{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T16:10:46Z","timestamp":1783354246326,"version":"3.54.6"},"reference-count":59,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T00:00:00Z","timestamp":1654819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union","award":["T1E\u2206K-03798"],"award-info":[{"award-number":["T1E\u2206K-03798"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Syndecans act as independent co-receptors to exert biological activities and their altered function is associated with many pathophysiological conditions. Here, syndecan-1 and -4 were examined in lesional skin of patients with psoriasis. Immunohistochemical staining confirmed altered syndecan-1 distribution and revealed absence of syndecan-4 expression in the epidermis. Fibronectin (FN)\u2014known to influence inflammation and keratinocyte hyperproliferation via \u03b15\u03b21 integrin in psoriasis\u2014was also decreased. Syndecan-1 and -4 expression was analyzed in freshly isolated lesional psoriatic human keratinocytes (PHK) characterized based on their proliferation and differentiation properties. mRNA levels of syndecan-1 were similar between healthy and PHK, while syndecan-4 was significantly decreased. Cell growth and release of the pro-inflammatory Tumor Necrosis Factor-alpha (TNF\u03b1) were selectively and significantly induced in PHKs plated on FN. Results from co-culture of healthy keratinocytes and psoriatic fibroblasts led to the speculation that at least one factor released by fibroblasts down-regulate syndecan-1 expression in PHK plated on FN. To assay if biological treatments for psoriasis target keratinocyte proliferation, gelatin-based patches enriched with inteleukin (IL)-17\u03b1 or TNF\u03b1 blockers were prepared and tested using a full-thickness healthy epidermal model (Phenion\u00ae). Immunohistochemistry analysis showed that both blockers impacted the localisation of syndecan-1 within the refined epidermis. These results provide evidence that syndecans expression are modified in psoriasis, suggesting that they may represent markers of interest in this pathology.<\/jats:p>","DOI":"10.3390\/ijms23126511","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T10:25:12Z","timestamp":1654856712000},"page":"6511","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Altered Distribution and Expression of Syndecan-1 and -4 as an Additional Hallmark in Psoriasis"],"prefix":"10.3390","volume":"23","author":[{"given":"Eleni","family":"Koliakou","sequence":"first","affiliation":[{"name":"Laboratory of Histology and Embryology, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7075-878X","authenticated-orcid":false,"given":"Manthou","family":"Eleni","sequence":"additional","affiliation":[{"name":"Laboratory of Histology and Embryology, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioanna","family":"Koumentakou","sequence":"additional","affiliation":[{"name":"Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0751-2802","authenticated-orcid":false,"given":"Nikolaos","family":"Bikiaris","sequence":"additional","affiliation":[{"name":"Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0597-6216","authenticated-orcid":false,"given":"Polyanthi","family":"Konstantinidou","sequence":"additional","affiliation":[{"name":"Department of Pathology, Forensic Service of Thessaloniki, 57003 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Rousselle","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie Tissulaire et Ing\u00e9nierie Th\u00e9rapeutique, UMR 5305, CNRS, University Lyon 1, SFR BioSciences, 7 Passage du Vercors, 69367 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8004-7666","authenticated-orcid":false,"given":"Doxakis","family":"Anestakis","sequence":"additional","affiliation":[{"name":"Department of Pathology, Forensic Service of Thessaloniki, 57003 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4072-3591","authenticated-orcid":false,"given":"Elisabeth","family":"Lazaridou","sequence":"additional","affiliation":[{"name":"2nd Department of Dermatology-Venereology, Medical School, Papageorgiou Hospital, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelia","family":"Kalloniati","sequence":"additional","affiliation":[{"name":"2nd Department of Dermatology-Venereology, Medical School, Papageorgiou Hospital, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimosthenis","family":"Miliaras","sequence":"additional","affiliation":[{"name":"Laboratory of Histology and Embryology, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4367-9370","authenticated-orcid":false,"given":"Anna","family":"Michopoulou","sequence":"additional","affiliation":[{"name":"Biohellenika Biotechnology Company, Leoforos Georgikis Scholis 65, 55535 Thessaloniki, Greece"},{"name":"Laboratory of Biological Chemistry, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1016\/j.autrev.2017.07.011","article-title":"Autoimmune aspects of psoriasis: Heritability and autoantigens","volume":"16","author":"Prinz","year":"2017","journal-title":"Autoimmun. 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