesh-Collagen Hybrid Scaffolds: A Comparison Study of Collagen Sponge and Gel as a Scaffold","volume":"38","author":"Nakanishi","year":"2003","journal-title":"J. Pediatr. Surg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/S0022-5347(17)35561-1","article-title":"Implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human bladder muscle","volume":"150","author":"Atala","year":"1993","journal-title":"J. Urol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/S0140-6736(06)68438-9","article-title":"Tissue-engineered autologous bladders for patients needing cystoplasty","volume":"367","author":"Atala","year":"2006","journal-title":"Lancet"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/term.2112","article-title":"The potential role of tissue-engineered urethral substitution: Clinical and preclinical studies","volume":"11","author":"Atala","year":"2017","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3969","DOI":"10.1016\/j.biomaterials.2011.02.012","article-title":"A collagen-poly(lactic acid-co-e{open}-caprolactone) hybrid scaffold for bladder tissue regeneration","volume":"32","author":"Engelhardt","year":"2011","journal-title":"Biomaterials"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5824","DOI":"10.1038\/s41598-020-62197-3","article-title":"Development of a conductive biocomposite combining graphene and amniotic membrane for replacement of the neuronal network of tissue-engineered urinary bladder","volume":"10","author":"Adamowicz","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Adamowicz, J., Pokrywczy\u0144ska, M., Tworkiewicz, J., Kowalczyk, T., Van Breda, S.V., Tyloch, D., Kloskowski, T., Bodnar, M., Skopinska-Wisniewska, J., and Marsza\u0142ek, A. (2016). New amniotic membrane based biocomposite for future application in reconstructive urology. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146012"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1016\/j.biomaterials.2012.10.075","article-title":"A bilayered hybrid microfibrous PLGA-Acellular matrix scaffold for hollow organ tissue engineering","volume":"34","author":"Horst","year":"2013","journal-title":"Biomaterials"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.ymeth.2008.10.014","article-title":"Composite scaffolds for the engineering of hollow organs and tissues","volume":"47","author":"Eberli","year":"2009","journal-title":"Methods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1089\/ten.tea.2008.0194","article-title":"A Poly ( Lactic Acid-Co-Caprolactone )\u2013Collagen Hybrid","volume":"15","author":"Ananta","year":"2009","journal-title":"Tissue Eng. Part A"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1097\/01.ju.0000029010.97686.eb","article-title":"Urinary diversion: Ileal conduit to neobladder","volume":"169","author":"Hautmann","year":"2003","journal-title":"J. Urol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/s11934-015-0480-3","article-title":"Tissue-engineered urinary conduits","volume":"16","author":"Kates","year":"2015","journal-title":"Curr. Urol. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1111\/j.1464-410X.2012.11304.x","article-title":"Surgery Illustrated\u2014Surgical Atlas Two-stage hypospadias repair with inner preputial layer Wolfe graft (Aivar Bracka repair)","volume":"110","author":"Altarac","year":"2012","journal-title":"BJU Int."},{"key":"ref_45","first-page":"316","article-title":"Repair of hypospadias using scrotal septum vascular pedicle flap with two wing-like split-thickness skin graft","volume":"23","author":"Duan","year":"2009","journal-title":"Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jpurol.2010.01.016","article-title":"Single-stage dorsal inlay full-thickness genital skin grafts for hypospadias reoperations: Extended follow up","volume":"7","author":"Schwentner","year":"2011","journal-title":"J. Pediatr. Urol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1016\/S0022-5347(01)68105-9","article-title":"A novel inert collagen matrix for hypospadias repair","volume":"162","author":"Atala","year":"1999","journal-title":"J. Urol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.jpurol.2017.03.024","article-title":"Current challenges with proximal hypospadias: We have a long way to go","volume":"13","author":"Gong","year":"2017","journal-title":"J. Pediatr. Urol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1016\/S0022-5347(01)62290-0","article-title":"Reservoir calculi: A comparison of reservoirs constructed from stomach and other enteric segments","volume":"160","author":"Kaefer","year":"1998","journal-title":"J. Urol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/S0022-5347(17)37517-1","article-title":"Metabolic complications of urinary intestinal diversion","volume":"147","author":"McDougal","year":"1992","journal-title":"J. Urol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"109","DOI":"10.5213\/inj.2011.15.3.109","article-title":"Regenerative medicine strategies for treating neurogenic bladder","volume":"15","author":"Yoo","year":"2011","journal-title":"Int. Neurourol. J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3892\/ol.2015.3161","article-title":"Bladder reconstruction: The past, present and future","volume":"10","author":"Khattak","year":"2015","journal-title":"Oncol. Lett."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1177\/1535370213517615","article-title":"Human urinary bladder regeneration through tissue engineering\u2014An analysis of 131 clinical cases","volume":"239","author":"Pokrywczynska","year":"2014","journal-title":"Exp. Biol. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/S0022-5347(17)32676-9","article-title":"Phenotypic and cytogenetic characterization of human bladder urothelia expanded in vitro","volume":"152","author":"Cilento","year":"1994","journal-title":"J. Urol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.eururo.2008.03.068","article-title":"Generation of a functional, differentiated porcine urothelial tissue in vitro","volume":"54","author":"Turner","year":"2008","journal-title":"Eur. Urol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1097\/01.ju.0000108800.47594.8b","article-title":"Characterization of neuropathic bladder smooth muscle cells in culture","volume":"171","author":"Lin","year":"2004","journal-title":"J. Urol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2014","DOI":"10.1016\/j.juro.2011.07.031","article-title":"Tissue engineering potential of urothelial cells from diseased bladders","volume":"186","author":"Subramaniam","year":"2011","journal-title":"J. Urol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1016\/S0022-5347(05)66980-7","article-title":"Bladder epithelial cells from patients with interstitial cystitis produce an inhibitor of heparin-binding epidermal growth factor-like growth factor production","volume":"164","author":"Keay","year":"2000","journal-title":"J. Urol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1016\/S0090-4295(03)00005-0","article-title":"Decreased in vitro proliferation of bladder epithelial cells from patients with interstitial cystitis","volume":"61","author":"Keay","year":"2003","journal-title":"Urology"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1111\/j.1464-410X.2003.04723.x","article-title":"Tissue-engineered buccal mucosa for substitution urethroplasty","volume":"93","author":"Bhargava","year":"2004","journal-title":"BJU Int."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/j.eururo.2008.01.061","article-title":"Tissue-engineered buccal mucosa urethroplasty-clinical outcomes","volume":"53","author":"Bhargava","year":"2008","journal-title":"Eur. Urol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"4003","DOI":"10.1073\/pnas.1220764110","article-title":"Cotransplantation with specific populations of spina bifida bone marrow stem\/progenitor cells enhances urinary bladder regeneration","volume":"110","author":"Sharma","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/scrt458","article-title":"Urine-derived stem cells for potential use in bladder repair","volume":"5","author":"Qin","year":"2014","journal-title":"Stem Cell Res. Ther."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"237","DOI":"10.4103\/jiaps.JIAPS_77_18","article-title":"Tissue Engineering and Stem Cell Therapy in Pediatric Urology","volume":"24","author":"Sharma","year":"2019","journal-title":"J. Indian Assoc. Pediatr. Surg."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1089\/ten.tea.2009.0625","article-title":"Differentiation of human bone marrow mesenchymal stem cells into bladder cells: Potential for urological tissue engineering","volume":"16","author":"Tian","year":"2010","journal-title":"Tissue Eng. Part A"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1016\/j.urology.2011.06.036","article-title":"Construction and evaluation of urinary bladder bioreactor for urologic tissue-engineering purposes","volume":"78","author":"Davis","year":"2011","journal-title":"Urology"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1016\/j.transproceed.2012.01.144","article-title":"Morphological and urodynamic evaluation of urinary bladder wall regeneration: Muscles guarantee contraction but not proper function--a rat model research study","volume":"44","author":"Adamowicz","year":"2012","journal-title":"Transplant Proc."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1111\/j.1464-410X.2008.08129.x","article-title":"Nerve growth factor combined with vascular endothelial growth factor enhances regeneration of bladder acellular matrix graft in spinal cord injury-induced neurogenic rat bladder","volume":"103","author":"Kikuno","year":"2009","journal-title":"BJU Int."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"87","DOI":"10.5173\/ceju.2011.02.art8","article-title":"Schwann cells\u2014A new hope in tissue engineered urinary bladder innervation. A method of cell isolation","volume":"64","author":"Adamowicz","year":"2011","journal-title":"Cent. Eur. J. Urol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"8662","DOI":"10.1038\/s41598-019-45221-z","article-title":"Prenatal diagnosis of hypospadias with 2-dimensional and 3-dimensional ultrasonography","volume":"9","author":"Li","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"484.e1","DOI":"10.1016\/j.jpurol.2017.02.022","article-title":"Ultrasound diagnosis of fetal hypospadias: Accuracy and outcomes","volume":"13","author":"Epelboym","year":"2017","journal-title":"J. Pediatr. Urol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"152.e1","DOI":"10.1016\/j.jpurol.2015.12.002","article-title":"Worldwide prevalence of hypospadias","volume":"12","author":"Springer","year":"2016","journal-title":"J. Pediatr. Urol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.juro.2018.07.038","article-title":"Hypospadias\u2014Nature and Nurture","volume":"200","author":"Kaefer","year":"2018","journal-title":"J. Urol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/j.juro.2012.09.116","article-title":"Association Between Organic Dietary Choice During Pregnancy and Hypospadias in Offspring: A Study of Mothers of 306 Boys Operated on for Hypospadias","volume":"189","author":"Christensen","year":"2013","journal-title":"J. Urol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1016\/j.eururo.2015.05.008","article-title":"Is Hypospadias Associated with Prenatal Exposure to Endocrine Disruptors? A French Collaborative Controlled Study of a Cohort of 300 Consecutive Children Without Genetic Defect","volume":"68","author":"Kalfa","year":"2015","journal-title":"Eur. Urol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"151080","DOI":"10.1016\/j.sempedsurg.2021.151080","article-title":"Hypospadias","volume":"30","author":"Kaefer","year":"2021","journal-title":"Semin. Pediatr. Surg."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.jpedsurg.2005.11.059","article-title":"Hypospadias: Anatomy, etiology, and technique","volume":"41","author":"Baskin","year":"2006","journal-title":"J. Pediatr. Surg."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.eururo.2010.01.012","article-title":"Morbidity of oral mucosa graft harvesting from a single cheek","volume":"58","author":"Barbagli","year":"2010","journal-title":"Eur. Urol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/S0022-5347(05)64060-8","article-title":"Urethral stricture repair with an off-the-shelf collagen matrix","volume":"169","author":"Retik","year":"2003","journal-title":"J. Urol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1016\/j.juro.2007.11.101","article-title":"Randomized Comparative Study Between Buccal Mucosal and Acellular Bladder Matrix Grafts in Complex Anterior Urethral Strictures","volume":"179","author":"AbouShwareb","year":"2008","journal-title":"J. Urol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1016\/j.urology.2014.07.044","article-title":"Reconstruction of penile urethra with the 3-dimensional porous bladder acellular matrix in a rabbit model","volume":"84","author":"Huang","year":"2014","journal-title":"Urology"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1111\/j.1464-410X.2006.06691.x","article-title":"Urethral replacement using epidermal cell-seeded tubular acellular bladder collagen matrix","volume":"99","author":"Fu","year":"2007","journal-title":"BJU Int."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1016\/j.juro.2008.06.013","article-title":"Urethral reconstruction using oral keratinocyte seeded bladder acellular matrix grafts","volume":"180","author":"Li","year":"2008","journal-title":"J. Urol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"015002","DOI":"10.1088\/1748-605X\/aae5e2","article-title":"Prevascularized bladder acellular matrix hydrogel\/silk fibroin composite scaffolds promote the regeneration of urethra in a rabbit model","volume":"14","author":"Cao","year":"2019","journal-title":"Biomed. Mater."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"5038","DOI":"10.1039\/C9TB00530G","article-title":"Small intestinal submucosa: Superiority, limitations and solutions, and its potential to address bottlenecks in tissue repair","volume":"7","author":"Cao","year":"2019","journal-title":"J. Mater. Chem. B"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1097\/01.ju.0000146554.79487.7f","article-title":"Endoscopic urethroplasty with unseeded small intestinal submucosa collagen matrix grafts: A pilot study","volume":"173","year":"2005","journal-title":"J. Urol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.urology.2006.02.044","article-title":"Small intestine submucosa in urethral stricture repair in a consecutive series","volume":"68","author":"Hauser","year":"2006","journal-title":"Urology"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1002\/jbm.b.30170","article-title":"Porcine small intestine submucosa (SIS) is not an acellular collagenous matrix and contains porcine DNA: Possible implications in human implantation","volume":"73","author":"Zheng","year":"2005","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1016\/j.eururo.2006.12.016","article-title":"Small Intestinal Submucosa (SIS) Graft Urethroplasty: Short-term Results","volume":"51","author":"Palminteri","year":"2007","journal-title":"Eur. Urol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1016\/j.eururo.2007.01.099","article-title":"Porcine Small Intestinal Submucosa Graft for Repair of Anterior Urethral Strictures","volume":"51","author":"Fiala","year":"2007","journal-title":"Eur. Urol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.aju.2013.09.003","article-title":"The use of small intestinal submucosa graft for hypospadias repair: Pilot study","volume":"11","author":"Orabi","year":"2013","journal-title":"Arab J. Urol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"691131","DOI":"10.3389\/fped.2021.691131","article-title":"Creation of Tissue-Engineered Urethras for Large Urethral Defect Repair in a Rabbit Experimental Model","volume":"9","author":"Amesty","year":"2021","journal-title":"Front. Pediatr."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.jpurol.2006.01.018","article-title":"Autologous in vitro cultured urothelium in hypospadias repair{star, open}","volume":"3","author":"Fossum","year":"2007","journal-title":"J. Pediatr. Urol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1111\/j.1651-2227.2012.02659.x","article-title":"Prepubertal follow-up after hypospadias repair with autologous in vitro cultured urothelial cells","volume":"101","author":"Fossum","year":"2012","journal-title":"Acta Paediatr. Int. J. Paediatr."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"2041731421998840","DOI":"10.1177\/2041731421998840","article-title":"Augmentation of the insufficient tissue bed for surgical repair of hypospadias using acellular matrix grafts: A proof of concept study","volume":"12","author":"Morgante","year":"2021","journal-title":"J. Tissue Eng."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1007\/s11255-009-9532-2","article-title":"Application of amniotic membrane as xenograft for urethroplasty in rabbit","volume":"41","author":"Shakeri","year":"2009","journal-title":"Int. Urol. Nephrol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.urology.2015.09.014","article-title":"A Novel Approach to Penile Augmentation Urethroplasty Using Buccal Mucosa and Amniotic Membrane: A Pilot Study in a Rabbit Model","volume":"87","author":"Altok","year":"2016","journal-title":"Urology"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1007\/s11255-014-0684-3","article-title":"The application of tissue-engineered preputial matrix and fibrin sealant for urethral reconstruction in rabbit model","volume":"46","author":"Kajbafzadeh","year":"2014","journal-title":"Int. Urol. Nephrol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1007\/s10439-017-1857-x","article-title":"Future Prospects for Human Tissue Engineered Urethra Transplantation: Decellularization and Recellularization-Based Urethra Regeneration","volume":"45","author":"Kajbafzadeh","year":"2017","journal-title":"Ann. Biomed. Eng."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"41934","DOI":"10.1038\/srep41934","article-title":"Acellular Urethra Bioscaffold: Decellularization of Whole Urethras for Tissue Engineering Applications","volume":"7","author":"Vale","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_101","first-page":"1783","article-title":"Urethral replacement using cell seeded tubularized collagen matrices","volume":"168","author":"Yoo","year":"2002","journal-title":"J. Urol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actbio.2017.02.005","article-title":"Tubular collagen scaffolds with radial elasticity for hollow organ regeneration","volume":"52","author":"Versteegden","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1016\/j.juro.2011.12.059","article-title":"Two-layer tissue engineered urethra using oral epithelial and muscle derived cells","volume":"187","author":"Mikami","year":"2012","journal-title":"J. Urol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.biomaterials.2016.01.032","article-title":"Structural and functional evaluation of oxygenating keratin\/silk fibroin scaffold and initial assessment of their potential for urethral tissue engineering","volume":"84","author":"Lv","year":"2016","journal-title":"Biomaterials"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/S0090-4295(02)01642-4","article-title":"Adsorbed To Surface-Modified Polymers","volume":"60","author":"Bisson","year":"2002","journal-title":"Urology"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/0090-4295(84)90107-9","article-title":"Replacement of partially resected canine urethra by polytetrafluoroethylene","volume":"24","author":"Anwar","year":"1984","journal-title":"Urology"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/S0022-5347(17)35728-2","article-title":"One-step treatment of proximal hypospadias by the autologous graft of cultured urethral epithelium","volume":"150","author":"Romagnoli","year":"1993","journal-title":"J. Urol."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"323","DOI":"10.3109\/00365599209181220","article-title":"Urethral reconstruction with a new synthetic absorbable device: An experimental study","volume":"26","author":"Olsen","year":"1992","journal-title":"Scand. J. Urol. Nephrol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"3444","DOI":"10.1098\/rsif.2012.0458","article-title":"Characterizing and optimizing poly-L-lactide-co-1-caprolactone membranes for urothelial tissue engineering","volume":"9","author":"Sartoneva","year":"2012","journal-title":"J. R. Soc. Interface"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1098\/rsif.2010.0520","article-title":"Comparison of a poly-L-lactide-co-\u03b5-caprolactone and human amniotic membrane for urothelium tissue engineering applications","volume":"8","author":"Sartoneva","year":"2011","journal-title":"J. R. Soc. Interface"},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Lv, X., Guo, Q., Han, F., Chen, C., Ling, C., Chen, W., and Li, B. (2016). Electrospun poly(L-lactide)\/poly(ethylene glycol) scaffolds seeded with human amniotic mesenchymal stem cells for urethral epithelium repair. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17081262"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1016\/S0140-6736(10)62354-9","article-title":"Tissue-engineered autologous urethras for patients who need reconstruction: An observational study","volume":"377","author":"Esquiliano","year":"2011","journal-title":"Lancet"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"774","DOI":"10.3389\/fbioe.2020.00774","article-title":"Effective Reconstruction of Functional Urethra Promoted With ICG-001 Delivery Using Core-Shell Collagen\/Poly(Llactide-co-caprolactone) [P(LLA-CL)] Nanoyarn-Based Scaffold: A Study in Dog Model","volume":"8","author":"Zhang","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1039\/C9TB01915D","article-title":"Designing a multifaceted bio-interface nanofiber tissue-engineered tubular scaffold graft to promote neo-vascularization for urethral regeneration","volume":"8","author":"Niu","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.actbio.2016.12.008","article-title":"3D bioprinting of urethra with PCL\/PLCL blend and dual autologous cells in fibrin hydrogel: An in vitro evaluation of biomimetic mechanical property and cell growth environment","volume":"50","author":"Zhang","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1016\/S0022-5347(05)64433-3","article-title":"The use of small intestinal submucosa as an off-the-shelf urethral sling material for pediatric urinary incontinence","volume":"168","author":"Colvert","year":"2002","journal-title":"J. Urol."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1001\/jama.1984.03350050038023","article-title":"Predictive value of urodynamic evaluation in newborns with myelodysplasia","volume":"252","author":"Bauer","year":"1984","journal-title":"JAMA"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.1016\/S0022-5347(01)64766-9","article-title":"Neurogenic bladder dysfunction due to myelomeningocele: Neonatal versus childhood treatment","volume":"157","author":"Wu","year":"1997","journal-title":"J. Urol."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.ucl.2018.06.005","article-title":"Neuropathic Bladder and Augmentation Cystoplasty","volume":"45","author":"Roth","year":"2018","journal-title":"Urol. Clin. N. Am."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/j.urology.2006.04.030","article-title":"Bladder acellular matrix grafting regenerates urinary bladder in the spinal cord injury rat","volume":"68","author":"Obara","year":"2006","journal-title":"Urology"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s00345-006-0142-7","article-title":"Functional improvement in spinal cord injury-induced neurogenic bladder by bladder augmentation using bladder acellular matrix graft in the rat","volume":"25","author":"Urakami","year":"2007","journal-title":"World J. Urol."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1016\/j.juro.2013.10.103","article-title":"Autologous cell seeded biodegradable scaffold for augmentation cystoplasty: Phase II study in children and adolescents with spina bifida","volume":"191","author":"Joseph","year":"2014","journal-title":"J. Urol."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/S0022-5347(17)66624-2","article-title":"Experience with urinary bladder regeneration","volume":"77","author":"Bohne","year":"1957","journal-title":"J. Urol."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1111\/j.1464-410X.1958.tb06231.x","article-title":"Vesical regeneration in the human after total cystectomy and implantation of a plastic mould","volume":"30","author":"Blanco","year":"1958","journal-title":"Br. J. Urol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1111\/j.1464-410X.1964.tb09488.x","article-title":"Formation of A Bladder By A Plastic Shell After Total Cystectomy","volume":"36","author":"Tsulukidze","year":"1964","journal-title":"Br. J. Urol."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/S0022-5347(17)66428-0","article-title":"The use of polyvinyl sponge for experimental cystoplasty","volume":"78","author":"Kudish","year":"1957","journal-title":"J. Urol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0022-5347(17)62828-3","article-title":"Clinical experiences of bladder reconstruction using preserved bladder and gelatin sponge bladder in the case of bladder cancer","volume":"98","author":"Tsuji","year":"1967","journal-title":"J. Urol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0022-5347(17)61680-X","article-title":"Enlargement of contracted bladder by use of gelatin sponge bladder","volume":"104","author":"Orikasa","year":"1970","journal-title":"J. Urol."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/S0022-5347(17)58181-1","article-title":"Cytoplasty by regeneration of the bladder","volume":"118","author":"Taguchi","year":"1977","journal-title":"J. Urol."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/S0022-5347(17)61813-5","article-title":"Experimental investigations of bladder regeneration using teflon-felt as a bladder wall substitute","volume":"104","author":"Dustmann","year":"1970","journal-title":"J. Urol."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s00383-012-3063-0","article-title":"Bladder augmentation using acellular collagen biomatrix: A pilot experience in exstrophic patients","volume":"28","author":"Caione","year":"2012","journal-title":"Pediatr. Surg. Int."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"150","DOI":"10.4111\/kju.2011.52.2.150","article-title":"Bladder reconstruction using bovine pericardium in a case of enterovesical fistula","volume":"52","author":"Moon","year":"2011","journal-title":"Korean J. Urol."},{"key":"ref_133","first-page":"172","article-title":"Augmentation duracystoplasty in neurogenic bladder dysfunction","volume":"2","author":"Arikan","year":"1995","journal-title":"Int. J. Urol. Off. J. Jpn. Urol. Assoc."},{"key":"ref_134","first-page":"195","article-title":"Alloplastic replacement of the urinary bladder wall with lyophilized human dura","volume":"2","author":"Korb","year":"1970","journal-title":"Eur. Surg. Res. Eur. Chir. Forschung. Rech. Chir. Eur."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1159\/000455617","article-title":"Duraplasty of the urinary bladder--results after two to six years","volume":"1","year":"1975","journal-title":"Eur. Urol."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/S0022-5347(01)65761-6","article-title":"Characterization of small intestinal submucosa regenerated canine detrusor: Assessment of reinnervation, in vitro compliance and contractility","volume":"156","author":"Kropp","year":"1996","journal-title":"J. Urol."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1007\/s003450050064","article-title":"Small-intestinal submucosa for bladder augmentation: A review of preclinical studies","volume":"16","author":"Kropp","year":"1998","journal-title":"World J. Urol."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/S0090-4295(99)80227-1","article-title":"Experimental assessment of small intestinal submucosa as a bladder wall substitute","volume":"46","author":"Kropp","year":"1995","journal-title":"Urology"},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s00383-009-2521-9","article-title":"Urinary bladder auto augmentation using INTEGRA and SURGISIS: An experimental model","volume":"26","author":"Barker","year":"2010","journal-title":"Pediatr. Surg. Int."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.juro.2014.01.116","article-title":"Tissue engineered cystoplasty augmentation for treatment of neurogenic bladder using small intestinal submucosa: An exploratory study","volume":"192","author":"Zhang","year":"2014","journal-title":"J. Urol."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1016\/S0022-5347(05)67207-2","article-title":"Laparoscopic augmentation cystoplasty with different biodegradable grafts in an animal model","volume":"164","author":"Portis","year":"2000","journal-title":"J. Urol."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"2450","DOI":"10.1097\/01.ju.0000127756.64619.27","article-title":"Laparoscopic mid sagittal hemicystectomy and bladder reconstruction with small intestinal submucosa and reimplantation of ureter into small intestinal submucosa: 1-Year followup","volume":"171","author":"Landman","year":"2004","journal-title":"J. Urol."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1097\/01.ju.0000161592.00434.c1","article-title":"Bladder reconstitution with bone marrow derived stem cells seeded on small intestinal submucosa improves morphological and molecular composition","volume":"174","author":"Chung","year":"2005","journal-title":"J. Urol."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1002\/stem.568","article-title":"A Nonhuman primate model for urinary bladder regeneration using autologous sources of bone marrow-derived mesenchymal stem cells","volume":"29","author":"Sharma","year":"2011","journal-title":"Stem Cells"},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/j.urology.2004.11.024","article-title":"Human embryoid body-derived stem cells in bladder regeneration using rodent model","volume":"65","author":"Frimberger","year":"2005","journal-title":"Urology"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0090-4295(97)00644-4","article-title":"Bladder augmentation using allogenic bladder submucosa seeded with cells","volume":"51","author":"Yoo","year":"1998","journal-title":"Urology"},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1089\/ten.tea.2011.0609","article-title":"Coadministration of platelet-derived growth factor-BB and vascular endothelial growth factor with bladder acellular matrix enhances smooth muscle regeneration and vascularization for bladder augmentation in a rabbit model","volume":"19","author":"Zhou","year":"2013","journal-title":"Tissue Eng. Part A"},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1089\/scd.2015.0246","article-title":"Bladder Acellular Matrix Grafts Seeded with Adipose-Derived Stem Cells and Incubated Intraperitoneally Promote the Regeneration of Bladder Smooth Muscle and Nerve in a Rat Model of Bladder Augmentation","volume":"25","author":"Zhe","year":"2016","journal-title":"Stem Cells Dev."},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"2226","DOI":"10.1016\/j.jpedsurg.2019.11.020","article-title":"In-vivo regeneration of bladder muscular wall with whole decellularized bladder matrix: A novel hourglass technique for duplication of bladder volume in rabbit model","volume":"55","author":"Sabetkish","year":"2020","journal-title":"J. Pediatr. Surg."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.actbio.2015.05.032","article-title":"Time-dependent bladder tissue regeneration using bilayer bladder acellular matrix graft-silk fibroin scaffolds in a rat bladder augmentation model","volume":"23","author":"Zhao","year":"2015","journal-title":"Acta Biomater."},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/j.biomaterials.2010.09.051","article-title":"Evaluation of gel spun silk-based biomaterials in a murine model of Bladder Augmentation","volume":"32","author":"Mauney","year":"2013","journal-title":"Biomaterials"},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"7562","DOI":"10.1016\/j.biomaterials.2011.06.067","article-title":"The effect of manipulation of silk scaffold fabrication parameters on matrix performance in a murine model of bladder augmentation","volume":"32","author":"Gomez","year":"2011","journal-title":"Biomaterials"},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"4758","DOI":"10.1016\/j.biomaterials.2013.03.038","article-title":"The performance of silk scaffolds in a rat model of augmentation cystoplasty","volume":"34","author":"Seth","year":"2013","journal-title":"Biomaterials"},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"8681","DOI":"10.1016\/j.biomaterials.2013.08.001","article-title":"Bladder tissue regeneration using acellular bi-layer silk scaffolds in a large animal model of augmentation cystoplasty","volume":"34","author":"Tu","year":"2013","journal-title":"Biomaterials"},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"7452","DOI":"10.1016\/j.biomaterials.2014.05.044","article-title":"The use of bi-layer silk fibroin scaffolds and small intestinal submucosa matrices to support bladder tissue regeneration in a rat model of spinal cord injury","volume":"35","author":"Chung","year":"2014","journal-title":"Biomaterials"},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1016\/j.juro.2006.04.085","article-title":"Bladder Augmentation With Acellular Dermal Biomatrix in a Diseased Animal Model","volume":"176","author":"Akbal","year":"2006","journal-title":"J. Urol."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/bju.12591","article-title":"Tissue engineering of diseased bladder using a collagen scaffold in a bladder exstrophy model","volume":"114","author":"Roelofs","year":"2014","journal-title":"BJU Int."},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.actbio.2016.06.010","article-title":"IGF-1-containing multi-layered collagen-fibrin hybrid scaffolds for bladder tissue engineering","volume":"41","author":"Vardar","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s12967-016-1112-5","article-title":"Two differentially structured collagen scaffolds for potential urinary bladder augmentation: Proof of concept study in a G\u00f6ttingen minipig model","volume":"15","author":"Stollenwerk","year":"2017","journal-title":"J. Transl. Med."},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1007\/s13770-019-00209-8","article-title":"Bladder Augmentation Using Lyoplant\u00ae: First Experimental Results in Rats","volume":"16","author":"Winde","year":"2019","journal-title":"Tissue Eng. Regen. 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