{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T21:06:58Z","timestamp":1761253618544,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T00:00:00Z","timestamp":1654732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"JSPS KAKENHI","doi-asserted-by":"publisher","award":["JP19K22602","JP21H03080","JPMJSP2123"],"award-info":[{"award-number":["JP19K22602","JP21H03080","JPMJSP2123"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"JST SPRING","doi-asserted-by":"publisher","award":["JP19K22602","JP21H03080","JPMJSP2123"],"award-info":[{"award-number":["JP19K22602","JP21H03080","JPMJSP2123"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mesenchymal stem cells (MSCs) affect immune cells and exert anti-inflammatory effects. Human amniotic fluid stem cells (hAFSCs), a type of MSCs, have a high therapeutic effect in animal models of inflammation-related diseases. hAFSCs can be easily isolated and cultured from amniotic fluid, which is considered a medical waste. Hence, amniotic fluid can be a source of cells for MSC therapy of inflammatory diseases. However, the effect of hAFSCs on acquired immunity in vivo, especially on regulatory T cells, has not yet been fully elucidated. Therefore, in this study, we aimed to understand the effects of hAFSCs on acquired immunity, particularly on regulatory T cells. We showed that hAFSCs ameliorated the thioglycollate-induced inflammation by forming aggregates with host immune cells, such as macrophages, T cells, and B cells in the peritoneal cavity. Further, the regulatory T cells increased in the peritoneal cavity. These results indicated that, in addition to helping the innate immunity, hAFSCs could also aid the acquired immune system in vivo against inflammation-related diseases by increasing regulatory T cells.<\/jats:p>","DOI":"10.3390\/ijms23126433","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T02:25:33Z","timestamp":1654827933000},"page":"6433","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Human Amniotic Fluid Stem Cells Ameliorate Thioglycollate-Induced Peritonitis by Increasing Tregs in Mice"],"prefix":"10.3390","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0845-320X","authenticated-orcid":false,"given":"Yushi","family":"Abe","sequence":"first","affiliation":[{"name":"Department of Obstetrics & Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daigo","family":"Ochiai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masako","family":"Taguchi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9292-6542","authenticated-orcid":false,"given":"Seiji","family":"Kanzaki","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan"},{"name":"StemCell Institute Inc., Tokyo 105-0004, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3652-5389","authenticated-orcid":false,"given":"Satoru","family":"Ikenoue","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshifumi","family":"Kasuga","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamoru","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Obstetrics & Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/8756-3282(92)90364-3","article-title":"Characterization of cells with osteogenic potential from human marrow","volume":"13","author":"Haynesworth","year":"1992","journal-title":"Bone"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1089\/107632701300062859","article-title":"Multilineage cells from human adipose tissue: Implications for cell-based therapies","volume":"7","author":"Zuk","year":"2001","journal-title":"Tissue Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1634\/stemcells.21-1-105","article-title":"Searching for alternative sources of postnatal human mesenchymal stem cells: Candidate MSC-like cells from umbilical cord","volume":"21","author":"Romanov","year":"2003","journal-title":"Stem Cells"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1634\/stemcells.2004-0058","article-title":"Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta","volume":"22","author":"Scherjon","year":"2004","journal-title":"Stem Cells"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/nbt1274","article-title":"Isolation of amniotic stem cell lines with potential for therapy","volume":"25","author":"Bartsch","year":"2007","journal-title":"Nat. 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